8,128 results

1. pRS316-RGR-GFP-mHDV

   Plasmid

   #51058

   Purpose

   Same as pRS316-RGR-GFP except for the HDV ribozyme region which has a 15 bp deletion at its 3' end.

   Depositor

   Yunde Zhao

   Article

   Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

   Insert

   RGR-GFP
   

   Use

   CRISPR

   Expression

   Bacterial and Yeast

   Promoter

   Yeast ADH1 promoter

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS316-RGR-GFP-mHH

   Plasmid

   #51057

   Purpose

   Same as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end.

   Depositor

   Yunde Zhao

   Article

   Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

   Insert

   RGR-GFP
   

   Use

   CRISPR

   Expression

   Bacterial and Yeast

   Promoter

   Yeast ADH1 promoter

   Available Since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAG415GPD-Cerulean-ccdB

   Plasmid

   #14410

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   Cerulean

   Expression

   Yeast

   Available Since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDRF’- AmTryoshka1;3-GS

   Plasmid

   #100967

   Purpose

   Yeast expression of control vector for fluorescent sensors reporting the activity of ammonium transceptors.

   Depositor

   Wolf Frommer

   Article

   Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

   Insert

   AmTryoshka1;3-GS (AMT1;3 Mustard Weed)
   

   Expression

   Yeast

   Promoter

   PMA

   Available Since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWS033

   Plasmid

   #123056

   Purpose

   Green fluorescent protein

   Depositor

   Tom Ellis

   Article

   Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

   Insert

   sfGFP [3]
   

   Expression

   Yeast

   Available Since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNIA-CEN-Venus-FLAG-LINXa4-Bre1

   Plasmid

   #73618

   Purpose

   Visualizing the nuclear translocation of LINXa4-Bre1 in yeast.

   Depositor

   Brian Kuhlman

   Article

   Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

   Insert

   Venus
   

   Use

   Synthetic Biology

   Tags

   FLAG

   Expression

   Yeast

   Mutation

   Bre1 point mutations K8A, K9A and K11A

   Promoter

   ADH1

   Available Since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG424GAL-ccdB-EYFP

   Plasmid

   #14223

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EYFP

   Expression

   Yeast

   Available Since

   March 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pFA6a-kanMX6-P41nmt1-mEGFP

   Plasmid

   #105140

   Purpose

   Yeast gene targeting

   Depositor

   Jian-Qiu Wu

   Article

   Laporte et al Mol Biol Cell. 2012 Aug;23(16):3094-110.

   Insert

   kanMX6-P41nmt1-mEGFP
   

   Tags

   41nmt1-mEGFP

   Expression

   Bacterial and Yeast

   Mutation

   A206K

   Promoter

   41nmt1

   Available Since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. LD214

   Plasmid

   #69185

   Purpose

   Integration of 2GFP tag at Atg14 C terminus, use auxotrophic marker URA3(Candida albicans)

   Depositor

   Zhiping Xie

   Article

   Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

   Insert

   Atg14-2EGFP
   

   Tags

   2xEGFP

   Expression

   Yeast

   Available Since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. LD205

    Plasmid

    #69180

    Purpose

    Integration of 2GFP tag at Atg5 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg5-2EGFP
    

    Tags

    2xEGFP

    Expression

    Yeast

    Available Since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJT45_GalL_nCDA1-BE3

    Plasmid

    #145038

    Purpose

    Expresses nCDA1-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    nCDA1-BE3
    

    Use

    CRISPR

    Expression

    Yeast

    Mutation

    spCas9(D10A)

    Promoter

    GalL

    Available Since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJAID5VY

    Plasmid

    #165048

    Purpose

    Integrative expression of CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>CUP1-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    

    Expression

    Yeast

    Mutation

    OsTIR1 is codon-optimised for expression in S. ce…

    Available Since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPIC-mouseKir3.2-mC-S

    Plasmid

    #140745

    Purpose

    Expression of mouse Kir3.2 fused to mCherry and Streptag II

    Depositor

    Arthur Laganowsky

    Article

    Qiao et al Biochemistry. 2020 May 6. doi: 10.1021/a

    Insert

    Kir3.2 (Kcnj6 Mouse)
    

    Tags

    mCherry with Streptag II

    Expression

    Yeast

    Promoter

    AOX1

    Available Since

    May 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAG424GAL-EYFP-ccdB

    Plasmid

    #14343

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Available Since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGBW-m4046380

    Plasmid

    #148977

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pGBW-m4046710

    Plasmid

    #149178

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available Since

    May 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pAG414GAL-ccdB-EYFP

    Plasmid

    #14215

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Available Since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFA6a-hygML

    Plasmid

    #73892

    Purpose

    fission yeast drug selection marker

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Insert

    hygML
    

    Expression

    Bacterial and Yeast

    Available Since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPS2189

    Plasmid

    #1381

    Depositor

    Pamela Silver

    Article

    Hood et al J Cell Sci 2001 Feb;114(Pt 3):589-97.

    Insert

    Gal-Clb2-GFP (CLB2 Budding Yeast)
    

    Tags

    GFP

    Expression

    Yeast

    Available Since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

20. YIp211-P30-His-pol30(K127/164R)-3'

    Plasmid

    #99549

    Purpose

    Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127/164R; URA3 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    POL30 (POL30 Budding Yeast)
    

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    K127R, K164R

    Promoter

    POL30

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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