8,086 results

1. UG75-ADH1-DGK1-3HA

   Plasmid

   #198595

   Purpose

   DGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6.

   Depositor

   Zhiping Xie

   Article

   Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

   Insert

   DGK1 (DGK1 Budding Yeast)
   

   Use

   Tags

   3HA

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. UG75-ADH1-PAH1-3HA

   Plasmid

   #198597

   Purpose

   PAH1 overexpression. Promotes the formation of diacylglycerol from phosphatidate. Uses antibiotic resistance marker hphMX6.

   Depositor

   Zhiping Xie

   Article

   Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

   Insert

   PAH1 (PAH1 Budding Yeast)
   

   Use

   Tags

   3HA

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAG413GAL-EGFP-ccdB

   Plasmid

   #14309

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCfB6682

   Plasmid

   #106152

   Purpose

   EasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6627, integration into Yarrowia lipolytica chromosomal location IntC_2, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pYIAL31

   Plasmid

   #60807

   Purpose

   used to make mutations in yeast POL1

   Depositor

   Youri Pavlov, Thomas Kunkel

   Article

   Pavlov et al Genetics. 2001 Sep;159(1):47-64.

   Insert

   Pol1 (POL1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   N-terminal part only

   Promoter

   Available Since

   Feb. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-link-yoPSmOrange-SpHis5

   Plasmid

   #44856

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized photoconvertible mOrange with SpHis5 selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoPSmOrange
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 16, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAG416GAL-ccdB-EYFP

   Plasmid

   #14219

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EYFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. ABF2-2EGFP-ter-URA3

   Plasmid

   #184768

   Purpose

   Marker for yeast mitochondria. Integration of 2xGFP tag at ABF2 C terminus. Uses auxotrophic marker URA3(Candida albicans).

   Depositor

   Zhiping Xie

   Article

   Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

   Insert

   ABF2 (ABF2 Budding Yeast)
   

   Use

   Tags

   2EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. KAP95-FLU (113)

   Plasmid

   #24045

   Depositor

   Michael Rout

   Insert

   KAP95 (KAP95 Budding Yeast)
   

   Use

   Tags

   FLU

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 29, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pCfB4796

    Plasmid

    #106131

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntD_1, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CUP Pan1 YFP p316

    Plasmid

    #15592

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    PAN1 (PAN1 Budding Yeast)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pPICZ-mouseGIRK2R201A-eGFP-StII-6xHis

    Plasmid

    #124278

    Purpose

    Expresses mouse GIRK2 R201A as a TEV protease cleavable C-terminal fusion to eGFP, strep tag II, and 6x His tag

    Depositor

    Arthur Laganowsky

    Article

    Liu et al Nat Commun. 2019 Mar 22;10(1):1352. doi:

    Insert

    GIRK2 (Kcnj6 Mouse)
    

    Use

    Tags

    eGFP-StreptagII-6xHis

    Expression

    Yeast

    Mutation

    R201A

    Promoter

    AOX1

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFA6a-kanMX6-P3nmt1-3FLAG

    Plasmid

    #19336

    Depositor

    Eishi Noguchi

    Article

    Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

    Insert

    3FLAG
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. YIplac211-VPS8-iGFPx6

    Plasmid

    #105271

    Purpose

    Plasmid for pop-in/pop-out replacement of Vps8 with Vps8-iGFPx6.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    Vps8 portion (VPS8 Budding Yeast)
    

    Use

    Tags

    iGFPx6

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLK88 - Edit-directing plasmid base

    Plasmid

    #98814

    Purpose

    Edit-directing plasmid base

    Depositor

    Leonid Kruglyak

    Insert

    CAN1.y gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    July 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFTK051

    Plasmid

    #171323

    Purpose

    Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

    Depositor

    Arnold Driessen

    Article

    Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

    Insert

    eBFP fluorescent reporter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    n/a

    Promoter

    Available Since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPT-PCR C2

    Plasmid

    #141247

    Purpose

    PCR cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 2

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Algal Research, 2020. 50, 102014

    Insert

    Mitochondrial Genome of Phaeodactylum tricornutum
    

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Repeat region removed, additional minor mutations…

    Promoter

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pI2-MTU-DO-HIS

    Plasmid

    #160016

    Purpose

    Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). Histidine selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCfB1975 (pUC19-PTEF1-PPGK1)

    Plasmid

    #73296

    Purpose

    Example plasmid containing a bidirectional promoter

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    pTEF1-pPGK1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p405GALS

    Plasmid

    #15965

    Depositor

    Nicolas Buchler, Fred Cross

    Insert

    p405GALS (GAL1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    GALS promoter is between SacI and XbaI restrictio…

    Promoter

    Available Since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge