8,086 results

1. pH-eCFP

   Plasmid

   #176555

   Purpose

   Expression of eCFP for auxotrophic selection in the absence of histidine

   Depositor

   Markus Ralser

   Article

   Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

   Insert

   eCFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH1(GAP)

   Available Since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pL-eCFP

   Plasmid

   #176556

   Purpose

   Expression of eCFP for auxotrophic selection in the absence of leucine

   Depositor

   Markus Ralser

   Article

   Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

   Insert

   eCFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH1(GAP)

   Available Since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pU-eCFP

   Plasmid

   #176557

   Purpose

   Expression of eCFP for auxotrophic selection in the absence of uracil

   Depositor

   Markus Ralser

   Article

   Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

   Insert

   eCFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH1(GAP)

   Available Since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDA200

   Plasmid

   #74248

   Purpose

   pGPD1-dPSTRy(4,3)

   Depositor

   Serge Pelet

   Article

   Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10

   Insert

   pGPD1-dPSTRy(4,3)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pH3FS-PYK1

   Plasmid

   #85781

   Purpose

   N-ICE plasmid pH3FS-PYK1

   Depositor

   Scott Briggs

   Article

   Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

   Insert

   loxP-HphMX-loxP-PYK1p-3FLAG
   

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-kanMX6-P81nmt1-mYFP

   Plasmid

   #105144

   Purpose

   Yeast gene targeting

   Depositor

   Jian-Qiu Wu, Thomas Pollard

   Article

   Wu et al Dev Cell. 2003 Nov;5(5):723-34.

   Insert

   kanMX6-P81nmt1-mYFP
   

   Use

   Tags

   81nmt1-mYFP

   Expression

   Bacterial and Yeast

   Mutation

   A206K

   Promoter

   81nmt1

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. YIPlac204-TC-Sec7-6xDsRed-M1

   Plasmid

   #25448

   Depositor

   Benjamin Glick

   Article

   Losev et al Nature. 2006 Jun 22. 441(7096):1002-6.

   Insert

   Sec7 (SEC7 Budding Yeast)
   

   Use

   Tags

   6xDsRed.M1

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   July 14, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJY3948

   Plasmid

   #113338

   Purpose

   6XHis-tagged 2-pyrone synthase driven by TEF1intron promoter; codon optimized for Yarrowia lipolytica expression

   Depositor

   Suzanne Sandmeyer

   Article

   Yu et al Biotechnol Bioeng. 2018 May 19. doi: 10.

   Insert

   2-pyrone synthase, codon optimized for Y. lipolytica
   

   Use

   Tags

   6XHis

   Expression

   Yeast

   Mutation

   Please see depositor comments

   Promoter

   Available Since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSNAPf-AP

   Plasmid

   #61830

   Purpose

   Encodes SNAP-AP fusion for tagging endogenous target for SNAP-SiMPull

   Depositor

   Aaron Hoskins

   Article

   Rodgers et al RNA. 2015 May;21(5):1031-41. doi: 10.126

   Insert

   SNAP-AP
   

   Use

   Pcr template

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   April 16, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pRS315Nab3

    Plasmid

    #110532

    Purpose

    Expresses yeast NAB3 using the endogenous promoter

    Depositor

    Daniel Reines

    Article

    Loya et al Nucleic Acids Res. 2012 Aug;40(15):7476-

    Insert

    NAB3 (NAB3 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJT42_GalL_BE-PAPAPA

    Plasmid

    #145035

    Purpose

    Expresses BE-PAPAPA in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    BE-PAPAP
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A)

    Promoter

    GalL

    Available Since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GTT-g

    Plasmid

    #81109

    Purpose

    Gene Tagging vector TRP/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Insert

    sfGFP
    

    Use

    Cre/Lox

    Tags

    sfGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pROS14

    Plasmid

    #107928

    Purpose

    LEU2 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y, gRNA-ADE2.Y
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. YIplac211-iGFP-PIK1

    Plasmid

    #105260

    Purpose

    Plasmid for pop-in/pop-out replacement of Pik1 with iGFP-Pik1.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    Pik1 (PIK1 Budding Yeast)
    

    Use

    Tags

    iGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. YIplac211-RPL13A-FKBPx2

    Plasmid

    #104473

    Purpose

    Plasmid for pop-in/pop-out replacement of RPL13A with an FKBPx2-tagged version.

    Depositor

    Benjamin Glick

    Article

    Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

    Insert

    RPL13Ap (RPL13A Budding Yeast)
    

    Use

    Tags

    FKBPx2-HA

    Expression

    Yeast

    Mutation

    includes the last 354 bp of RPL13A only

    Promoter

    Available Since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. POM42-mTFP1

    Plasmid

    #69852

    Purpose

    N-terminal tagging with mTFP1 using URA3 as a selection marker in S. cerevisiae.

    Depositor

    Liza Pon

    Article

    Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

    Insert

    mTFP1 minus start and stop codon
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MtPT4

    Plasmid

    #117096

    Purpose

    protein expression in yeast

    Depositor

    Geoffrey Chang

    Insert

    MtPT4
    

    Use

    Tags

    His

    Expression

    Yeast

    Mutation

    Promoter

    AOX

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GTH-ir

    Plasmid

    #81107

    Purpose

    Gene Tagging vector HIS/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Insert

    tdiRFP
    

    Use

    Cre/Lox

    Tags

    tdiRFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIM572

    Plasmid

    #74640

    Purpose

    alias pFA6a-yTagRFP-T-kanMX6 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagRFP-T and G418 selection marker

    Depositor

    Jiří Hašek

    Article

    Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

    Insert

    yTagRFP-T
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNS3

    Plasmid

    #131770

    Purpose

    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sites

    Depositor

    Jared Toettcher

    Article

    Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

    Insert

    PADH1_FUSN_Fusion Red_Cry2Olig_TACT1
    

    Use

    Integration into δ-sites

    Tags

    Expression

    Yeast

    Mutation

    E490G to make Cry2 into Cry2Olig

    Promoter

    PADH1

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge