8,086 results

1. YN2_1_IL50_HOlocus

   Plasmid

   #194426

   Purpose

   Expresses Cas9 and sgRNA cassettes for targeting the HO locus in yeast Saccharomyces cerevisiae

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   NA

   Promoter

   Available Since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBS-SKII-3XHA-fCLIP-URA

   Plasmid

   #188922

   Purpose

   Integration cassette to create C-terminal fusions for fluorescent tagging

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   3XHA-fCLIP-URA
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. HP-Cas9hGem-Ade2-LEU2-LINEAR

   Plasmid

   #174839

   Purpose

   pRS414 backbone containing LINEAR fragment targeting ADE2 in H. polymorpha

   Depositor

   Zengyi Shao

   Article

   Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

   Insert

   HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   TDH3p

   Available Since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCB32

   Plasmid

   #111911

   Purpose

   Expresses gRNA to target Nte1 locus and has HygR marker

   Depositor

   Yi Tang

   Insert

   gRNA cassette targeting Nte1 locus
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   SNR52p

   Available Since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pICE LEU2

   Plasmid

   #122878

   Purpose

   LEU2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Dec. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. M4757 KanMX::TRP1 Disruptor Converter

   Plasmid

   #51687

   Purpose

   Yeast marker swap plasmid for converting KanMX3 to TRP1

   Depositor

   David Stillman

   Article

   Voth et al Yeast. 2003 Aug;20(11):985-93.

   Insert

   TRP1 (TRP1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   July 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. E2-Crimson-FKBP(M)

   Plasmid

   #85002

   Purpose

   Expresses E2-Crimson-FKBP(M)

   Depositor

   Benjamin Glick

   Article

   Barrero et al Cell Logist. 2016 Jun 24;6(3):e1204848.

   Insert

   E2-Crimson-FKBP(M)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Dec. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGBW-m4046310

   Plasmid

   #149022

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 M (membrane)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 M (membrane) (M Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;MBP

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pGBW-m4046423

   Plasmid

   #148998

   Purpose

   Yeast Expression plasmid for SARS-CoV-2 nsp7

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp7 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable thrombin;MBP

   Expression

   Yeast

   Mutation

   wt

   Promoter

   pTEF1

   Available Since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pAG413GPD-ccdB-HA

    Plasmid

    #14238

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGP8GF-AtTIR1

    Plasmid

    #188427

    Purpose

    Arabidopsis Nuclear Auxin Signaling Receptor TIR1

    Depositor

    Britney Moss

    Article

    Ramos Baez et al Plant Physiol. 2020 Apr;182(4):1713-1722

    Insert

    Auxin Signaling Receptor TIR1 (TIR1 Mustard Weed)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCfB4786

    Plasmid

    #106142

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntC_3, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBS-SKII-URA-GAL1-HALO-GS

    Plasmid

    #188940

    Purpose

    Integration cassette to create N-terminal fusions for fluorescent tagging

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    pBS-SKII-URA-GAL1-HALO-GS
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAG414GAL-ccdB-EGFP

    Plasmid

    #14191

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. E2-Crimson-FKBP(L,V)

    Plasmid

    #85000

    Purpose

    Expresses E2-Crimson-FKBP(L,V) in yeast

    Depositor

    Benjamin Glick

    Article

    Barrero et al Cell Logist. 2016 Jun 24;6(3):e1204848.

    Insert

    E2-Crimson-FKBP(LV)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Dec. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS426-ACT_stable

    Plasmid

    #127609

    Purpose

    high-copy URA-selectable plasmid, Mut ACT1 intron (stable) inserted into URA3

    Depositor

    David Bartel

    Article

    Morgan et al Nature. 2019 Jan;565(7741):606-611. doi:

    Insert

    Stable ACT1 intron (Mut)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPICZc-Ub-R-HsACTB*-thymosinB-8His

    Plasmid

    #111149

    Purpose

    Expresses arginylated human beta-actin fused with thymosin beta and a His tag.

    Depositor

    Mohan Balasubramanian

    Article

    Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

    Insert

    ACTB (ACTB Human)
    

    Use

    Pichia pastoris integration plasmid

    Tags

    Thymosin beta and a His-tag

    Expression

    Yeast

    Mutation

    N-terminal arginylation

    Promoter

    AOX1

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJZC523

    Plasmid

    #62320

    Purpose

    sgRNA for yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pVG45

    Plasmid

    #164703

    Purpose

    yEVenus under the control of CUP1 promoter

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

    Insert

    CUP1pr - yEVenus - CLN2 PEST - ADH1t
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pXP414

    Plasmid

    #26841

    Depositor

    Nancy DaSilva, Suzanne Sandmeyer

    Article

    Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge