8,126 results

1. pNB788

   Plasmid

   #60165

   Purpose

   Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by RNR1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc-yEVenus
   

   Expression

   Yeast

   Mutation

   FLuc has A4V & S504G (no functional effect)

   Promoter

   RNR1pr

   Available Since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pNB691

   Plasmid

   #60135

   Purpose

   Red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   RdLuc
   

   Tags

   ClpXP degron and Nuclear localization signal (NLS…

   Expression

   Yeast

   Promoter

   MET17pr

   Available Since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 3x_a1gRNAOp_pCYC1m_yeBFP2

   Plasmid

   #64391

   Purpose

   encodes three a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFP

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   yeast enhanced BFP2
   

   Expression

   Yeast

   Available Since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pRG227

   Plasmid

   #64532

   Purpose

   Episomal E. coli/S. cerevisiae shuttle vector

   Depositor

   Joerg Stelling

   Article

   Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAG413GPD-EYFP-ccdB

   Plasmid

   #14334

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EYFP

   Expression

   Yeast

   Available Since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p404GALL

   Plasmid

   #17418

   Depositor

   Nicolas Buchler, Fred Cross

   Insert

   GALL promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   GALL promoter is between SacI and XbaI restrictio…

   Available Since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAG303GAL-ccdB-DsRed

   Plasmid

   #14349

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   DsRed

   Expression

   Yeast

   Available Since

   March 29, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAG306GAL-ccdB-Cerulean

   Plasmid

   #14379

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   Cerulean

   Expression

   Yeast

   Available Since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFA6a-link-yoMaxGFP-Kan

   Plasmid

   #44907

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized MaxGFP with Kan selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoMaxGFP
   

   Expression

   Yeast

   Available Since

   May 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJJH1307

    Plasmid

    #36910

    Depositor

    Jürgen Heinisch

    Article

    Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

    Insert

    ScLEU2
    

    Use

    Lentiviral

    Expression

    Bacterial, Mammalian, and Y…

    Available Since

    July 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNB753

    Plasmid

    #60148

    Purpose

    Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by LEU1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    GrLuc
    

    Tags

    ClpXP degron and Nuclear localization signal (NLS…

    Expression

    Yeast

    Promoter

    LEU1pr

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRPR1_c6gRNA_RPR1t

    Plasmid

    #64384

    Purpose

    encodes c6 gRNA

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    c6 gRNA
    

    Expression

    Yeast

    Available Since

    May 5, 2015

    Availability

    Academic Institutions and Nonprofits only

13. p41Neo 3-F GW

    Plasmid

    #58554

    Purpose

    CEN vector with G418 resistance marker for triple Gateway insertion

    Depositor

    Leonid Kruglyak

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. LexA-Fus3

    Plasmid

    #18899

    Depositor

    Elaine Elion

    Article

    Elion et al Cell. 1990 Feb 23. 60(4):649-64.

    Insert

    FUS3 (FUS3 Budding Yeast)
    

    Expression

    Yeast

    Available Since

    July 27, 2008

    Availability

    Academic Institutions and Nonprofits only

15. Gal4pBS24-Pleum

    Plasmid

    #60327

    Purpose

    Contains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Expression

    Yeast

    Available Since

    Nov. 6, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pRS406-PHO5-GFP-CERT PH domain EE mutation

    Plasmid

    #58833

    Purpose

    Expresses GFP-Tagged and mutated CERT PH domain in yeast

    Depositor

    Tim Levine

    Article

    Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

    Insert

    collagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)
    

    Tags

    GFP and Myc

    Expression

    Yeast

    Mutation

    changed Arginine 43 to Glutamate

    Promoter

    PHO5

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pGLx2-set1deltaRRM

    Plasmid

    #53257

    Purpose

    GAL driven LexA-tagged SET1-deltaRRM (RRM domain deleted)

    Depositor

    Jean Cook

    Article

    Rizzardi et al Genetics. 2012 Oct;192(2):371-84. doi: 1

    Insert

    SET1
    

    Tags

    LexA

    Expression

    Yeast

    Mutation

    RRM domain deleted (aa 230-355)

    Promoter

    GAL1/10

    Available Since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. 413-Met25 Kar2 TorsinADE HDEL

    Plasmid

    #32118

    Depositor

    Susan Lindquist

    Article

    Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

    Insert

    TorsinA (TOR1A Human)
    

    Tags

    HDEL and Kar2

    Expression

    Yeast

    Mutation

    deletion of E at 302 or 303

    Available Since

    Oct. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

19. p414GPD_Hsp82_CycTER

    Plasmid

    #61710

    Purpose

    Yeast plasmid with Hsp82

    Depositor

    Dan Bolon

    Article

    Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

    Insert

    6xHis-Hsp82 (HSP82 Budding Yeast)
    

    Tags

    6xHis

    Expression

    Yeast

    Promoter

    GPD

    Available Since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS424-TUB2 - human TUBB2a Cterminal tail

    Plasmid

    #60396

    Purpose

    Expression of chimeric yeast beta tubulin (TUB2) - human beta2 tubulin Cterminal tail (TUBB2a) using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB2
    

    Expression

    Yeast

    Mutation

    TUB2 amino acids 429 - end, replaced with human T…

    Promoter

    GAL

    Available Since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only