7,941 results
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Plasmid#178994PurposeSp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitURA3DepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK09 pCAS-Tyr-[gRNA: BsaI GFP dropout] (BsmBI-flanked URA3, AmpR)
Plasmid#178993PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = URA3DepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK08 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitLEU2,AmpR)
Plasmid#178992PurposeSp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitLEU2DepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK07 pCAS-Tyr-[gRNA: BsaI GFP dropout] (LEU2,AmpR)
Plasmid#178991PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = LEU2DepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK06 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitNatR,AmpR)
Plasmid#178990PurposeSp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitNourseothrycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEDJ506
Plasmid#177274PurposeADH1t:HIS3_23∆29-XII-5 :<-PGK1pDepositorInsertADH1t:HIS3_23∆29-XII-5 :<-PGK1p
UseTagsExpressionBacterial and YeastMutationPromoterPGK1Available sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pL-mWasabi
Plasmid#176552PurposeExpression of mWasabi for auxotrophic selection in the absence of leucineDepositorInsertmWasabi
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM-eCFP
Plasmid#176558PurposeExpression of eCFP for auxotrophic selection in the absence of methionineDepositorInserteCFP
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH-TagRFP657
Plasmid#176560PurposeExpression of TagRFP657 for auxotrophic selection in the absence of lysineDepositorInsertTagRFP657
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM-TagRFP657
Plasmid#176563PurposeExpression of TagRFP657 for auxotrophic selection in the absence of methionineDepositorInsertTagRFP657
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGB-Co-NF-kB
Plasmid#180206PurposeExpression GAL4-fusion of Co-NF-kB in yeastDepositorInsertCo-NF-kB
UseTagsGAL4 (1-147) fusionExpressionYeastMutationaa 2-1224PromoterAdhIAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGB-As-NF-kB2
Plasmid#180210PurposeExpresses GAL4-fusion protein of Acanthoeca spectabilis NF-kB2 protein in yeastDepositorInsertAs-NF-kB2
UseTagsGAL4 (aa 1-147) fusion proteinExpressionYeastMutationaa 2-502PromoterAdhIAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJL32
Plasmid#174066PurposeEncodes for CIB1 fused to VP16 in S. cerevisiae. Part of optogenetic system.DepositorInsertpGal1-LacO-3NLS-CIB1-VP16-tADH1
UseTagsFLAGExpressionYeastMutationPromoterpGal1Available sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
GLuc VLP
Plasmid#182213PurposeYIp for expressing MPyV deltaVP1 (GAL1 promoter) and Gaussia luciferase tagged with VP2C (GAL10 promoter).DepositorInsertsVP2C-GLuc
Murine polyomavirus deltaVP1 (NLS deletion mutant)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
Akaluc VLP
Plasmid#182212PurposeYIp for expressing MPyV deltaVP1 (GAL1 promoter) and Akaluc tagged with VP2C (GAL10 promoter).DepositorInsertsVP2C-Akaluc
Murine polyomavirus deltaVP1 (NLS deletion mutant)
UseSynthetic Biology; Tem imagingTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGB-Co-Cterm
Plasmid#180208PurposeExpresses GAL4 fusion of Capsaspora NF-kB C-terminal sequences in yeastDepositorInsertCo-RHD
UseTagsGAL4 (1-147) fusionExpressionYeastMutationaa 542-1224PromoterAdhIAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGB-As-NF-kB3
Plasmid#180211PurposeExpresses GAL4-fusion protein of Acanthoeca spectabilis NF-kB3 protein in yeastDepositorInsertAs-NF-kB3
UseTagsGAL4 (aa 1-147) fusion proteinExpressionYeastMutationaa 2-412PromoterAdhIAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV491
Plasmid#177699PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.DepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastMutationPromoterRNR2p (Debaryomyces hansenii ) and TEF1p (Ashbya …Available sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV495
Plasmid#177702PurposePlasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hanseniiDepositorInsertsCas9
Nat
sgRNA Targeting ADE2 locus in D.hansenii: AGCTAaGCAGATTAAtGCAT
UseCRISPRTagsSV40ExpressionBacterial and YeastMutationPromoterRNR2p (Debaryomyces hansenii ), SRC1p (Debaryomyc…Available sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV488
Plasmid#177696PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast speciesDepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastMutationPromoterTDH3p (Candida lusitaniae) and TEF1p (Ashbya goss…Available sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSJ982
Plasmid#176622PurposeExpress yeast Ub-MPGLW-Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.DepositorInsertUb-MPGLW-Fbp1
UseTagsUbiquitin N-terminal fusion and triple flag tag C…ExpressionYeastMutationChanged first 4 N-terminal amino acid residues of…PromoterTDH3tc3Available sinceMarch 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
RP51A-No5'ss-NoBS-NoPseudoBS
Plasmid#100987PurposeRP51A with T7 promoter in HindIII/EcoRI sites of pUC18. No 5'ss, No BS, No pseudo BSDepositorInsertRP51A (RPS17A Budding Yeast)
UseTagsExpressionBacterial and YeastMutation5'ss mut to C/ATACCT BS mut to GTTAGTG, pseu…PromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rp51a Hyperstabilized 5'SS
Plasmid#100988PurposepUC18 Hyperstabilized Rp51a 5'SS between HindIII and EcoRI; ATG/GTATGT -> AAG/GTAAGTDepositorInsertRP51A (RPS17A Budding Yeast)
UseTagsExpressionBacterial and YeastMutation5'ss mut ATG/GTATGT -> AAG/GTAAGTPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rp51a_10bp 5'SS_No BP or pBP_pUC18
