8,058 results

1. NM R(delta) 2-5 GFP

   Plasmid

   #1209

   Depositor

   Susan Lindquist

   Article

   Liu et al Nature 1999 Aug 5;400(6744):573-6.

   Insert

   sup35 NM domain (SUP35 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   last four repeats of sup35 N domain deleted

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMLB10

   Plasmid

   #177273

   Purpose

   GAL1p:T7RNAP(F11L;T613A):CYC1t

   Depositor

   Michael Jensen

   Article

   Jensen et al Nucleic Acids Res. 2021 Sep 7;49(15):e88

   Insert

   T7RNAP(F11L;T613A)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   F11L T613A

   Promoter

   GAL1

   Available since

   April 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAG306GPD-ccdB-EGFP

   Plasmid

   #14188

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCYC1pr-GNSI (LEU)

   Plasmid

   #111450

   Purpose

   GNSI is a synthetic, genetically-encoded reporter that allows rapid plate-based assessment of AP-3 functional deficiency, using either colorimetric or growth phenotype readouts.

   Depositor

   Alexey Merz

   Article

   Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1

   Inserts

   CYC1 Promoter (CYC1 Budding Yeast)
   Nyv1 Cytosolic Domain (aa 6-230)
   Snc1 Transmembrane Domain (TMD)
   SUC2 CDS
   SUC2 terminator
   

   Use

   Tags

   mGFP5

   Expression

   Yeast

   Mutation

   M109I (Naturally occurring SNP) and The first 21 …

   Promoter

   Available since

   June 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRS406-PHO5-GFP-2xOsh2 PH domain [short]

   Plasmid

   #58829

   Purpose

   Expresses GFP-Tagged Osh2 PH domain in yeast

   Depositor

   Tim Levine

   Article

   Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

   Insert

   2xOsh2 PH domain [short] (OSH2 Budding Yeast)
   

   Use

   Tags

   GFP and Myc

   Expression

   Yeast

   Mutation

   Promoter

   PHO5

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

6. p426-Cup1p-FUS-FusionRed

   Plasmid

   #188393

   Purpose

   Cu2+-dependent expression of N-terminal FUS fused with red fluorescent protein in yeast cells

   Depositor

   Michihiko Kataoka

   Article

   Utsumi et al PLoS One. 2023 Apr 13;18(4):e0283002. do

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   FusionRed

   Expression

   Yeast

   Mutation

   Promoter

   CUP1 promoter

   Available since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAG413GAL-ccdB-HA

   Plasmid

   #14237

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   3HA

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 29, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pPAeBlueHPV16LR

   Plasmid

   #185893

   Purpose

   2-micron plasmid of AeBlue + HPV16 L1 expression module at RPL25 locus

   Depositor

   Claudia Vickers

   Insert

   PALD6>AeBlue>TPGK1- PSe.GAL2> HPV16-L1_C-6*H > TRPL41B
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pYES2.1-cerMET10

   Plasmid

   #107440

   Purpose

   Overexpression of MET fusion protein in S. cerevisiae

   Depositor

   Clare O'Connor

   Article

   Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

   Insert

   MET10 (MET10 Budding Yeast)
   

   Use

   Tags

   V5-His

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 413-Met25 Kar2 TorsinA GFP

    Plasmid

    #32119

    Depositor

    Susan Lindquist

    Article

    Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

    Insert

    TorsinA (TOR1A Human)
    

    Use

    Tags

    GFP and Kar2

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pHES841

    Plasmid

    #89194

    Purpose

    Expresses YFP under control of synthetic promoter containing Zif268 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    pZ-YFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pZ

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBS4

    Plasmid

    #83793

    Purpose

    Useful for tagging yeast proteins with C-terminal CFP tag; hygromycin-B selection

    Depositor

    Eric Muller

    Article

    Hailey et al Methods Enzymol. 2002;351:34-49.

    Insert

    CFP
    

    Use

    Tags

    CFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDS272

    Plasmid

    #34975

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2-GFP-LOVpep (MID2 A. sativa, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    T406A, T407A

    Promoter

    TEF

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNSU299_SEO1

    Plasmid

    #166076

    Purpose

    Plasmid for constituive spCas9 and tet-inducible SEO1 targeting sgRNA expression for double stranded break formation in yeast

    Depositor

    Rebecca Burgess

    Insert

    SEO1 (SEO1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Tet-inducible

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pUCdeltaSS-ERE

    Plasmid

    #108217

    Purpose

    Yeast reporter gene with estrogen response element (ERE) upstream of CYC1 promoter driving lacZ expression

    Depositor

    Didier Picard, Keith Yamamoto

    Article

    Picard et al Nature 1990 Nov 8;348(6297):166-8.

    Insert

    ERE-CYC1-lacZ
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CYC1

    Available since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAV0714

    Plasmid

    #133479

    Purpose

    Expression in fission yeast from promoter pact1, integrated at ura4 locus

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(act1)-sfGFP-terminator(ScCYC1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pNXRVj-HACre

    Plasmid

    #78407

    Purpose

    constitutive Cre expression vector

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Inserts

    3xHA-nls-Cre
    aur1R (aur1 Fission Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Gly 240 to Cys

    Promoter

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pWS484

    Plasmid

    #123032

    Purpose

    LexA UAS

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    LexO (2x) [2a]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pWS483

    Plasmid

    #123031

    Purpose

    LexA UAS

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    LexO (3x) [2a]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pWS482

    Plasmid

    #123030

    Purpose

    LexA UAS

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    LexO (4x) [2a]
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge