8,418 results

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G31, Cdc20-AID tagging

Plasmid

#130269

Purpose

Tagging the Saccharomyces cerevisiae Cdc20 gene with a auxin-degron tag, via homologous recombination

Depositor

Matthias Heinemann

Publication

Ozsezen et al Cell Syst. 2019 Oct 4. pii: S2405-4712(1

Insert

Cdc20/AID(71-114)-tCYC1-NatMX (CDC20 Synthetic, Budding Yeast)

Tags

AID(71-114)

Expression

Yeast

Promoter

Native promoter

Available since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pJW1660

Plasmid

#112034

Purpose

C-terminal tag with AID*(AtIAA17_71-114)-HA marked with LEU

Depositor

Jonathan Weissman

Publication

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

IAA17

Tags

HA

Expression

Yeast

Mutation

only amino acids 71-114

Promoter

None

Available since

Aug. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pMJA181

Plasmid

#128539

Purpose

Expresses anti-Prunus dulcis scFv Ab+BirA tag

Depositor

Marcos Alcocer

Publication

de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

Insert

scFv Ab clone PD1F6 plus BirA tag

Expression

Yeast

Promoter

AOX1

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pMJA179

Plasmid

#128537

Purpose

Expresses anti-Prunus dulcis scFv Ab

Depositor

Marcos Alcocer

Publication

de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

Insert

scFv Ab clone PD1F6

Expression

Yeast

Promoter

AOX1

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pMJA103

Plasmid

#128519

Purpose

Expresses human amyloid Lysozyme in Pichia pastoris

Depositor

Marcos Alcocer

Publication

Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

Insert

Artificial gene for human lysozyme D67H (LYZ Human)

Expression

Yeast

Promoter

AOX1

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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VEGF binding scFv G6

Plasmid

#111716

Purpose

Yeast Surface Display of G6 as a reference starting point for design

Depositor

Sarel Fleishman

Publication

Warszawski et al PLoS Comput Biol. 2019 Aug 23;15(8):e100

Insert

G6

Tags

cmyc tag

Expression

Yeast

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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Destination Vector

Plasmid

#125754

Purpose

Destination vector for assembling gRNA fragments in the second round of assembly.

Depositor

Rodrigo Ledesma-Amaro

Publication

McCarty et al ACS Synth Biol. 2019 Apr 2. doi: 10.1021

Insert

pTDH3-GFPdropout-tTDH1

Use

Synthetic Biology

Expression

Yeast

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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YIp TRP1-HphMx

Plasmid

#122922

Purpose

TRP1-HphMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

July 4, 2019

Availability

Academic Institutions and Nonprofits only

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pICE LYS2

Plasmid

#122879

Purpose

LYS2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

Depositor

Randy Hampton

Publication

Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

Type

Empty backbone

Use

Yeast recombination cloning

Expression

Yeast

Available since

May 25, 2019

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-GgKDELR2

Plasmid

#123618

Purpose

Expresses WT GgKDELR2 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Publication

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Tags

TEV-GFP-His

Expression

Yeast

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHES553 [prGal1-RAS2(KAN) pnh604]

Plasmid

#103846

Purpose

Yeast Expression Plasmid

Depositor

Hana El-Samad

Publication

Stewart-Ornstein et al Mol Biol Cell. 2017 Jan 1;28(1):221-227.

Insert

pGal1-Ras2(KAN)

Expression

Yeast

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pPICZ-mouseGIRK2-eGFP-StII-6xHis

Plasmid

#124277

Purpose

Expresses mouse GIRK2 as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tag

Depositor

Arthur Laganowsky

Publication

Liu et al Nat Commun. 2019 Mar 22;10(1):1352. doi:

Insert

GIRK2 (Kcnj6 Mouse)

Tags

eGFP-StreptagII-6xHis

Expression

Yeast

Promoter

AOX1

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pWS1806

Plasmid

#123047

Purpose

Melatonin GPCR

Depositor

Tom Ellis

Publication

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

MTNR1A [3]

Expression

Yeast

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKar2-msGFP-HDEL

Plasmid

#117668

Purpose

Localisation of Endoplasmic Reticulum

Depositor

Pau Ferrer, Benjamin Glick

Publication

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pKar2-msGFP-HDEL

Expression

Yeast

Mutation

Contains the Kar2 promoter and signal sequence fr…

Promoter

pKar2

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW mKate2-T2A-mTurquoise2

Plasmid

#118426

Purpose

Produces equimolar expression of mKate2 and mTq2

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

mKate2-T2A-mTq2

Expression

Yeast

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW mScarlet-T2A-mTurquoise2

Plasmid

#118431

Purpose

Produces equimolar expression of mScarlet and mTq2

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

mScarlet-T2A-mTurquoise2

Expression

Yeast

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pREF-GFP kan

Plasmid

#122061

Purpose

reporter plasmid for termination and terminator override to express GFP. Marked with URA3

Depositor

Daniel Reines

Publication

Reines et al Methods. 2019 Jan 4. pii: S1046-2023(18)

Inserts

intergenic terminator from yeast IMD2
green fluorescent protein

Expression

Yeast

Promoter

GAL1 and GAL1 comes in pYES2

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAH233

Plasmid

#121144

Purpose

Expression of gRNA with BbsI placeholder for target cloning

Depositor

Katsunori Tanaka

Publication

Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

Insert

gRNA cassette

Use

CRISPR

Expression

Yeast

Promoter

S.pombe rrk1

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pMEG_BB3_YL001

Plasmid

#117825

Purpose

Overexpession plasmid of glycerol kinase (GUT1) in Y. lipolytica

Depositor

Michael Sauer

Publication

Egermeier et al FEMS Microbiol Lett. 2019 Jan 29. pii: 5

Insert

YlpTEF_YlGUT1_YlCyc1TT

Expression

Bacterial and Yeast

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pRS306-pHIS3-2xGFP-tURA3

Plasmid

#116936

Purpose

Yeast integrative vector for the expression of 2xGFP

Depositor

Liam Holt

Publication

Delarue et al Cell. 2018 Jul 12;174(2):338-349.e20. do

Insert

2xGFP

Expression

Yeast

Promoter

HIS3

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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