8,060 results

1. YIplac211-VPS8-iGFPx6

   Plasmid

   #105271

   Purpose

   Plasmid for pop-in/pop-out replacement of Vps8 with Vps8-iGFPx6.

   Depositor

   Benjamin Glick

   Article

   Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

   Insert

   Vps8 portion (VPS8 Budding Yeast)
   

   Use

   Tags

   iGFPx6

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLK88 - Edit-directing plasmid base

   Plasmid

   #98814

   Purpose

   Edit-directing plasmid base

   Depositor

   Leonid Kruglyak

   Insert

   CAN1.y gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRS314-prp16-302

   Plasmid

   #89974

   Purpose

   prp16-302 allele in TRP plasmid

   Depositor

   Christine Guthrie

   Article

   Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

   Insert

   PRP16 (PRP16 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   prp16-302 allele (R456K and G691R)

   Promoter

   Available since

   Aug. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. RB793

   Plasmid

   #199121

   Purpose

   This plasmid contains a barcoding cassette which gets integrated at Neut5 locus in Candida albicans genome

   Depositor

   Richard Bennett

   Insert

   100 bp barcoding cassette
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. RB794

   Plasmid

   #199122

   Purpose

   This plasmid contains a barcoding cassette which gets integrated at Neut5 locus in Candida albicans genome

   Depositor

   Richard Bennett

   Insert

   100 bp barcoding cassette
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRS303 prp8-101

   Plasmid

   #111307

   Purpose

   prp8-101

   Depositor

   Christine Guthrie

   Article

   Umen et al Genetics. 1996 Jun;143(2):723-39.

   Insert

   PRP8 (PRP8 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   prp8-101 (E1960K)

   Promoter

   Available since

   July 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTCW002

   Plasmid

   #83522

   Purpose

   pheromone mediated destablised-GFP expression in yeast

   Depositor

   Claudia Vickers

   Article

   Williams et al ACS Synth Biol. 2013 Mar 15;2(3):136-49.

   Insert

   pFUS1-yEGFPCLN2PEST
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pFUS1

   Available since

   Dec. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMS22H

   Plasmid

   #133904

   Purpose

   Expression of MS2BS-RNAsite hybrid. Homology regions for recombination with pAWH

   Depositor

   Sebastian Maurer

   Article

   Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. p405GALS

   Plasmid

   #15965

   Depositor

   Nicolas Buchler, Fred Cross

   Insert

   p405GALS (GAL1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   GALS promoter is between SacI and XbaI restrictio…

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCfB1975 (pUC19-PTEF1-PPGK1)

    Plasmid

    #73296

    Purpose

    Example plasmid containing a bidirectional promoter

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    pTEF1-pPGK1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNC824

    Plasmid

    #20384

    Depositor

    Beverly Errede

    Article

    Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

    Insert

    UAS cassette (FUS1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    X=UbiY (see schematic)

    Promoter

    Available since

    March 6, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFA6a-kanMX6-P3nmt1-3FLAG

    Plasmid

    #19336

    Depositor

    Eishi Noguchi

    Article

    Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

    Insert

    3FLAG
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. LD220

    Plasmid

    #69188

    Purpose

    Integration of 2GFP tag at Atg20 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg20-2EGFP
    

    Use

    Tags

    2xEGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHM4 (U6 in pSE360)

    Plasmid

    #111281

    Purpose

    U6 snRNA coding sequence in pSE360(Ura) plasmid

    Depositor

    Christine Guthrie

    Article

    Madhani et al Genes Dev. 1990 Dec;4(12B):2264-77.

    Insert

    SNR6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJK148-Pnmt41-GBP-mCherry(N)-leu1+

    Plasmid

    #89070

    Purpose

    genomic GBP-mCherry epitope N-terminal tagging at leu1+ locus in S. pombe

    Depositor

    Quanwen Jin

    Article

    Chen et al J Cell Sci. 2017 Jan 12. pii: jcs.198457

    Type

    Empty backbone

    Use

    Genomic integration in s. pombe

    Tags

    GBP-mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTH644-CENBEVY

    Plasmid

    #29695

    Depositor

    Tobias von der Haar

    Article

    Chu et al Nucleic Acids Res. 2011 Aug;39(15):6705-

    Insert

    bidirectional promoter cassette from pBEVY-U
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. LHP336 - HA-Ub-K48R

    Plasmid

    #32164

    Depositor

    Linda Hicke

    Insert

    synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    K48R

    Promoter

    CUP1

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

18. LHP416-PAN1-HA

    Plasmid

    #32159

    Depositor

    Linda Hicke

    Insert

    PAN1 (PAN1 Budding Yeast)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pGBW-m4046381

    Plasmid

    #149010

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. YIplac211-msGFP2-STE13

    Plasmid

    #177106

    Purpose

    Expresses msGFP2-Ste13 in yeast cells

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

    Insert

    STE13 (STE13 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge