1,779 results

1. pHJ4

   Plasmid

   #158785

   Purpose

   Pan-neuronal nuclear-localized GCaMP expression in C. elegans

   Depositor

   Zachary Knight, Jennifer Garrison

   Article

   Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

   Insert

   Prab-3::nls::GCaMP6m
   

   Tags

   NLS

   Expression

   Worm

   Available Since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJW1884

   Plasmid

   #154332

   Purpose

   sgRNA Target Vector for CRISPR

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   cxTi10882 site targeting sgRNA (F+E) with R07E5.16 U6 promoter and 3'UTR
   

   Use

   CRISPR

   Expression

   Worm

   Mutation

   F+E mutations in sgRNA

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJW1827

   Plasmid

   #154323

   Purpose

   Multi-cassette for SapTrap

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa linker::mScarlet-I
   

   Use

   CRISPR

   Expression

   Worm

   Available Since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJW1841

   Plasmid

   #154338

   Purpose

   Promoter reporter

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   SV40 NLS::mScarlet_I(dpi)::-tbb-2 3'UTR
   

   Expression

   Worm

   Mutation

   Silent mutations to remove piRNA sites

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJW1948

   Plasmid

   #154340

   Purpose

   Enhancer reporter

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   pes-10delta::SV40 NLS::mScarlet_I::PEST(dpi)::-tbb-2 3'UTR
   

   Expression

   Worm

   Mutation

   Silent mutations to remove piRNA sites

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDD289

   Plasmid

   #137018

   Purpose

   GFP-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Bob Goldstein, Daniel Dickinson

   Insert

   GFP-GLO^SEC^3xFlag + ccdB
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFlag and C. elegans GLO-optimized GFP

   Expression

   Worm

   Available Since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDD376

   Plasmid

   #137022

   Purpose

   SapTrap mKate2-GLO donor

   Depositor

   Bob Goldstein, Daniel Dickinson

   Insert

   C. elegans GLO-optimized mKate2
   

   Use

   CRISPR; Saptrap donor for cloning repair templat…

   Tags

   C. elegans GLO-optimized mKate2

   Expression

   Worm

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXF90

   Plasmid

   #139044

   Purpose

   MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

   Depositor

   Erik Griffin

   Article

   Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

   Insert

   halotag::HDEL (includes STOP codon, PATC introns)
   

   Expression

   Worm

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCZGY921

   Plasmid

   #135092

   Purpose

   Transcriptional GFP reporter of acr-2 (cholinergic) expressing neurons in C. elegans

   Depositor

   Yishi Jin

   Article

   Jospin et al PLoS Biol. 2009 Dec;7(12):e1000265. doi:

   Insert

   Pacr-2
   

   Tags

   GFP and Synthetic Intron

   Expression

   Worm

   Promoter

   Pacr-2

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCZGY2750

    Plasmid

    #135094

    Purpose

    Site specific CRISPR/Cas9 editing of C. elegans Chr IV

    Depositor

    Yishi Jin

    Article

    Andrusiak et al Neuron. 2019 Jul 24. pii: S0896-6273(19)

    Insert

    sgRNA for cxTi10082 (actgttggatgcctgtgtag)
    

    Use

    CRISPR

    Expression

    Worm

    Promoter

    U6

    Available Since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJPL3[flp-6p::cmk-1::GFP]

    Plasmid

    #124613

    Purpose

    Used to confirm the expression of the trans-spliced GFP reporter in the expected ASE neurons

    Depositor

    Anne Brunet

    Article

    Lim et al J Neurosci. 2018 Jul 4;38(27):6114-6129.

    Insert

    [flp-6p::cmk-1::GFP]
    

    Expression

    Worm

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGC684

    Plasmid

    #107022

    Purpose

    GFP reporter of lag-2 driven by 1 kb of promoter, which lackes both DBS and Cons motifs

    Depositor

    Jane Hubbard

    Article

    Pekar et al Development. 2017 Aug 15;144(16):2896-29

    Insert

    GFP-PH
    

    Tags

    GFP

    Expression

    Worm

    Promoter

    lag-2

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGC682

    Plasmid

    #107020

    Purpose

    GFP reporter of lag-2 driven by 1 kb of promoter, which lackes DBS motif

    Depositor

    Jane Hubbard

    Article

    Pekar et al Development. 2017 Aug 15;144(16):2896-29

    Insert

    GFP-PH
    

    Tags

    GFP

    Expression

    Worm

    Promoter

    lag-2

    Available Since

    April 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAS2-his-24

    Plasmid

    #124318

    Purpose

    Vector Backbone for his-24 fusion and let-858 3'UTR

    Depositor

    Andrew Leifer

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAS1-his-24

    Plasmid

    #124317

    Purpose

    Vector Backbone for his-24 fusion and unc-54 3'UTR

    Depositor

    Andrew Leifer

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAS1-rab-3P::his-24::mOrange

    Plasmid

    #124346

    Purpose

    Nuclear Localized Pan-Neuronal mOrange

    Depositor

    Andrew Leifer

    Insert

    rab-3, mOrange (rab-3 Nematode)
    

    Expression

    Worm

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAS1-his-24::GCaMP6s

    Plasmid

    #124333

    Purpose

    Expressing Nuclear Localized GCaMP6s

    Depositor

    Andrew Leifer

    Insert

    GCaMP6s (his-24 Nematode)
    

    Expression

    Worm

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAS1-his-24::mNeptunr

    Plasmid

    #124331

    Purpose

    Expressing Nuclear Localized mNeptune

    Depositor

    Andrew Leifer

    Insert

    mNeptune (his-24 Nematode)
    

    Expression

    Worm

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAS2-his-24::mCherry

    Plasmid

    #124328

    Purpose

    Expressing Nuclear Localized mCherry

    Depositor

    Andrew Leifer

    Insert

    mCherry (his-24 Nematode)
    

    Expression

    Worm

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAS1-his-24::mCherry

    Plasmid

    #124327

    Purpose

    Expressing Nuclear Localized mCherry

    Depositor

    Andrew Leifer

    Insert

    mCherry (his-24 Nematode)
    

    Expression

    Worm

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge