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8,173 results

Showing: 5751 - 5800 of 8173 results

  1. pCAS-mak10 gRNA

    Plasmid
    #160385
    Purpose
    Expresses guide RNA for targeting MAK10 in yeast
    Depositor
    John McCusker
    Insert
    mak10 gRNA
    Expression
    Yeast
    Promoter
    RNA pol III promoter (tRNA-Tyr)
    Available Since
    Feb. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    160385-plasmid-map-sequence-id-384166

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  8. trp1D-proLYS2-GFP-PKCdelta(C1a+C1b)-NAT

    Plasmid
    #184774
    Purpose
    Marker for DAG. Yeast expression of mouse PKCdelta under LSY2 promoter. Uses antibiotic resistance marker natMX6. Replaces endogenous TRP1 upon genome integration, leading to trp1D.
    Depositor
    Zhiping Xie
    Article
    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10
    Insert
    proLYS2-GFP-PKCdelta(C1a+C1b) (Prkcd Mouse, Budding Yeast)
    Tags
    EGFP
    Expression
    Yeast
    Available Since
    Feb. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    184774-plasmid-map-sequence-id-382498

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  10. P2061

    Plasmid
    #186299
    Purpose
    Expresses LexA-HBD-B112 under the control of pAct1 and Cas9 under the control of LexA-HBD-B112+Beta-estradiol inducible promoter
    Depositor
    Joerg Stelling
    Article
    Azizoglu et al Curr Protoc. 2023 Jan;3(1):e647. doi: 10
    Inserts
    LexA-HBD-B112
    Cas9
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial and Yeast
    Promoter
    2xLexop-minimalCyc1 and PACT1
    Available Since
    Feb. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    186299-plasmid-map-sequence-id-367190

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  27. C-SWAT GFP CL1 DAmP

    Plasmid
    #182513
    Purpose
    C-SWAT compatible vector for C-terminal tagging with GFP CL1 followed by the DAmP cassette. The stop codon after the CL1 degron is immediately followed by the Hygromycin B (Hph) resistance cassette.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    CL1 and DAmP
    Tags
    GFP
    Expression
    Yeast
    Available Since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    182513-plasmid-map-sequence-id-358273

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  33. pAG25-1199 cDNA

    Plasmid
    #160379
    Purpose
    N1199 narnavirus cDNA
    Depositor
    John McCusker
    Insert
    N1199
    Expression
    Yeast
    Promoter
    NA
    Available Since
    Dec. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    160379-plasmid-map-sequence-id-314410

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  34. PJD1223

    Plasmid
    #160390
    Purpose
    Encodes L-A cDNA for curing L-A virus and Parent for pJD1223-NAT
    Depositor
    John McCusker
    Insert
    L-A Gag-Pol
    Expression
    Yeast
    Promoter
    TRP1
    Available Since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    160390-plasmid-map-sequence-id-314529

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  35. N1199 launching

    Plasmid
    #160386
    Purpose
    Expression of N1199 narnavirus ORF under PGK1 promoter with a 3' HDV ribozyme sequence
    Depositor
    John McCusker
    Insert
    N1199
    Expression
    Yeast
    Promoter
    PGK1
    Available Since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    160386-plasmid-map-sequence-id-314521

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  36. pGAL1-VP1 (LEU2)

    Plasmid
    #182480
    Purpose
    Yeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains leucine auxotrophic marker (K. lactis LEU2).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Insert
    deltaVP1
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL1
    Available Since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182480-plasmid-map-sequence-id-366129

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  37. deltaVP1d63C + VP2C-GFPdeg

    Plasmid
    #182481
    Purpose
    YIp for expressing Murine polyomavirus deltaVP1 with the 63 C-ter residues truncated (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-GFPdeg
    deltaVP1d63C
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL1 and GAL10
    Available Since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182481-plasmid-map-sequence-id-360432

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  38. mRuby3 only VLP

    Plasmid
    #182429
    Purpose
    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and mRuby3 tagged with VP2C (GAL10 promoter). mRuby3 was codon-optimised for E. coli. Contains uracil marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-mRuby3
    deltaVP1
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    GAL1 and GAL10
    Available Since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182429-plasmid-map-sequence-id-360111

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  39. Linked mRuby3 VLP

    Plasmid
    #182430
    Purpose
    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-GFP-mRuby3 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-GFP-mRuby3
    deltaVP1
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    GAL1 and GAL10
    Available Since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182430-plasmid-map-sequence-id-360112

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  40. Coexpressed mRuby3 VLP

    Plasmid
    #182431
    Purpose
    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-GFP (GAL10 promoter), and VP2C-mRuby3 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-GFP
    deltaVP1
    VP2C-mRuby3
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    GAL1, GAL10, and GAL7
    Available Since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182431-plasmid-map-sequence-id-360113

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  41. Linked VLP NES

    Plasmid
    #182432
    Purpose
    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-FPPS-NES
    deltaVP1
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL1 and GAL10
    Available Since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182432-plasmid-map-sequence-id-360114

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  42. Coexpressed VLP NES

    Plasmid
    #182433
    Purpose
    Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20 (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3)
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-FPPS
    deltaVP1
    VP2C-NES
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL1, GAL10, and GAL7
    Available Since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182433-plasmid-map-sequence-id-360116

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  43. Linked VLP NES (M)

    Plasmid
    #182434
    Purpose
    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-FPPS(M)-NES
    deltaVP1
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL1 and GAL10
    Available Since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182434-plasmid-map-sequence-id-360117

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  44. Coexpressed VLP NES (M)

    Plasmid
    #182435
    Purpose
    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96W-N127W) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-FPPS(M)
    deltaVP1
    VP2C-NES
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL1, GAL10, and GAL7
    Available Since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182435-plasmid-map-sequence-id-360119

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  45. Linked Free NES (M)

    Plasmid
    #182436
    Purpose
    Yeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Insert
    FPPS(M)-NES
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL10
    Available Since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182436-plasmid-map-sequence-id-360120

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  46. Coexpressed Free NES (M)

    Plasmid
    #182437
    Purpose
    Yeast integrative plasmid for expressing ERG20(F96W-N127W) (GAL10 promoter) and AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    FPPS(M)
    NES
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL10 and GAL7
    Available Since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182437-plasmid-map-sequence-id-360121

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  47. Linked VLP NES (G)

    Plasmid
    #182476
    Purpose
    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96C)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-FPPS(G)-NES
    deltaVP1
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL1 and GAL10
    Available Since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182476-plasmid-map-sequence-id-360123

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  48. Coexpressed VLP NES (G)

    Plasmid
    #182477
    Purpose
    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96C) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al bioRxiv 2022.11.24.517869
    Inserts
    VP2C-FPPS(G)
    deltaVP1
    VP2C-NES
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL1, GAL10, and GAL7
    Available Since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    182477-plasmid-map-sequence-id-360429

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Showing: 5751 - 5800 of 8173 results