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pMC-XI2

Plasmid

#176156

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-2

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pAG423GAL-ccdB-HA

Plasmid

#14245

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

3HA

Expression

Yeast

Available Since

March 29, 2007

Availability

Academic Institutions and Nonprofits only

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pI1-MTU-DO-HIS

Plasmid

#160142

Purpose

Integrative vector for K. marxianus, targeting the LAC4 locus. Histidine selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pJZC519

Plasmid

#62278

Purpose

Yeast dCas9-VP64 expression plasmid

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

dCas9-VP64

Tags

Has a VP64 fusion

Expression

Yeast

Mutation

Has mutations in the RuvC1 and HNH domains to ren…

Promoter

pTdh3

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAAH5

Plasmid

#41829

Purpose

Used as a PCR template for tagging yeast proteins with the SNAP tag by homologous recombination and selection for hygromycin resistance.

Depositor

Melissa Moore

Article

Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

Type

Empty backbone

Use

Pcr template

Expression

Bacterial and Yeast

Promoter

T7,TEF

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pAG423GPD-ccdB-HA

Plasmid

#14246

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

3HA

Expression

Yeast

Available Since

March 29, 2007

Availability

Academic Institutions and Nonprofits only

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pDS1885

Plasmid

#177341

Purpose

Expresses Aequorin in Candida albicans using the dominant marker SAT1

Depositor

Dominique Sanglard

Article

Sanglard et al J Fungi (Basel). 2021 Apr 20;7(4). pii:

Insert

Aequorin

Expression

Yeast

Promoter

ACT1

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pPTK020-7-attB

Plasmid

#84989

Purpose

Type 7, BxbI recognition site

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

BxbI recognition site

Use

Synthetic Biology

Expression

Yeast

Mutation

BsaI site removed (1711 t to a)

Promoter

Non

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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YIp ADE2-HphMx

Plasmid

#122916

Purpose

ADE2-HphMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Article

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available Since

Oct. 18, 2019

Availability

Academic Institutions and Nonprofits only

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Gma-pCAMBIA-1301-STTM171

Plasmid

#84080

Purpose

Disturb the expression of miR171 in soybean

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR171

Expression

Bacterial, Plant, and Yeast…

Promoter

2x35S

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pPTK006-3a-αMF no EAEA

Plasmid

#84965

Purpose

Type 3a, α-mating factor no EAEA - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

α-mating factor no EAEA secretion tag

Use

Synthetic Biology

Expression

Yeast

Promoter

Non

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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p413GPD-hTPI

Plasmid

#50719

Purpose

expression of human TPI1 in yeast

Depositor

Markus Ralser

Article

Gruning et al Open Biol. 2014 Mar 5;4(3):130232. doi:

Insert

Triosephosphate isomerase 1 (TPI1 Human)

Expression

Yeast

Promoter

GPD

Available Since

Jan. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pKar2-msGFP-HDEL

Plasmid

#117668

Purpose

Localisation of Endoplasmic Reticulum

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pKar2-msGFP-HDEL

Expression

Yeast

Mutation

Contains the Kar2 promoter and signal sequence fr…

Promoter

pKar2

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAV123

Plasmid

#63189

Purpose

pRS423 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Article

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pFTK004

Plasmid

#171276

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

PgpdA ANIA_08041 promoter

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Dec. 2, 2021

Availability

Academic Institutions and Nonprofits only

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LPH2782 - Ub-K48R

Plasmid

#32161

Depositor

Linda Hicke

Insert

synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)

Expression

Yeast

Mutation

K48R

Promoter

CUP1

Available Since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only
pRS316CUP RNQ CFP

Plasmid

#1171

Depositor

Susan Lindquist

Article

Sondheimer et al Mol Cell 2000 Jan;5(1):163-72.

Insert

RNQ1 (RNQ1 Budding Yeast)

Tags

CFP

Expression

Yeast

Available Since

Jan. 21, 2005

Availability

Academic Institutions and Nonprofits only
pCfB6627

Plasmid

#106159

Purpose

EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6682 into Yarrowia lipolytica chromosomal location IntC_2, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

unknown

Expression

Yeast

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pPS2044

Plasmid

#1375

Depositor

Pamela Silver

Article

Brodsky et al RNA 2000 Dec;6(12):1737-49.

Insert

RPL25-U1A-PGK1 3' UTR (RPL25 Budding Yeast)

Expression

Yeast

Available Since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
pHES822

Plasmid

#89193

Purpose

Expresses YFP under control of synthetic promoter containing Zif268 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pZ-YFP

Expression

Yeast

Promoter

pZ

Available Since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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