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pHUK

Plasmid

#64183

Purpose

harbours marker genes HIS3, URA3 and LYS2

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pBSWHhc

Plasmid

#133901

Purpose

Expression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046342

Plasmid

#149077

Purpose

Yeast Expression plasmid for SARS-CoV-2 3'-to-5' exonuclease

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable thrombin;StrepII

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046699

Plasmid

#149153

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp2

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 nsp2 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Yeast

Mutation

wt

Promoter

pGAL1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046393

Plasmid

#149132

Purpose

Yeast Expression plasmid for SARS-CoV-2 endoRNAse

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 endoRNAse (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

wt

Promoter

pGAL1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046470

Plasmid

#149046

Purpose

Yeast Expression plasmid for SARS-CoV-2 M (membrane)

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable thrombin;StrepII

Expression

Yeast

Mutation

wt

Promoter

pGAL1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046467

Plasmid

#149039

Purpose

Yeast Expression plasmid for SARS-CoV-2 M (membrane)

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 M (membrane) (M Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

wt

Promoter

pGAL1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046181

Plasmid

#149090

Purpose

Yeast Expression plasmid for SARS-CoV-2 M (membrane)

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 M (membrane) (M Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable thrombin;StrepII

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available since

May 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBKT7-PRP5

Plasmid

#87592

Purpose

Encodes Y2H Gal4 DNA binding domain for Prp5

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP5 (PRP5 Budding Yeast)

Use

Tags

GAL4 BD and Myc

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046295

Plasmid

#145513

Purpose

Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 N (nucleocapsid) (N Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

wt

Promoter

pGAL1

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pFA6a-kanMX6-P81nmt1-mCFP

Plasmid

#105142

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu, Thomas Pollard

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

kanMX6-P81nmt1-mCFP

Use

Tags

81nmt1-mCFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

81nmt1

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P41nmt1-mCFP

Plasmid

#105139

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu, Thomas Pollard

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

kanMX6-P41nmt1-mCFP

Use

Tags

41nmt1-mCFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

41nmt1

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046707

Plasmid

#149225

Purpose

Yeast Expression plasmid for SARS-CoV-2 ORF8 protein

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 ORF8 protein (ORF8 Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Yeast

Mutation

wt

Promoter

pGAL1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pGBW-m4046626

Plasmid

#149220

Purpose

Yeast Expression plasmid for SARS-CoV-2 M (membrane)

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 M (membrane) (M Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Yeast

Mutation

wt

Promoter

pTEF1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046703

Plasmid

#149215

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp6

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 nsp6 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Yeast

Mutation

wt

Promoter

pTEF1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046667

Plasmid

#149155

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp4

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 nsp4 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Yeast

Mutation

wt

Promoter

pTEF1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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piHGpd/p82

Plasmid

#1105

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

wild-type HSP82 regulated by the strong constitut…

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
416Gal-FUS-R524T-YFP

Plasmid

#29627

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Use

Tags

YFP

Expression

Yeast

Mutation

R524T

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pGBW-m4046679

Plasmid

#149209

Purpose

Yeast Expression plasmid for SARS-CoV-2 3C-like proteinase

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 3C-like proteinase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046700

Plasmid

#149205

Purpose

Yeast Expression plasmid for SARS-CoV-2 ORF8 protein

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 ORF8 protein (ORF8 Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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