8,432 results
-
Plasmid#178991PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = LEU2DepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyExpressionYeastAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK04 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitHygR,AmpR)
Plasmid#178988PurposeSp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyExpressionYeastAvailable sinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK085
Plasmid#171357PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertTtRNA[Leu] P. rubens Pol-III terminator
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK086
Plasmid#171358PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertP-ANtRNA[Arg21]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK053
Plasmid#171325PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertalcR ANIA_08978 transcriptional activator (no intron)
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK047
Plasmid#171319PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertDsRed-NLS fluorescent reporter
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK033
Plasmid#171305PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertCPnirA AN0098 core promoter (for fusion)
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK026
Plasmid#171298PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertCPnirA AN0098 (no UAS) core promoter
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK013
Plasmid#171285PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertPpcbC Pc21g21380 promoter
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK011
Plasmid#171283PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertPpcbAB Pc21g21390 promoter
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFTK009
Plasmid#171281PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertPglaA An03g06550 promoter
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZEN
Plasmid#129113PurposeZ. rouxii episomal vector containing ClonNAT resistanceDepositorTypeEmpty backboneExpressionYeastAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZCAG
Plasmid#129114PurposeZ. rouxii centromeric vector containing G418 resistanceDepositorTypeEmpty backboneUseCre/LoxExpressionYeastAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMLB10
Plasmid#177273PurposeGAL1p:T7RNAP(F11L;T613A):CYC1tDepositorInsertT7RNAP(F11L;T613A)
ExpressionBacterial and YeastMutationF11L T613APromoterGAL1Available sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH-mRuby2
Plasmid#176547PurposeExpression of mRuby2 for auxotrophic selection in the absence of histidineDepositorInsertyomRuby2
ExpressionYeastPromoterTDH1(GAP)Available sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH-eCFP
Plasmid#176555PurposeExpression of eCFP for auxotrophic selection in the absence of histidineDepositorInserteCFP
ExpressionYeastPromoterTDH1(GAP)Available sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pL-eCFP
Plasmid#176556PurposeExpression of eCFP for auxotrophic selection in the absence of leucineDepositorInserteCFP
ExpressionYeastPromoterTDH1(GAP)Available sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU-eCFP
Plasmid#176557PurposeExpression of eCFP for auxotrophic selection in the absence of uracilDepositorInserteCFP
ExpressionYeastPromoterTDH1(GAP)Available sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM-TagRFP657
Plasmid#176563PurposeExpression of TagRFP657 for auxotrophic selection in the absence of methionineDepositorInsertTagRFP657
ExpressionYeastPromoterTDH1(GAP)Available sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV490
Plasmid#177698PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.DepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterRHR2p (Debaryomyces hansenii ) and TDH3p (Candida…Available sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by