8,043 results

1. pPOM017_P2_pTIF51

   Plasmid

   #216442

   Purpose

   Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   Promoter tif51
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Promoter

   Available since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPOM016_P2_pRPL2501

   Plasmid

   #216441

   Purpose

   Constitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   Promoter rpl2501
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Promoter

   Available since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDB4878

   Plasmid

   #215498

   Purpose

   A pAde6 backbone integrating plasmid expressing mECitrine under the inducible promoter 41nmt1

   Depositor

   Li-Lin Du

   Article

   Pan et al Methods Mol Biol. 2025;2862:93-102. doi:

   Insert

   mECitrine
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   P41nmt1

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDB5226

   Plasmid

   #215493

   Purpose

   A pAde6 backbone integrating plasmid expressing Pil1-mCherry under the inducible promoter 41nmt1

   Depositor

   Li-Lin Du

   Article

   Pan et al Methods Mol Biol. 2025;2862:93-102. doi:

   Insert

   Pil1-mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   P41nmt1

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDB5225

   Plasmid

   #215492

   Purpose

   A pUra4 backbone integrating plasmid expressing Pil1-mCherry under the inducible promoter 41nmt1

   Depositor

   Li-Lin Du

   Article

   Pan et al Methods Mol Biol. 2025;2862:93-102. doi:

   Insert

   Pil1-mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   P41nmt1

   Available since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDB5227

   Plasmid

   #215494

   Purpose

   A pLys3 backbone integrating plasmid expressing Pil1-mCherry under the inducible promoter 41nmt1

   Depositor

   Li-Lin Du

   Article

   Pan et al Methods Mol Biol. 2025;2862:93-102. doi:

   Insert

   Pil1-mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   P41nmt1

   Available since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDB5228

   Plasmid

   #215495

   Purpose

   A pHis5 backbone integrating plasmid expressing Pil1-mCherry under the inducible promoter 41nmt1

   Depositor

   Li-Lin Du

   Article

   Pan et al Methods Mol Biol. 2025;2862:93-102. doi:

   Insert

   Pil1-mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   P41nmt1

   Available since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDB4875

   Plasmid

   #215497

   Purpose

   A pUra4 backbone integrating plasmid expressing mECitrine under the inducible promoter 41nmt1

   Depositor

   Li-Lin Du

   Article

   Pan et al Methods Mol Biol. 2025;2862:93-102. doi:

   Insert

   mECitrine
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   P41nmt1

   Available since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDB4881

   Plasmid

   #215499

   Purpose

   A pLys3 backbone integrating plasmid expressing mECitrine under the inducible promoter 41nmt1

   Depositor

   Li-Lin Du

   Article

   Pan et al Methods Mol Biol. 2025;2862:93-102. doi:

   Insert

   mECitrine
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   P41nmt1

   Available since

   March 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDB4884

    Plasmid

    #215500

    Purpose

    A pHis5 backbone integrating plasmid expressing mECitrine under the inducible promoter 41nmt1

    Depositor

    Li-Lin Du

    Article

    Pan et al Methods Mol Biol. 2025;2862:93-102. doi:

    Insert

    mECitrine
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    P41nmt1

    Available since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDB5253

    Plasmid

    #215501

    Purpose

    A pUra4 backbone integrating plasmid expressing mECitrine-CtNbr1 under the inducible promoter 41nmt1

    Depositor

    Li-Lin Du

    Article

    Pan et al Methods Mol Biol. 2025;2862:93-102. doi:

    Insert

    mECitrine-CtNbr1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    P41nmt1

    Available since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDB4887

    Plasmid

    #215502

    Purpose

    A pUra4 backbone integrating plasmid expressing mTurquoise2 under the inducible promoter 41nmt1

    Depositor

    Li-Lin Du

    Article

    Pan et al Methods Mol Biol. 2025;2862:93-102. doi:

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    P41nmt1

    Available since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDB4890

    Plasmid

    #215503

    Purpose

    A pAde6 backbone integrating plasmid expressing mTurquoise2 under the inducible promoter 41nmt1

