8,060 results

1. pJZC595

   Plasmid

   #62322

   Purpose

   Expresses MCP-VP64 and dCas9 in Yeast cells

   Depositor

   Wendell Lim, Stanley Qi

   Article

   Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

   Inserts

   MCP-VP64
   dCas9
   

   Use

   Tags

   VP64

   Expression

   Yeast

   Mutation

   Has nuclease-inactivating mutations in the RuvC1 …

   Promoter

   Tdh3 and pAdh

   Available since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAV0770

   Plasmid

   #133518

   Purpose

   Expression of mTagBFP2 tagged beta-tubulin to monitor microtubules

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pAde6(PmeI)-p(atb2)-mTagBFP2-atb2-terminator(atb2)-hphMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIAeBlue

   Plasmid

   #185891

   Purpose

   In vivo gene amplification (HamAmp) of AeBlue expression module at RPL25 locus

   Depositor

   Claudia Vickers

   Insert

   PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>AeBlue>TPGK1- PBTS1> RPL25(partial; Arm2)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHES834

   Plasmid

   #87947

   Purpose

   Expresses mKate2 under control of GAL1 promoter.

   Depositor

   Hana El-Samad

   Article

   Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

   Insert

   pGAL1-mKate2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pGal1

   Available since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG305GAL-EGFP-ccdB

   Plasmid

   #14305

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGM-ENO2-mCherry-YFP

   Plasmid

   #180570

   Purpose

   Dual fluorescent reporter for 5' UTR activity in yeast mCherry/YFP

   Depositor

   Joel McManus

   Article

   May et al Elife. 2023 May 25;12:e69611. doi: 10.75

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAH6

   Plasmid

   #41830

   Purpose

   Used as a PCR template for tagging yeast proteins with the SNAP tag by homologous recombination and selection for ClonNAT resistance

   Depositor

   Melissa Moore

   Article

   Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

   Type

   Empty backbone

   Use

   Pcr template

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   T7,TEF

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGAD424-hSyntenin2b

   Plasmid

   #89427

   Purpose

   Two-hybrid system

   Depositor

   Fritz Rathjen

   Article

   Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

   Insert

   SDCBP2 (SDCBP2 Human)
   

   Use

   Tags

   Gal4 AD

   Expression

   Yeast

   Mutation

   *see below

   Promoter

   ADH1

   Available since

   July 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS416-PHO5-GFP-Osh3

   Plasmid

   #58839

   Purpose

   Expresses GFP-Tagged Osh3 in yeast

   Depositor

   Tim Levine, Sean Munro

   Article

   Levine et al Mol Biol Cell. 2001 Jun;12(6):1633-44.

   Insert

   Osh3p (OSH3 Budding Yeast)
   

   Use

   Tags

   GFP and Myc

   Expression

   Yeast

   Mutation

   Promoter

   PHO5

   Available since

   Jan. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pRS415-Cas9-VPR

    Plasmid

    #163971

    Purpose

    Trifunctional Cas9-VPR fusion protein plasmid for simultaneous transcriptional activation, transcriptional repression, and genome editing in yeast

    Depositor

    Jiazhang Lian

    Article

    Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

    Insert

    Cas9-VPR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFA6a-KanMX6-Purg1-3FLAG

    Plasmid

    #19354

    Depositor

    Eishi Noguchi

    Article

    Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

    Insert

    urg1 promoter
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. p41Nat MagicFluor

    Plasmid

    #58563

    Purpose

    p41Nat carrying both mating type markers buffered by the Cyc1 terminator

    Depositor

    Leonid Kruglyak

    Insert

    Ste2p-Citrine, Cyc1 term, Ste3p-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAV0612

    Plasmid

    #133505

    Purpose

    Expression of 3xmTagBFP2 targeted to the plasma membrane with an amphipathic helix of RitC

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pAde6(PmeI)-p(tdh1)-3mTagBFP2-RitC-terminator(ScADH1)-bsdMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYEXC3GH

    Plasmid

    #49027

    Purpose

    FX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tag

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Tags

    3C protease site (PreScission site), GFP, and dec…

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG413GPD-ccdB-ECFP

    Plasmid

    #14166

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    ECFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. p416PGK1-OsCYP711A2(pYL770)

    Plasmid

    #178301

    Purpose

    Expresses OsCYP711A2 from O. sativa in Saccharomyces cerevisiae. Centromeric URA, PPGK1-OsCYP711A2-Tpho5

    Depositor

    Yanran Li

    Article

    Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

    Insert

    OsCYP711A2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGK1

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. LDB107

    Plasmid

    #185423

    Purpose

    NUP1-HA CEN TRP1 AmpR. Yeast shuttle plasmid for expression of hemagluttinin tagged NUP1 nucleoporin

    Depositor

    Laura Davis, Ken Belanger

    Article

    Belanger et al J Cell Biol. 1994 Aug;126(3):619-30. doi

    Insert

    NUP1
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPSG830

    Plasmid

    #158601

    Purpose

    tNCS-ePTS1 (2micron)

    Depositor

    John Dueber

    Article

    Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

    Insert

    pTDH3-tNCS-ePTS1
    

    Use

    Tags

    ePTS1

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCfB2804

    Plasmid

    #63644

    Purpose

    pCfB2804 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.

    Depositor

    Jerome Maury, Irina Borodina

    Article

    Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available since

    Aug. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. YIplac211-VPS8-iYFPx6

    Plasmid

    #105272

    Purpose

    Plasmid for pop-in/pop-out replacement of Vps8 with Vps8-iYFPx6.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    Vps8 portion (VPS8 Budding Yeast)
    

    Use

    Tags

    iYFPx6

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge