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8,173 results

Showing: 5801 - 5850 of 8173 results

  7. pIAeBlueHPV16LR

    Plasmid
    #185894
    Purpose
    In vivo gene amplification (HamAmp) of AeBlue + HPV16 L1 expression module at RPL25 locus
    Depositor
    Claudia Vickers
    Insert
    PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-TRPL25(Arm3)-ARS305-PALD6>EforRed>TPGK1-PSe.GAL2>HPV16-L1_C-6*H>TRPL41B-PBTS1>RPL25(partial;Arm2)
    Expression
    Yeast
    Mutation
    None
    Available Since
    Nov. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    185894-plasmid-map-sequence-id-374392

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  8. Coexpressed Free NES

    Plasmid
    #182482
    Purpose
    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and nerolidol synthase from Actinidia chinensis (AcNES1; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Inserts
    FPPS
    NES
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL10 and GAL7
    Available Since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182482-plasmid-map-sequence-id-360445

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  9. Coexpressed Free PcPTS

    Plasmid
    #182483
    Purpose
    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and patchoulol synthase from Pogostemon cablin (PcPTS; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Inserts
    FPPS
    PTS
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL10 and GAL7
    Available Since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182483-plasmid-map-sequence-id-360444

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  10. Coexpressed Free ClLIS1 (M)

    Plasmid
    #182484
    Purpose
    Yeast integrative plasmid for expressing ERG20(F96W-N127W) (GAL10 promoter) and limonene synthase from Citrus limon (ClLIS1; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Inserts
    FPPS(M)
    LS
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL10 and GAL7
    Available Since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182484-plasmid-map-sequence-id-360440

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  12. Linked Free (PT)4P

    Plasmid
    #182490
    Purpose
    Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence PTPTPTPTP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Insert
    FPPS-NES
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL10
    Available Since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182490-plasmid-map-sequence-id-360448

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  13. Coexpressed Free deadFPPS-NES

    Plasmid
    #182492
    Purpose
    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein deadFPPS-AcNES1 (GAL7 promoter). deadFPPS is ERG20(K197G-K254A), an inactive mutant of ERG20.
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Inserts
    FPPS
    deadFPPS-NES
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL10 and GAL7
    Available Since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182492-plasmid-map-sequence-id-360446

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  14. pSCL.040

    Plasmid
    #184974
    Purpose
    Test effect of extending a1/a2 on ADE2 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v2
    Expression
    Yeast
    Mutation
    ADE2 donor P272stop, a1/a2 length extended to 27 …
    Promoter
    Gal7
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184974-plasmid-map-sequence-id-376705

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  19. pSCL.109

    Plasmid
    #184978
    Purpose
    Test effect of extending a1/a2 on LYP1 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, LYP1 E27X, a1/a2 length: 27 v1
    Expression
    Yeast
    Mutation
    LYP1 donor E27stop, a1/a2 length extended to 27 bp
    Promoter
    Gal7
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184978-plasmid-map-sequence-id-376679

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  20. pSCL.107

    Plasmid
    #184976
    Purpose
    Test effect of extending a1/a2 on CAN1 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, CAN1 G444X, a1/a2 length: 27 v1
    Expression
    Yeast
    Mutation
    CAN1 donor G444stop, a1/a2 length extended to 27 …
    Promoter
    Gal7
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184976-plasmid-map-sequence-id-376676

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  24. pSCL.111

    Plasmid
    #184980
    Purpose
    Test effect of extending a1/a2 on TRP2 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 27 v1
    Expression
    Yeast
    Mutation
    TRP2 donor E64stop, a1/a2 length extended to 27 bp
    Promoter
    Gal7
    Available Since
    Nov. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    184980-plasmid-map-sequence-id-376669

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  33. pSCL.113

    Plasmid
    #184982
    Purpose
    Test effect of extending a1/a2 on FAA1 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 27 v1
    Expression
    Yeast
    Mutation
    FAA1 donor P233stop, a1/a2 length extended to 27 …
    Promoter
    Gal7
    Available Since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    184982-plasmid-map-sequence-id-376532

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  36. pSCL.039

    Plasmid
    #184973
    Purpose
    Test effect of extending a1/a2 on ADE2 editing rates in yeast
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v1
    Expression
    Yeast
    Mutation
    ADE2 donor P272stop, a1/a2 length extended to 27 …
    Promoter
    Gal7
    Available Since
    Nov. 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    184973-plasmid-map-sequence-id-376529

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  39. pH-mWasabi

    Plasmid
    #176551
    Purpose
    Expression of mWasabi for auxotrophic selection in the absence of histidine
    Depositor
    Markus Ralser
    Insert
    mWasabi
    Expression
    Yeast
    Promoter
    TDH1(GAP)
    Available Since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    176551-plasmid-map-sequence-id-350293

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  40. pU-mWasabi

    Plasmid
    #176553
    Purpose
    Expression of mWasabi for auxotrophic selection in the absence of uracil
    Depositor
    Markus Ralser
    Insert
    mWasabi
    Expression
    Yeast
    Promoter
    TDH1(GAP)
    Available Since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    176553-plasmid-map-sequence-id-350305

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  41. pM-mWasabi

    Plasmid
    #176554
    Purpose
    Expression of mWasabi for auxotrophic selection in the absence of methionine
    Depositor
    Markus Ralser
    Insert
    mWasabi
    Expression
    Yeast
    Promoter
    TDH1(GAP)
    Available Since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    176554-plasmid-map-sequence-id-350308

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  42. pU-TagRFP657

    Plasmid
    #176562
    Purpose
    Expression of TagRFP657 for auxotrophic selection in the absence of uracil
    Depositor
    Markus Ralser
    Insert
    TagRFP657
    Expression
    Yeast
    Promoter
    TDH1(GAP)
    Available Since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    176562-plasmid-map-sequence-id-350316

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  43. pU-YLL058W-lt

    Plasmid
    #176564
    Purpose
    Expression of YLL058W for Lanchea thermotolerans for auxotrophic selection in the absence of uracil
    Depositor
    Markus Ralser
    Insert
    YLL058W-lt
    Expression
    Yeast
    Promoter
    TDH1(GAP)
    Available Since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    176564-plasmid-map-sequence-id-350304

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  44. pU-YLL058W-zr

    Plasmid
    #176565
    Purpose
    Expression of YLL058W from Zygosaccharomyces rouxii for auxotrophic selection in the absence of uracil
    Depositor
    Markus Ralser
    Insert
    YLL058W-zr
    Expression
    Yeast
    Mutation
    Codon optimised for expression in S.cerevisae
    Promoter
    TDH1(GAP)
    Available Since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    176565-plasmid-map-sequence-id-350306

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  45. pH-Sapphire

    Plasmid
    #176566
    Purpose
    Expression of Sapphire for auxotrophic selection in the absence of lysine
    Depositor
    Markus Ralser
    Insert
    yETSapphire
    Expression
    Yeast
    Promoter
    TDH1(GAP)
    Available Since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    176566-plasmid-map-sequence-id-350974

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  46. pU-Sapphire

    Plasmid
    #176567
    Purpose
    Expression of Sapphire for auxotrophic selection in the absence of uracil
    Depositor
    Markus Ralser
    Insert
    yETSapphire
    Expression
    Yeast
    Promoter
    TDH1(GAP)
    Available Since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    176567-plasmid-map-sequence-id-353606

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  47. pM-Sapphire

    Plasmid
    #176569
    Purpose
    Expression of Sapphire for auxotrophic selection in the absence of methionine
    Depositor
    Markus Ralser
    Insert
    yETSapphire
    Expression
    Yeast
    Promoter
    TDH1(GAP)
    Available Since
    Oct. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    176569-plasmid-map-sequence-id-350302

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  49. pIFEX-A2aGH

    Plasmid
    #160542
    Purpose
    pITY4 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.
    Depositor
    Michelle O'Malley
    Article
    Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:
    Insert
    Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)
    Expression
    Yeast
    Mutation
    N/A
    Available Since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    160542-plasmid-map-sequence-id-317791

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  50. pEFEX1-A2aGH

    Plasmid
    #160543
    Purpose
    pYES2 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.
    Depositor
    Michelle O'Malley
    Article
    Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:
    Insert
    Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)
    Expression
    Yeast
    Mutation
    N/A
    Available Since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    160543-plasmid-map-sequence-id-317760

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Showing: 5801 - 5850 of 8173 results