    Depositor

    Li-Lin Du

    Article

    Pan et al Methods Mol Biol. 2025;2862:93-102. doi:

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    P41nmt1

    Available since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDB4893

    Plasmid

    #215504

    Purpose

    A pLys3 backbone integrating plasmid expressing mTurquoise2 under the inducible promoter 41nmt1

    Depositor

    Li-Lin Du

    Article

    Pan et al Methods Mol Biol. 2025;2862:93-102. doi:

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    P41nmt1

    Available since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDB4896

    Plasmid

    #215505

    Purpose

    A pHis5 backbone integrating plasmid expressing mTurquoise2 under the inducible promoter 41nmt1

    Depositor

    Li-Lin Du

    Article

    Pan et al Methods Mol Biol. 2025;2862:93-102. doi:

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    P41nmt1

    Available since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. IL1_1

    Plasmid

    #207623

    Purpose

    Low copy plasmid expressing mTurquoise2 under the TDH3p promoter

    Depositor

    Yvonne Nygård

    Article

    Mormino et al Microb Cell Fact. 2022 Oct 15;21(1):214.

    Insert

    mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYSD101

    Plasmid

    #212925

    Purpose

    Encodes the S. cerevisiae SUC2 (Invertase) protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    SUC2 (SUC2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. P1K-HFL1-8flag-LEU

    Plasmid

    #207035

    Purpose

    Expression of Hfl1-8Flag. Uses auxotrophic marker LEU2(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    HFL1
    

    Use

    Tags

    8xFLAG

    Expression

    Yeast

    Mutation

    Promoter

    pHFL1

    Available since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. ClhN-P1K-3VSV-Atg8(P85L P86L)-MET15

    Plasmid

    #207050

    Purpose

    Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Use

    Tags

    3xVSV

    Expression

    Yeast

    Mutation

    P85L P86L

    Promoter

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ClhN-P1K-3VSV-Atg8(T56A R65A T87A)-MET15

    Plasmid

    #207051

    Purpose

    Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Use

    Tags

    3xVSV

    Expression

    Yeast

    Mutation

    T56A R65A T87A

    Promoter

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. ClhN-P1K-3VSV-Atg8(R65A P85A P86A)-MET15

    Plasmid

    #207052

    Purpose

    Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Use

    Tags

    3xVSV

    Expression

    Yeast

    Mutation

    R65A P85A P86A

    Promoter

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. ClhN-P1K-3VSV-Atg8(R65A P85L P86L)-MET15

    Plasmid

    #207053

    Purpose

    Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Use

    Tags

    3xVSV

    Expression

    Yeast

    Mutation

    R65A P85L P86L

    Promoter

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. ClhN-P1K-3VSV-Atg8(L55W T56A V61W R65A)-MET15

    Plasmid

    #207054

    Purpose

    Expression of 3VSV-Atg8 point mutant. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Use

    Tags

    3xVSV

    Expression

    Yeast

    Mutation

    L55W T56A V61W R65A

    Promoter

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. ClhN-P1K-3VSV-Atg8-MET15

    Plasmid

    #207055

    Purpose

    Expression of 3VSV-Atg8. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).

    Depositor

    Zhiping Xie

    Article

    He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

    Insert

    ATG8
    

    Use

    Tags

    3xVSV

    Expression

    Yeast

    Mutation

    Promoter

    pATG8

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. ClhN-p1k-Cox4-V5-APEX2-Ura

    Plasmid

    #207061

    Purpose

    Expressing Cox4-V5-APEX2. Marker for mitochondrial inner membrane. Tends to generate facture in thin resin sections.

    Depositor

    Zhiping Xie

    Article

    Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

    Insert

    Cox4
    

    Use

    Tags

    V5-APEX2

    Expression

    Yeast

    Mutation

    Promoter

    pCOX4

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. ClhN-p1k-Cox4-GFP-APEX2-Ura

    Plasmid

    #207062

    Purpose

    Expressing Cox4-GFP-APEX2. GFP interferes with APEX staining.

