8,388 results
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Plasmid#67549PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked bleMX marker, integration into S. cerevisiae XI-5 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only
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pCfB2400
Plasmid#67552PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked dsdA marker, integration into S. cerevisiae XII-1 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2198
Plasmid#67554PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae XII-4 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2228
Plasmid#67540PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-4 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS426-GFP-Osh2-PH
Plasmid#71373PurposeFor analysis of PH domain localization in yeastDepositorInsertOsh2-PH
TagsEGFPExpressionYeastMutationPleckstrin homology (PH) domain; aa 277-398Available SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK370
Plasmid#73595PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-2) with C-terminal c-myc epitopeDepositorInsertsdelta-12 fatty acid acetylenase
delta-12 fatty acid desaturase (form 2)
TagsFLAG tag and c-myc tagExpressionYeastMutationSilent mutation in Arg299 codon.PromoterGAL1 and GAL10Available SinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
LD223
Plasmid#69190PurposeIntegration of 2GFP tag at Atg23 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg23-2EGFP
Tags2xEGFPExpressionYeastAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
LD109
Plasmid#69164PurposeIntegration of GFP tag at Atg9 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg9-EGFP
TagsEGFPExpressionYeastAvailable SinceOct. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
LD118
Plasmid#69170PurposeIntegration of GFP tag at Atg18 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg18-EGFP
TagsEGFPExpressionYeastAvailable SinceSept. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSP303 pRS306 pRPS2-Far1DS-NLS-mCherry
Plasmid#68279PurposeSKARS: Fus3 MAPK activity sensor: Far1DS 2xNLS mCherryDepositorInsertFar1DS NLS mCherry
TagsmCherryExpressionYeastPromoterRPS2Available SinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pED73 pRS304 pRPS2-SteDS-NLS-mCitrine
Plasmid#68278PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCitrineDepositorInsertSte7DS NLS mCitrine
TagsmCitrineExpressionYeastPromoterpRPS2Available SinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRG236
Plasmid#64534PurposeIntegrative E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRG221
Plasmid#64520PurposeEpisomal E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP39
Plasmid#65517PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-edAFPt9-attR1 and attR2-t7edCFPt9-cMycExpressionYeastPromoterpPMA1Available SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP43
Plasmid#65519PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-edAFPt9-attR1 and attR2-edCer-cMycExpressionYeastPromoterpPMA1Available SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRPR1_c4gRNA_RPR1t
Plasmid#64382Purposeencodes c4 gRNADepositorInsertc4 gRNA
ExpressionYeastAvailable SinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJW1517
Plasmid#62387PurposepFA6a Osm1(M33A)-yoEGFP::LEU2DepositorInsertOSM1-EGFP fusion
TagsEGFPExpressionYeastMutationMethionine 33 mutated to AlaninePromoterAdh1pAvailable SinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
6x_a1gRNAOp_pCYC1m_yeBFP2
Plasmid#64392Purposeencodes six a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFPDepositorInsertyeast enhanced BFP2
ExpressionYeastAvailable SinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
12x_a1gRNAOp_pCYC1m_yeBFP2
Plasmid#64393Purposeencodes twelve a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFPDepositorInsertyeast enhanced BFP2
ExpressionYeastPromoterpCYC1Available SinceMay 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRPR1_c8gRNA_RPR1t
Plasmid#64386Purposeencodes c8 gRNADepositorInsertc8 gRNA
ExpressionYeastAvailable SinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only