8,086 results

1. pPt_Cp

   Plasmid

   #206855

   Purpose

   A plasmid containing the whole P. tricornutum chloroplast genome, cloned with a pCC1BAC-based backbone. The marker URA3 was integrated into the genome.

   Depositor

   Bogumil Karas

   Article

   Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pICE TRP1

   Plasmid

   #122881

   Purpose

   TRP1 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG305GAL-ccdB-EGFP

   Plasmid

   #14185

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pREMI-Z

   Plasmid

   #59527

   Purpose

   Zeocin-resistance plasmid for insertional mutagenesis.

   Depositor

   Benjamin Glick

   Article

   Mukaiyama et al Genes Cells. 2002 Jan;7(1):75-90.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available Since

   Oct. 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. NM R(delta) 2-5 GFP

   Plasmid

   #1209

   Depositor

   Susan Lindquist

   Article

   Liu et al Nature 1999 Aug 5;400(6744):573-6.

   Insert

   sup35 NM domain (SUP35 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   last four repeats of sup35 N domain deleted

   Promoter

   Available Since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS-SKII-3XHA-fSNAP-Kan

   Plasmid

   #188918

   Purpose

   Integration cassette to create C-terminal fusions for fluorescent tagging

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   3XHA-fSNAP-Kan
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFA6a-link-yoLSS-mKate2-CaURA3

   Plasmid

   #44886

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with CaUra3 selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoLSS-mKate2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Oct. 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS406-PHO5-GFP-2xOsh2 PH domain [short]

   Plasmid

   #58829

   Purpose

   Expresses GFP-Tagged Osh2 PH domain in yeast

   Depositor

   Tim Levine

   Article

   Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

   Insert

   2xOsh2 PH domain [short] (OSH2 Budding Yeast)
   

   Use

   Tags

   GFP and Myc

   Expression

   Yeast

   Mutation

   Promoter

   PHO5

   Available Since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pAG414GAL-ccdB-HA

   Plasmid

   #14239

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   3HA

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 29, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pACT4-DM

    Plasmid

    #111233

    Purpose

    yeast expression vector

    Depositor

    Ulrich Stelzl

    Article

    Worseck et al Methods Mol Biol. 2012;812:63-87. doi: 1

    Insert

    ccdB-cassette
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDS272

    Plasmid

    #34975

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2-GFP-LOVpep (MID2 A. sativa, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    T406A, T407A

    Promoter

    TEF

    Available Since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pL90-Nat-2xHO

    Plasmid

    #139069

    Purpose

    CRISPR/Cas9 engineering of the HO endonuclease gene with nourseothricin selection

    Depositor

    Gianni Liti

    Article

    Barre et al Genome Res. 2020 Apr 10. pii: gr.253351.

    Insert

    Two guide RNAs targeting the HO endonuclease
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    TDH3

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOAD

    Plasmid

    #196825

    Purpose

    Yeast two-hybrid ; Gal4 activation domain

    Depositor

    Stan Fields

    Article

    Uetz et al Nature. 2000 Feb 10;403(6770):623-7. doi

    Type

    Empty backbone

    Use

    Tags

    Gal4 Activation domain

    Expression

    Yeast

    Mutation

    Promoter

    ADH

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHES841

    Plasmid

    #89194

    Purpose

    Expresses YFP under control of synthetic promoter containing Zif268 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    pZ-YFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pZ

    Available Since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. 413-Met25 Kar2 TorsinA GFP

    Plasmid

    #32119

    Depositor

    Susan Lindquist

    Article

    Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

    Insert

    TorsinA (TOR1A Human)
    

    Use

    Tags

    GFP and Kar2

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pAG306GPD-ccdB-EGFP

    Plasmid

    #14188

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAG413GAL-ccdB-HA

    Plasmid

    #14237

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBS4

    Plasmid

    #83793

    Purpose

    Useful for tagging yeast proteins with C-terminal CFP tag; hygromycin-B selection

    Depositor

    Eric Muller

    Article

    Hailey et al Methods Enzymol. 2002;351:34-49.

    Insert

    CFP
    

    Use

    Tags

    CFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pICE LYS2

    Plasmid

    #122879

    Purpose

    LYS2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. NAT-TET-OFF -MET - 3xHA

    Plasmid

    #182526

    Purpose

    Doxycycline-induced transcriptional regulation and 3xHA N-terminal tagging.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    7 Copies of TetO Box
    

    Use

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge