7,942 results

1. pFA6a-CLIP-KanMX6

   Plasmid

   #87025

   Purpose

   Tag S. pombe genes in C terminal with CLIP

   Depositor

   Julien Berro

   Insert

   CLIP
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMEL12

   Plasmid

   #107918

   Purpose

   hph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

   Depositor

   Jean-Marc Daran

   Article

   Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

   Insert

   gRNA-CAN1.Y
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMZ288

   Plasmid

   #59897

   Purpose

   Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: sgRNA targeted against ade6+

   Depositor

   Mikel Zaratiegui

   Article

   Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

   Inserts

   Cas9
   rrk1:sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Silent muation of the CspCI site

   Promoter

   Available since

   Oct. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAV113

   Plasmid

   #63180

   Purpose

   pRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGAD-Atg17

   Plasmid

   #121386

   Purpose

   For Yeast-two-hybrid analysis

   Depositor

   Daniel Klionsky

   Article

   Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

   Insert

   ATG17
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH1 promoter

   Available since

   March 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. PP324

   Plasmid

   #78982

   Purpose

   246B. Promoter: GAP6, minimal Promoter: AOX1. Figure 5. Expresses rHGH and IFNa2b.

   Depositor

   Timothy Lu

   Article

   Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

   Inserts

   rHGH
   IFNa2b
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOP459

   Plasmid

   #85933

   Purpose

   Encodes the Anti-Ebola 2G4 monoclonal antibody. Chimeric - mouse variable regions, human constant regions.

   Depositor

   Timothy Lu

   Article

   Purcell et al ACS Synth Biol. 2017 Aug 17. doi: 10.102

   Insert

   Anti-Ebola 2G4 monoclonal antibody
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pILGFP5EF3

   Plasmid

   #185881

   Purpose

   A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (13 copy)

   Depositor

   Claudia Vickers

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. ZJOM2

   Plasmid

   #133633

   Purpose

   Integration of VRG4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast early golgi.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-VRG4-GFP (VRG4 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. BS-Leu2

    Plasmid

    #69200

    Purpose

    complementation of yeast auxotrophic marker LEU2

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    LEU2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge