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pWN018

Plasmid

#233818

Purpose

MoClo-YTK; type3B; NS3 systems; NS3-V2

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

NS3-V2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN079

Plasmid

#233819

Purpose

MoClo-YTK; type3B; NS3 systems; NS3a

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

NS3a

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN080

Plasmid

#233820

Purpose

MoClo-YTK; type3B; NS3 systems; Linker-NS3a

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

Linker-NS3a

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN043

Plasmid

#233821

Purpose

MoClo-YTK; type3B; NS3 systems; DNCR2

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

DNCR2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pWN062

Plasmid

#233822

Purpose

MoClo-YTK; type3A; NS3 systems; LexA-NES

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

LexA-NES

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO117

Plasmid

#233823

Purpose

MoClo-YTK; type3; Reporter; mNeonGreen

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pRO176

Plasmid

#233824

Purpose

MoClo-YTK; type3A; Activation domains; NLS-VP48

Depositor

Jeff Hasty

Article

O'Laughlin et al ACS Synth Biol. 2024 Jan 19;13(1):85-102

Insert

NLS-VP48

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO411

Plasmid

#235732

Purpose

Protein expression of ZZ-FL yeast Atg14 in yeast

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

ATG14

Use

Tags

3xTEV-ZZ

Expression

Yeast

Mutation

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO379

Plasmid

#235733

Purpose

Protein expression of VPS34 + VPS15-ZZ + ZZ-VPS30 (all yeast) in yeast

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS34 (VPS34 Budding Yeast)
VPS15 (VPS15 Budding Yeast)
VPS30 (VPS30 Budding Yeast)

Use

Tags

3xTEV-ZZ and ZZ-3xTEV

Expression

Yeast

Mutation

S2A on ScVPS34

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO359

Plasmid

#235750

Purpose

Protein expression of FL ScVPS34 and FL ScVPS15-ZZ

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS34 (VPS34 Budding Yeast)
VPS15 (VPS15 Budding Yeast)

Use

Tags

3xTEV-ZZ

Expression

Yeast

Mutation

S2A, aa 3-887 and T134A, I851R

Promoter

GAL-TDH3

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO734

Plasmid

#235753

Purpose

Protein expression of ScVPS30 and ZZ-ScVPS38

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Inserts

VPS30 (VPS30 Budding Yeast)
VPS38 (VPS38 Budding Yeast)

Use

Tags

ZZ-3xTEV

Expression

Yeast

Mutation

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAPInt-mNeonGreen-G418

Plasmid

#236484

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mNeonGreen and selection on G418.

Depositor

Daniel Lew

Article

Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

Insert

mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO831

Plasmid

#235754

Purpose

Protein expression of ZZ-ScVPS38 (1-212F)

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS38

Use

Tags

ZZ-3xTEV

Expression

Yeast

Mutation

aa 1-212F

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO934

Plasmid

#235766

Purpose

Expression of ScVPS34 delta CBR1 L31-D55-3xFLAG undeer its own promoter

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34 delta CBR1

Use

Tags

3xFLAG

Expression

Yeast

Mutation

aa 31-55 deleted

Promoter

ScVPS34 promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pYO225

Plasmid

#235729

Purpose

Protin expression of FL yeast VPS15 -ZZ in yeast

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS15

Use

Tags

3xTEV-ZZ

Expression

Yeast

Mutation

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO290

Plasmid

#235731

Purpose

Protein expression in FL yeast VPS34 D731N mutant in yeast

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34

Use

Tags

Expression

Yeast

Mutation

S2A, D731N

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO69

Plasmid

#235747

Purpose

Protein expression of FL ScVPS34 in yeast

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34

Use

Tags

Expression

Yeast

Mutation

S2A

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO361

Plasmid

#235748

Purpose

Protein expression of ScVPS34 HELCAT

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS34 HELCAT

Use

Tags

Expression

Yeast

Mutation

aa 268-875

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO227

Plasmid

#235749

Purpose

Protein expression of ZZ-FL ScVPS30

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

VPS30

Use

Tags

ZZ-3xTEV

Expression

Yeast

Mutation

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pYO832

Plasmid

#235755

Purpose

Protein expression of ScVPS30 (1-215E), untagged

Depositor

Roger Williams

Article

Rostislavleva et al Science. 2015 Oct 9;350(6257):aac7365. d

Insert

ScVPS30

Use

Tags

Expression

Yeast

Mutation

aa 1-215E

Promoter

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge