8,057 results
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Plasmid#74251PurposeIntermediate cloning vector, with MCS2 containing inducible stable peptide driven by pSTL1 (Venus 2xNLSs SZ1).DepositorInsertInducible peptide of the dPSTR
UseTagsVenusExpressionBacterial and YeastMutationPromoterpSTL1Available sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDA199
Plasmid#74275PurposepSTL1-dPSTRy(4,3)DepositorInsertpSTL1-dPSTRy(4,3)
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNIA-CEN-FLAG-LINXa4-Bre1
Plasmid#73617PurposeControl of the E3 ligase Bre1 with LINXa in yeast.DepositorInsertLINXa4-Bre1dNLS
UseSynthetic BiologyTagsFLAGExpressionYeastMutationBre1 point mutations K8A, K9A and K11APromoterADH1Available sinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK139
Plasmid#73315PurposePas2p peroxisomal membrane biogenesis proteinDepositorInsertPas2p peroxisomal membrane targeting protein
UseTagsHis6 and c-mycExpressionYeastMutationEncodes a glycine-204 to serine mutationPromoterAOX1Available sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pED54pRS306 pRPS2-Ste7DS NLS(4E)-mCherry
Plasmid#68276PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4E) mCherry Non functional sensor with phospho-mimicking NLSDepositorInsertSte7DS NLS(4E) mCherry
UseTagsmCherryExpressionYeastMutationPromoterRPS2Available sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2336
Plasmid#67541PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked SpHIS5 marker, integration into S. cerevisiae XII-5 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK275
Plasmid#73363PurposeEncodes Crepis alpina delta-8 sphingolipid desaturase (SLD1)DepositorInsertdelta-8 sphingolipid desaturase
UseTagsExpressionYeastMutationmissing residues 1-6PromoterGAL1Available sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK138
Plasmid#73314PurposePas2p peroxisomal membrane targeting sequenceDepositorInsertPas2p (peroxisomal membrane targeting region)
UseTagsHis6 and c-mycExpressionYeastMutationencodes residues 1-42PromoterAOX1Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK141
Plasmid#73316PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with Pas2p signal sequence, c-myc tag, and His6-tagDepositorInsertdelta-12 fatty acid acetylenase
UseTagsHis6, Pas2p signal sequence, and c-mycExpressionYeastMutationsynthetic genePromoterAOX1Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK137
Plasmid#73313PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with c-myc tag and His6-tagDepositorInsertdelta-12 fatty acid acetylenase
UseTagsHis6 and c-mycExpressionYeastMutationsynthetic genePromoter5'-AOX1Available sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTD125 GFP:POP1
Plasmid#71387Purposelocalization of yeast RNase POP1 (RNase MRP binding protein) in yeastDepositorInsertPOP1 (POP1 Budding Yeast)
UseTagsGFPExpressionYeastMutationPromoterACT1Available sinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUG35-YDL183c-GFP
Plasmid#70161Purposeyeast expression of YDL183c with GFP fusionDepositorInsertYDL183c
UseTagsyEGFPExpressionYeastMutationPromoterMET17Available sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
YCp33-YDL183c-HA
Plasmid#70164Purposeyeast expression of YDL183c from its own promoter and in fusion with a triple HA epitope at the C-terminal endDepositorInsertYDL183c
UseTags3xHAExpressionYeastMutationPromoterYDL183c (217 nucleotides upstream of the ATG)Available sinceFeb. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK349
Plasmid#71566PurposeProduces Helianthus annuus delta12 fatty acid acetylenase (vFAD2) in yeastDepositorInsertdelta12 fatty acid acetylenase
UseTagsExpressionBacterial and YeastMutationPromoterGAL1Available sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDS2-aurZ
Plasmid#70151PurposeFusarium graminearum AurZ encoded on CEN/ARS yeast shuttle vector with DS2 selection markerDepositorInsertaurZ
UseTagsExpressionYeastMutationPromoterTefAvailable sinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pJK274
Plasmid#71564PurposeProduces Crepis alpina delta12 fatty acid desaturase (FAD2-3) in yeastDepositorInsertdelta12 fatty acid desaturase (form 3)
UseTagsExpressionBacterial and YeastMutationPromoterGAL1 promoterAvailable sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK272
Plasmid#71562PurposeProduces Crepis alpina delta12 fatty acid acetylenase (vFAD2) in yeastDepositorInsertdelta12 fatty acid acetylenase
UseTagsExpressionBacterial and YeastMutationPromoterGAL1Available sinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDS3-aurJ
Plasmid#70152PurposeFusarium graminearum AurJ encoded on CEN/ARS yeast shuttle vector with DS3 selection markerDepositorInsertaurJ
UseTagsExpressionYeastMutationPromoterGPD1Available sinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDS3-X4
Plasmid#70140PurposeYeast chr. integration vector with DS3 selection markerDepositorInsertDS3
UseTagsExpressionYeastMutationPromoterAgTEF1Available sinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS425-M25-Afg1
Plasmid#61720Purposeyeast Afg1 AAA protein in yeast expression vectorDepositorInsertAfg1 (AFG1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS415-Cox15-13xMyc
Plasmid#61721Purposeyeast Cox15 heme A synthase with C-terminal 13xMyc tag in low-copy yeast expression vectorDepositorInsertCox15 (COX15 Budding Yeast)
UseTags13xMycExpressionYeastMutationPromoterAvailable sinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfB2229
Plasmid#67537PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked CaLYS5 marker, integration into S. cerevisiae XI-5 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
LD123
Plasmid#69173PurposeIntegration of GFP tag at Atg23 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg23-EGFP
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
LD114
Plasmid#69167PurposeIntegration of GFP tag at Atg14 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg14-EGFP
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
LD105
Plasmid#69162PurposeIntegration of GFP tag at Atg5 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg5-EGFP
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
BS-Trp1
Plasmid#69196Purposecomplementation of yeast auxotrophic marker TRP1. Although this is the plasmid described in the paper, the depositor notes that it has a low integration efficiency and Plasmid 69503 should be used.DepositorInsertTRP1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCyc-Bem1
Plasmid#66573PurposeExpress an additional copy of Bem1, integrated at TRP locusDepositorInsertBem1
UseTagsExpressionYeastMutationF99I*(see below)PromoterpCyc1Available sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
LD131
Plasmid#69177PurposeIntegration of GFP tag at Atg31 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg31-EGFP
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
LD129
Plasmid#69176PurposeIntegration of GFP tag at Atg29 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg29-EGFP
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
LD124
Plasmid#69174PurposeIntegration of GFP tag at Atg24 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg24-EGFP
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
LD120
Plasmid#69171PurposeIntegration of GFP tag at Atg20 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg20-EGFP
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceSept. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pED55 pRS306 pRPS2-SteND-NLS-mCherry
Plasmid#68277PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7ND 2xNLS mCherry Non functional sensor with non-docking mutationDepositorInsertSte7ND NLS mCherry
UseTagsmCherryExpressionYeastMutationPromoterpRPS2Available sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pED53 pRS306 pRPS2-Ste7DS NLS(4A)-mCherry
Plasmid#68275PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4A) mCherry Non functional sensor with non-phosphorylatable NLSDepositorInsertSte7DS NLS(4A) mCherry
UseTagsmCherryExpressionYeastMutationPromoterpRPS2Available sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCyc-Cdc24
Plasmid#66574PurposeExpress an additional copy of Cdc24, integrated at TRP locusDepositorInsertCdc24
UseTagsExpressionYeastMutationPromoterpCyc1Available sinceJuly 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRPR1_g2gRNA_RPR1t
Plasmid#64388Purposeencodes g2 gRNADepositorInsertg2 gRNA
UseTagsExpressionYeastMutationPromoterpRPR1Available sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB3 Cterminal tail delta K
Plasmid#60404PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta3 tubulin Cterminal tail (TUBB3) without last 4 amino acids--QGPKDepositorInsertTUB2
UseTagsExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable sinceMay 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB1 Cterminal tail
Plasmid#60400PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta6 tubulin Cterminal tail (TUBB1) using a GAL promoterDepositorInsertTUB2
UseTagsExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable sinceMay 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB3 Cterminal tail
Plasmid#60397PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta3 tubulin Cterminal tail (TUBB3) using a GAL promoterDepositorInsertTUB2
UseTagsExpressionYeastMutationC127S; TUB2 amino acids 429 - end, replaced with …PromoterGALAvailable sinceMay 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRPR1_c2gRNA_RPR1t
Plasmid#64380Purposeencodes c2 gRNADepositorInsertc2 gRNA
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only