8,388 results
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Plasmid#73366PurposeEncodes Crepis alpina plastid omega-3 fatty acid desaturase (FAD7)DepositorInsertplastid omega-3 fatty acid desaturase
ExpressionYeastMutationMutation I231F. Also insert is missing residues 1…PromoterGAL1Available SinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2374
Plasmid#67534PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked KlURA3 marker, integration into S. cerevisiae XI-1 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK371
Plasmid#73596PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-3) with C-terminal c-myc epitopeDepositorInsertsdelta-12 fatty acid acetylenase
delta-12 fatty acid desaturase (form 3)
TagsFLAG tag and c-myc tagExpressionYeastMutationPoint mutants in FAD2-3 ORF relative to deposited…PromoterGAL1 and GAL10Available SinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK142
Plasmid#73317PurposeEncodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) as a fusion to Pas2p, with c-myc tag and His6-tagDepositorInsertdelta-12 fatty acid acetylenase
TagsHis6, Pas2p, and c-mycExpressionYeastMutationsynthetic gene. Encodes a glycine-204 to serine m…PromoterAOX1Available SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK694
Plasmid#72379PurposeProduces human phosphoribosyl pyrophosphate amidotransferase (PRAT), synthetic geneDepositorAvailable SinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTG005
Plasmid#71386Purposelocalization of yeast RNase RMP1 (RNase MRP binding protein) in yeastDepositorAvailable SinceFeb. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK277
Plasmid#71565PurposeProduces Crepis alpina delta15 fatty acid desaturase (FAD3) in yeastDepositorInsertdelta15 fatty acid desaturase
ExpressionBacterial and YeastPromoterGAL1Available SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK273
Plasmid#71563PurposeProduces Crepis alpina delta12 fatty acid desaturase (FAD2-2) in yeastDepositorInsertdelta12 fatty acid desaturase (form 2)
ExpressionBacterial and YeastPromoterGAL1Available SinceJan. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDS1U-PKS12
Plasmid#70150PurposeFusarium graminearum PKS12 encoded on CEN/ARS yeast shuttle vector with DS1U selection markerDepositorAvailable SinceDec. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP42
Plasmid#65518PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-Cit-attR1 and attR2-mCer-cMycExpressionYeastPromoterpPMA1Available SinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP35
Plasmid#65513PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-AFPt9-attR1 and attR2-TFPt9-cMycExpressionYeastPromoterpPMA1Available SinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
BS-Lys2
Plasmid#69199Purposecomplementation of yeast auxotrophic marker LYS2DepositorInsertLYS2
ExpressionYeastAvailable SinceNov. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
LD121
Plasmid#69172PurposeIntegration of GFP tag at Atg21 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg21-EGFP
TagsEGFPExpressionYeastAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
LD111
Plasmid#69165PurposeIntegration of GFP tag at Atg11 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg11-EGFP
TagsEGFPExpressionYeastAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
LD106
Plasmid#69163PurposeIntegration of GFP tag at Atg6 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg6-EGFP
TagsEGFPExpressionYeastAvailable SinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pED45: pRS306 pRPS2-Ste7DS-NLS-mCherry
Plasmid#68274PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCherryDepositorInsertSte7DS NLS mCherry
TagsmCherryExpressionYeastPromoterpRPS2Available SinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
LD117
Plasmid#69169PurposeIntegration of GFP tag at Atg17 C terminus, use auxotrophic marker URA3(Candida albicans)DepositorInsertAtg17-EGFP
TagsEGFPExpressionYeastAvailable SinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRG227
Plasmid#64532PurposeEpisomal E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP32
Plasmid#65511PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-AFPt9-attR1 and attR2-t7CFPt9-cMycExpressionYeastPromoterpPMA1Available SinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
PSUC2-GFPantisense-CYC1t-pRS413
Plasmid#64404PurposeSUC2 regulated expression of an antisense RNAi construct for GFPDepositorInsertGFPantisense
ExpressionYeastPromoterSUC2Available SinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only