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Plasmid#181714PurposeGalactose inducible expression of hnRNPA2B1DepositorInserthnRNPA2B1 (HNRNPA2B1 Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG416-GAL-SUP35
Plasmid#181705PurposeGalactose inducible expression of SUP35DepositorInsertSUP35 (SUP35 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-CFAPorig
Plasmid#221092PurposeFAP insert for C-terminally tagging genes of interest (original FAP + 2xMYC tag)DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3
Plasmid#221112PurposeFAP tagged STE3 (N-terminally tagged, optimized for yeast expression) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ERDepositorInsertMFA-IgKappa-FAPoptim-STE3
UseTagsExpressionYeastMutationPromoterSTE3Available sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-STE3-GFP
Plasmid#221113PurposeGFP-tagged STE3 (C-terminally tagged, optimized for yeast expression)DepositorInsertSTE3-GFP
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3-TAILLESS
Plasmid#221117PurposeFAP tagged STE3-TAILLESS mutant (delta288-470 - N-terminally tagged, optimized) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ERDepositorInsertMFA-IgKappa-FAPoptim-STE3-Tailless
UseTagsExpressionYeastMutationSTE3 mutant that lacks the entire C-terminal tail…PromoterSTE3Available sinceMarch 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 E3GFP
Plasmid#233008PurposeGalactose iduced expression of Gcn4 E+GFP in yeastDepositorInsertGcn4 E+
UseTagsHA-GFP-HA and TEV cleavage siteExpressionYeastMutationD105E, D118E, D139EPromoterGal1Available sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
Fyv6-NFLAG del1-51
Plasmid#234136PurposeFirst 51 amino acids deleted of Fyv6 with FLAG epitope tag at N-terminusDepositorInsertFyv6
UseTagsExpressionYeastMutationN-terminus truncationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS424 Prp8 S1584F
Plasmid#234122PurposeSuppressor screen mutant in full-length Prp8 on a TRP plasmidDepositorInsertPrp8
UseTagsExpressionYeastMutationS1584FPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS414-Syf1 817 Truncation
Plasmid#234126PurposeSyf1 PCR truncation starting at 817 through the end of the geneDepositorInsertSyf1
UseTagsExpressionYeastMutationN-terminus truncationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS424 Prp8 S1584Y
Plasmid#234121PurposeSuppressor screen mutant in full-length Prp8 on a TRP plasmidDepositorInsertPrp8
UseTagsExpressionYeastMutationS1584YPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS424 Prp8 V1862L
Plasmid#234123PurposeSuppressor screen mutant in full-length Prp8 on a TRP plasmidDepositorInsertPrp8
UseTagsExpressionYeastMutationV1862LPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 F124IHA
Plasmid#232993PurposeGalactose iduced expression of Gcn4 F124IHA in yeastDepositorInsertGcn4 F124I
UseTags3xHA and TEV cleavage siteExpressionYeastMutationF124IPromoterGal1Available sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_FL Gcn4
Plasmid#232955PurposeGalactose iduced expression of full-length Gcn4 in yeastDepositorInsertGcn4
UseTagsTEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
Fyv6-NFLAG del1-16
Plasmid#234138PurposeFirst 16 amino acids deleted of Fyv6 with FLAG epitope tag at N-terminusDepositorInsertFyv6
UseTagsExpressionYeastMutationN-terminus truncationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
Fyv6-NFLAG del103-173
Plasmid#234134PurposeAmino acids 103-173 deleted in Fyv6 with FLAG epitope tag at N-terminusDepositorInsertFyv6
UseTagsExpressionYeastMutationC-terminus truncationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS414-Fyv6-CFLAG
Plasmid#234133PurposeRestriction digest from pUC18-CFLAG-Fyv6 and ligation into pRS414DepositorInsertFyv6
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
Fyv6-NFLAG del134-173
Plasmid#234135PurposeAmino acids 134-173 deleted in Fyv6 with FLAG epitope tag at N-terminusDepositorInsertFyv6
UseTagsExpressionYeastMutationC-terminus truncationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS414-Fyv6
Plasmid#234140PurposeAmplified Fyv6 from gDNA, cut with SalI and NotI, ligated into pRS414DepositorInsertFyv6
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS414-NFLAG-Fyv6
Plasmid#234139PurposeRestriction digest from pUC18-NFLAG-Fyv6 and ligation into pRS414DepositorInsertFyv6
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS424 Prp8 G1868R
