8,388 results
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Plasmid#65451PurposepILGFP3 was designed to test the promoter activity using the yeast enhanced green fluorescent protein (yEGFP) as reporter.DepositorTypeEmpty backboneTagsyEGFP reporterExpressionYeastAvailable SinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only
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pITGFP3
Plasmid#65452PurposepITGFP3 was designed to test the promoter activity using the destabilized yeast enhanced green fluorescent protein (yEGFP-CLN2PEST) as reporter.DepositorTypeEmpty backboneTagsyEGFP-CLN2PEST reporterExpressionYeastAvailable SinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
3x_a1gRNAOp_pCYC1m_yeBFP2
Plasmid#64391Purposeencodes three a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFPDepositorInsertyeast enhanced BFP2
ExpressionYeastAvailable SinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 - human TUBB6 Cterminal tail
Plasmid#60403PurposeExpression of chimeric yeast beta tubulin (TUB2) - human beta5 tubulin Cterminal tail (TUBB6) using a GAL promoterDepositorInsertTUB2
ExpressionYeastMutationTUB2 amino acids 429 - end, replaced with human T…PromoterGALAvailable SinceMay 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRPR1_c6gRNA_RPR1t
Plasmid#64384Purposeencodes c6 gRNADepositorInsertc6 gRNA
ExpressionYeastAvailable SinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRPR1_a1gRNA_RPR1t
Plasmid#64378Purposeencodes a1 gRNADepositorInserta1 gRNA
ExpressionYeastAvailable SinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYC16
Plasmid#63913PurposeRecipient overexpressing vector to construct CFP tagged fusionsDepositorTypeEmpty backboneTagsCFPExpressionYeastPromoterTEF1 (Saccharomyces cerevisiae)Available SinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJW1508
Plasmid#62362PurposepFA6a BirA-mVenus-SSH1::HIS5DepositorInsertBirA-mVenus-Ssh1
TagsBirA-mVenusExpressionYeastPromoterGal1Available SinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJW1516
Plasmid#62386PurposepFA6a Osm1(M1A)-yoEGFP::LEU2DepositorInsertOSM1-EGFP fusion
TagsEGFPExpressionYeastMutationMethionine 1 to AlaninePromoterAdh1pAvailable SinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB785
Plasmid#60164PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by SIC1 promoter.DepositorInsertFLuc-yEVenus-PEST
TagsPEST degronExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterSIC1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB761
Plasmid#60156PurposeFirefly luciferase (Promega) driven by ADE17 promoter.DepositorInsertFLuc
ExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterADE17prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB787
Plasmid#60152PurposeFirefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1 promoter.DepositorInsertFLuc-yEVenus-PEST
TagsPEST degronExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterLEU1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB784
Plasmid#60163PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by SIC1 promoter.DepositorInsertFLuc-yEVenus
ExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterSIC1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB788
Plasmid#60165PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by RNR1 promoter.DepositorInsertFLuc-yEVenus
ExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterRNR1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB789
Plasmid#60166PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.DepositorInsertFLuc-yEVenus-PEST
TagsPEST degronExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterRNR1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJW3.42
Plasmid#62866PurposeYeast two-hybrid bait vector expressing LexA-PPTR-1 fusionDepositorInsertPPTR-1/pLexA-N bait
UseTwo-hybrid bait vectorTagsLexAExpressionYeastAvailable SinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
p414GPD_Hsp82viiB
Plasmid#61709PurposeYeast plasmid with Hsp82 heterodimer construct.DepositorInsertHsp82viiB (HSP82 Budding Yeast)
Tags6xHisExpressionYeastMutationHeteromeric coiled coil inserted after amino acid…PromoterGPD1Available SinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHOHO2_Hsp82viiA
Plasmid#61708PurposeYeast integration plasmid targeted to the HO loci with heterodimeric Hsp82. Digest with NotI prior to addition to yeast.DepositorInsertHsp82viiA (HSP82 Budding Yeast)
UseYeast integration vector targeting inserts to the…Tags6xHisExpressionYeastMutationHeteromeric coiled coil inserted after amino acid…PromoterTEF1Available SinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
p414GPD_Hsp82_CycTER
Plasmid#61710PurposeYeast plasmid with Hsp82DepositorAvailable SinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB753
Plasmid#60148PurposeGreen beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by LEU1 promoter.DepositorInsertGrLuc
TagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastPromoterLEU1prAvailable SinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only