Plasmid#100989PurposeRp51a with a tGGTAtGTta->AGGTAAGTAT 5'SS mutant with potential to form 10bps with the U1 snRNA. Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutationDepositorInsertRP51A (RPS17A Budding Yeast)
UseTagsExpressionBacterial and YeastMutationtGGTAtGTta->AGGTAAGTAT 5'SS mutant, TACTA…PromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rp51a_SUS1 5'ss_No_BP_pBP-pUC18
Plasmid#100990PurposeContains model Rp51a with a GGTAtGT->tGTAtGa 5'SS mutant (SUS1 5'SS sequence). Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutationDepositorInsertRP51A (RPS17A Budding Yeast)
UseTagsExpressionBacterial and YeastMutationGGTAtGT->tGTAtGa 5'SS mutant, TACTAAC->…PromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rp51a_SUS1 5'SS
Plasmid#100991PurposeContains model Rp51a with a GGTAtGT->tGTAtGa 5'SS mutant (SUS1 5'SS sequence).DepositorInsertRP51A (RPS17A Budding Yeast)
UseTagsExpressionBacterial and YeastMutationGGTAtGT->tGTAtGa 5'SS mutantPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rp51a_No_BP-pUC18
Plasmid#100992PurposeContains model Rp51a with a TACTAAC->GTTAGTG BP mutataionDepositorInsertRP51A (RPS17A Budding Yeast)
UseTagsExpressionBacterial and YeastMutationTACTAAC->GTTAGTG BP mutataionPromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
RP51A-NoBS-NoPseudoBS
Plasmid#100986PurposeRP51A w/ T7 promoter in HindIII/EcoRI sites of pUC18. Has WT 5'ss but mutant BS and no pseudo BSDepositorInsertRP51A (RPS17A Budding Yeast)
UseTagsExpressionBacterial and YeastMutationBS mutated to GTTAGTG, pseudo BS mutated to GTTTG…PromoterAvailable sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSSa31
Plasmid#179504PurposeTet-on genome insertion systemDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterTet-HOP1 (p98CGH)Available sinceFeb. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSSa32
Plasmid#179505PurposeTet-on genome insertion systemDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterTet-HOP1 (p99CGH)Available sinceFeb. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFBP-2_6m.mutant
Plasmid#162842PurposeYeast expression of the mutant #2_6m for the RNA-based sensor of fructose-1,6-bisphosphate #2_6DepositorInsert2_6m_RNA-device
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pML104-natR412
Plasmid#162042PurposeExpresses Cas9 and contains natR412 guide which targets the natR geneDepositorInsertnatR412 guide
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pML107-natR854
Plasmid#162043PurposeExpresses Cas9 and contains natR854 guide which targets the natR geneDepositorInsertnatR854 guide
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN513
Plasmid#175365PurposeYeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. Low copy, HIS3-selected.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN514
Plasmid#175366PurposeYeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. Low copy, TRP1-selected.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN515
Plasmid#175367PurposeYeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. Low copy, LEU2-selected.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN516
Plasmid#175368PurposeYeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. Low copy, URA3-selected.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN523
Plasmid#175369PurposeYeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, HIS3-selected.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN524
Plasmid#175370PurposeYeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, TRP1-selected.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN525
Plasmid#175371PurposeYeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, LEU2-selected.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN526
Plasmid#175372PurposeYeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, URA3-selected.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN613
Plasmid#175373PurposeYeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. Low copy, HIS3-selected.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN614
Plasmid#175374PurposeYeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. Low copy, TRP1-selected.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN615
Plasmid#175375PurposeYeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. Low copy, LEU2-selected.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN616
Plasmid#175376PurposeYeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. Low copy, URA3-selected.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN623
Plasmid#175377PurposeYeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, HIS3-selected.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN624
Plasmid#175378PurposeYeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, TRP1-selected.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN625
Plasmid#175379PurposeYeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, LEU2-selected.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN626
Plasmid#175380PurposeYeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, URA3-selected.DepositorTypeEmpty backboneUseCre/LoxTagsExpressionYeastMutationPromoterAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
p406 – TDH3-ER-FlucDM-GFP11-Ura
Plasmid#177731PurposeDM Firefly luciferase reporter fused to a KAR2 signal sequence for ER targeting and HDEL for ER retention with an HA tag and the eleventh β-strand of GFP added to the C-terminus in yeast plasmid withDepositorInsertFlucDM
UseTagsGFP11, HA, HDEL, and KAR2 Signal SequenceExpressionBacterial and YeastMutationR188Q, R261Q (Destabilized Mutant)PromoterAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only