7,935 results

1. 426Gal-FUS

   Plasmid

   #29591

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 1, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pICXNHG3

   Plasmid

   #49021

   Purpose

   FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site

   Depositor

   Eric Geertsma, Raimund Dutzler

   Article

   Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

   Type

   Empty backbone

   Use

   Tags

   3C protease site (PreScission site), GFP, and dec…

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available since

   Jan. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDRF1 ymStayGold-T2A-mCherry

   Plasmid

   #219843

   Purpose

   equimolar expression of ymStayGold and mCherry in budding yeast

   Depositor

   Bas Teusink

   Insert

   ymStayGold-T2A-mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA6a-ymTurquoise2-CaURA3

   Plasmid

   #118458

   Purpose

   Yeast tagging vector to create a gene fusion with yeast optimized mTq2 with CaUra3 selection

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymTurquoise2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPS1622

   Plasmid

   #8854

   Depositor

   Pamela Silver

   Article

   Prinz et al J Cell Biol. 2000 Aug 7. 150(3):461-74.

   Insert

   Sec63-sGFP (SEC63 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   sGFP=S65T V163A mutant of GFP

   Promoter

   Available since

   Aug. 3, 2005

   Availability

   Academic Institutions and Nonprofits only

6. pWS176

   Plasmid

   #90963

   Purpose

   Cas9 gap repair vector - ZeoR

   Depositor

   Tom Ellis

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBS-SKII-3XHA-HALO-Kanamycin

   Plasmid

   #188933

   Purpose

   Integration cassette to create C-terminal fusions for fluorescent tagging

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   3XHA-HALO-Kanamycin
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. HC078 (3xHA-Atg13)

   Plasmid

   #59544

   Purpose

   Expresses yeast Atg13 under the endogenous promoter with a 3xHA N-terminal tag

   Depositor

   Daniel Klionsky

   Article

   Cheong et al Mol Biol Cell. 2008 Feb;19(2):668-81. Ep

   Insert

   ATG13 (ATG13 Budding Yeast)
   

   Use

   Tags

   3xHA

   Expression

   Bacterial and Yeast

   Mutation

   AvrII site created after start codon

   Promoter

   ATG13

   Available since

   Oct. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

9. BB3rN_14*

   Plasmid

   #98550

   Purpose

   empty BB3 for insertion and overexpression of 1 gene in P. pastoris

   Depositor

   Michael Sauer, Diethard Mattanovich, Brigitte Gasser

   Article

   Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Feb. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPICZc-HsACTB*-thymosinB-8His

    Plasmid

    #111146

    Purpose

    Expresses human beta-actin fused with thymosin beta and a His tag.

    Depositor

    Mohan Balasubramanian

    Article

    Hatano et al J Cell Sci. 2018 Mar 13. pii: jcs.213827

    Insert

    ACTB (ACTB Human)
    

    Use

    Pichia pastoris integration

    Tags

    Thymosin beta and a His-tag

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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