Plasmid#234124PurposeSuppressor screen mutant in full-length Prp8 on a TRP plasmidDepositorInsertPrp8
UseTagsExpressionYeastMutationG1868RPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS416-Fyv6
Plasmid#234141PurposeAmplified Fyv6 from gDNA, cut with SalI and NotI, ligated into pRS416DepositorInsertFyv6
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
Fyv6-NFLAG del1-23
Plasmid#234137PurposeFirst 23 amino acids deleted of Fyv6 with FLAG epitope tag at N-terminusDepositorInsertFyv6
UseTagsExpressionYeastMutationN-terminus truncationPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS314-Cef1-M175I
Plasmid#234118PurposeSuppressor screen mutant in Cef2DepositorInsertCef1
UseTagsExpressionYeastMutationM175IPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS314-Cef1-Q193P
Plasmid#234117PurposeSuppressor screen mutant in Cef1DepositorInsertCef1
UseTagsExpressionYeastMutationQ193PPromoterAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS314-Cef1-A37V
Plasmid#234119PurposeSuppressor screen mutant in Cef3DepositorInsertCef1
UseTagsExpressionYeastMutationA37VPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS416-Syf1
Plasmid#234113PurposeSyf1 region PCR, restriction digest and ligation into pRS416 backboneDepositorInsertSyf1
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS414-Syf1 634 Truncation
Plasmid#234116PurposeSyf1 PCR truncation starting at 634 through the end of the geneDepositorInsertSyf1
UseTagsExpressionYeastMutationN-terminus truncationPromoterAvailable sinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGADT7_Sr62NLRa
Plasmid#233528PurposeExpresses Sr62NLRa in yeastDepositorInsertSr62NLRa
UseTagsGAL4 AD, HA tagExpressionYeastMutationPromoterADH1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS414-Syf1
Plasmid#234114PurposeSyf1 region PCR, restriction digest and ligation into pRS414 backboneDepositorInsertSyf1
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS414-Syf1 778 Truncation
Plasmid#234115PurposeSyf1 PCR truncation starting at 778 through the end of the geneDepositorInsertSyf1
UseTagsExpressionYeastMutationN-terminus truncationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 WTSLFD
Plasmid#232980PurposeGalactose iduced expression of Gcn4 WTSLFD+ in yeastDepositorInsertGcn4 WTSLFD+
UseTags3xHA and TEV cleavage siteExpressionYeastMutationF108W, E109T, Y110S, E111L, N112F, L113DPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Aro2+
Plasmid#232982PurposeGalactose iduced expression of Gcn4 Aro2+ in yeastDepositorInsertGcn4 Aro2+
UseTagsTEV cleavage siteExpressionYeastMutationN116F, S117E, E119N, P129Q, T132YPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Aro3+
Plasmid#232983PurposeGalactose iduced expression of Gcn4 Aro3+ in yeastDepositorInsertGcn4 Aro3+
UseTagsTEV cleavage siteExpressionYeastMutationD103Y, N112T, L113Y, S117F, K140RPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 W120AHA
Plasmid#232992PurposeGalactose iduced expression of Gcn4 W120AHA in yeastDepositorInsertGcn4 W120A
UseTags3xHA and TEV cleavage siteExpressionYeastMutationW120APromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 SATtoG KtoRHA
Plasmid#232994PurposeGalactose iduced expression of Gcn4 SATtoG KtoRHAin yeastDepositorInsertGcn4 SATtoG KtoR
UseTags3xHA and TEV cleavage siteExpressionYeastMutationS101G, T102G, S104G, T105G, S117G, K118R, T121G, …PromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Aro3+HA
Plasmid#232990PurposeGalactose iduced expression of Gcn4 Aro3+HA in yeastDepositorInsertGcn4 Aro3+
UseTags3xHA and TEV cleavage siteExpressionYeastMutationD103Y, N112T, L113Y, S117F, K140RPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_DBDHA
Plasmid#232987PurposeGalactose iduced expression of Gcn4 DBDAHA in yeastDepositorInsertDBD
UseTags3xHA and TEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 WT 44merHA
Plasmid#232988PurposeGalactose iduced expression of Gcn4 WT 44merHA in yeastDepositorInsertGcn4 WT 44mer
UseTags3xHA and TEV cleavage siteExpressionYeastMutationPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAG415Gal_Gcn4 Aro2+HA
Plasmid#232989PurposeGalactose iduced expression of Gcn4 Aro2+HA in yeastDepositorInsertGcn4 Aro2+
UseTags3xHA and TEV cleavage siteExpressionYeastMutationN116F, S117E, E119N, P129Q, T132YPromoterGal1Available sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only