    Depositor

    Zhiping Xie

    Article

    Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

    Insert

    Cox4
    

    Use

    Tags

    GFP-APEX2

    Expression

    Yeast

    Mutation

    Promoter

    pCOX4

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. ClhN-pADH3-Snf7-4V5-APEX2-URA

    Plasmid

    #207065

    Purpose

    Moderate overexpression of Snf7-4V5-APEX2 under ADH3 promoter. Marker for late endosomes. Only partially functional.

    Depositor

    Zhiping Xie

    Article

    Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

    Insert

    Snf7
    

    Use

    Tags

    4xV5-APEX2

    Expression

    Yeast

    Mutation

    Promoter

    pADH3

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. ClhN-pERG6-Pex3-4V5-APEX2-URA

    Plasmid

    #207068

    Purpose

    Moderate overexpression of Pex3-4V5-APEX2 under ERG6 promoter. Marker for peroxisomes.

    Depositor

    Zhiping Xie

    Article

    Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

    Insert

    Pex3
    

    Use

    Tags

    4xV5-APEX2

    Expression

    Yeast

    Mutation

    Promoter

    pERG6

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. ClhN-pLSP1-Pex3-4V5-APEX2-URA

    Plasmid

    #207069

    Purpose

    Moderate overexpression of Pex3-4V5-APEX2 under LSP1 promoter. Marker for peroxisomes.

    Depositor

    Zhiping Xie

    Article

    Li et al mSphere. 2022 Aug 31;7(4):e0010722. doi:

    Insert

    Pex3
    

    Use

    Tags

    4xV5-APEX2

    Expression

    Yeast

    Mutation

    Promoter

    pLSP1

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pPAP_scFv

    Plasmid

    #183733

    Purpose

    Expresses anti-lysozyme scFv antibody

    Depositor

    Jun Ishii

    Article

    Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

    Insert

    Anti-lysozyme scFv
    

    Use

    Tags

    His6-tag

    Expression

    Yeast

    Mutation

    Promoter

    AOX1 promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pPAP_scFv_V50A

    Plasmid

    #183734

    Purpose

    Expresses anti-lysozyme scFv antibody with V50A mutation on MFalpha signal

    Depositor

    Jun Ishii

    Article

    Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

    Insert

    Anti-lysozyme scFv
    

    Use

    Tags

    His6-tag

    Expression

    Yeast

    Mutation

    V50A mutation in MFalpha signal

    Promoter

    AOX1 promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCfB6637

    Plasmid

    #212696

    Purpose

    gRNA targeting to the intergradtion site E3

    Depositor

    Irina Borodina

    Article

    Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

    Insert

    gRNA targeting to the intergradtion site E3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCfB13089

    Plasmid

    #212676

    Purpose

    TEF promoter

    Depositor

    Irina Borodina

    Article

    Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

    Insert

    TEF
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCfB12706

    Plasmid

    #212677

    Purpose

    EXP promoter

    Depositor

    Irina Borodina

    Article

    Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

    Insert

    EXP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCfB12708

    Plasmid

    #212678

    Purpose

    GPD promoter

    Depositor

    Irina Borodina

    Article

    Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

    Insert

    GPD
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCfB13090

    Plasmid

    #212679

    Purpose

    Lip2 terminator

    Depositor

    Irina Borodina

    Article

    Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

    Insert

    Lip2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCfB12710

    Plasmid

    #212680

    Purpose

    Syn8 terminator

    Depositor

    Irina Borodina

    Article

    Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

    Insert

    Syn8
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. PCfB12709

    Plasmid

    #212681

    Purpose

    SynGuo terminator

    Depositor

    Irina Borodina

    Article

    Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

    Insert

    SynGuo
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCfB8860

    Plasmid

    #212697

    Purpose

    gRNA targeting to the intergradtion site E3

    Depositor

    Irina Borodina

    Article

    Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

    Insert

    gRNA targeting to the intergradtion site E3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCfB8856

    Plasmid

    #212701

    Purpose

    gRNA targeting to the intergradtion site C3

    Depositor

    Irina Borodina

    Article

    Jiang et al Metab Eng. 2024 Sep 9:S1096-7176(24)0011

    Insert

    gRNA targeting to the intergradtion site C3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge