8,432 results

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pgRNA-backbone

Plasmid

#172517

Purpose

Yeast plasmid encoding a 2-kb gRNA-placeholder flanked by BsmBI/Esp3I restriction sites to facilitate gRNA insertion

Depositor

Leonid Kruglyak

Publication

Schubert et al Elife. 2022 Nov 3;11. pii: 79525. doi: 1

Insert

gRNA cassette

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-URA-GAL1-fSNAP-3xHA

Plasmid

#188935

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Publication

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

URA-GAL1-fSNAP-3xHA

Expression

Yeast

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-HALO-Hygromycin

Plasmid

#188929

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Publication

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-HALO-Hygromycin

Expression

Yeast

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fCLIP-URA

Plasmid

#188922

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Publication

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fCLIP-URA

Expression

Yeast

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fCLIP-NAT

Plasmid

#188921

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Publication

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fCLIP-NAT

Expression

Yeast

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fSNAP-Kan

Plasmid

#188918

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Publication

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fSNAP-Kan

Expression

Yeast

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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p416PGK1-OsCYP711A2(pYL770)

Plasmid

#178301

Purpose

Expresses OsCYP711A2 from O. sativa in Saccharomyces cerevisiae. Centromeric URA, PPGK1-OsCYP711A2-Tpho5

Depositor

Yanran Li

Publication

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

OsCYP711A2

Expression

Yeast

Promoter

PGK1

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP5EC4

Plasmid

#185880

Purpose

A plasmid for in vivo gene amplification (HapAmp) at SEC23 locus (9 copy)

Depositor

Claudia Vickers

Publication

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Type

Empty backbone

Expression

Yeast

Mutation

None

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pIAeBlue

Plasmid

#185891

Purpose

In vivo gene amplification (HapAmp) of AeBlue expression module at RPL25 locus

Depositor

Claudia Vickers

Publication

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Insert

PRPL25(Arm 1)> Kl.LEU2>TKl.LEU2- TRPL25(Arm 3)- ARS305- PALD6>AeBlue>TPGK1- PBTS1> RPL25(partial; Arm2)

Expression

Yeast

Mutation

None

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pIT6EG7ml

Plasmid

#185886

Purpose

In vivo gene amplification (HapAmp) of limonene synthetic module at RPL25 locus (14 copy).

Depositor

Claudia Vickers

Publication

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Insert

see comments

Expression

Yeast

Mutation

ERG20 F96W N127W

Available since

Sept. 1, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pILGFP5EA4

Plasmid

#185879

Purpose

A plasmid for in vivo gene amplification (HapAmp) at SEC23 locus (4 copy)

Depositor

Claudia Vickers

Publication

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Type

Empty backbone

Expression

Yeast

Mutation

None

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP3AG4ARSd

Plasmid

#185876

Purpose

A plasmid without an ARS sequence for in vivo gene amplification (HapAmp) at RPL25 locus (~12 copy)

Depositor

Claudia Vickers

Publication

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Type

Empty backbone

Expression

Yeast

Mutation

None

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pJCHph

Plasmid

#185863

Purpose

The CRISPR/Cas9-and-gRNA-expressing plasmid with a hygromycin B-resistent selectable marker

Depositor

Claudia Vickers

Publication

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Insert

PTDH3-Cas9(N); PSNR52-srRNA-TSUP4; HphMX6

Expression

Yeast

Mutation

None

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pIRTetR-GAL80

Plasmid

#185853

Purpose

Plugging the mechanism of tetracycline-mediated repression of GAL promoters in Saccharomyces cerevisiae

Depositor

Claudia Vickers

Publication

Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

Insert

gal80Arm1-PAgTEF1-KanMX4-TAgTEF1-PHAC1>TetR>TUP1>PABF1- PTEF1+[TetO]>GAL80Arm2

Expression

Yeast

Mutation

None

Available since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pINER5

Plasmid

#185895

Purpose

In vivo gene amplification (HapAmp) of nerolidol synthetic module at RPL25 locus (15 copy).

Depositor

Claudia Vickers

Publication

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Insert

PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PSk.GAL2>YFAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2

Expression

Yeast

Mutation

None

Available since

Aug. 25, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pJRoC30_VP5H

Plasmid

#188061

Purpose

PROSS-designed versatile peroxidase 5H

Depositor

Sarel Fleishman

Publication

Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-35

Insert

PROSS-designed versatile peroxidase from Pleurotus ostreatus

Tags

S. cerevisiae native α-factor prepro-leader signa…

Expression

Yeast

Mutation

43 PROSS mutations, described in publication

Available since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9336 (pgRNA_II-1_NatMX)

Plasmid

#161589

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site II-1

Depositor

Irina Borodina

Publication

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK28 pHomL-TDH3p-GFPdropout-Link-Neon(gfp)-SSA1t-HomR (AmpR)

Plasmid

#179012

Purpose

Parent vector for markerless yeast integration cassettes with C-terminal Neon(gfp)-tagged genes

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Type

Empty backbone

Expression

Yeast

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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KBB364

Plasmid

#185083

Purpose

RFA1 fused to GFP under control of GAL1 promoter

Depositor

Ken Belanger

Publication

Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

Insert

RFA1

Tags

GFP

Expression

Yeast

Promoter

GAL1

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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p416-GPD-DIPP1-HA

Plasmid

#183946

Purpose

Expresses human HA-tagged DIPP1 from yeast GPD promoter

Depositor

Michael Downey

Publication

McCarthy et al mBio. 2022 Aug 30;13(4):e0039022. doi: 1

Insert

DIPP1 (NUDT3 Human)

Tags

HA

Expression

Yeast

Mutation

WT

Promoter

GPD

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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