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pAG414GAL-ccdB-HA

Plasmid

#14239

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

3HA

Expression

Yeast

Available Since

March 29, 2007

Availability

Academic Institutions and Nonprofits only

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pKT0139

Plasmid

#8731

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

yECitrine

Tags

Codon optimized linker

Expression

Yeast

Mutation

yEGFP with S65G, V68L, Q69M, S72A, T203Y

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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pILGFP4M

Plasmid

#104508

Purpose

Used to evaluate the expression output of Saccharomyces eubayanus GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SeGAL2 promoter-yEGFP

Expression

Yeast

Available Since

April 27, 2022

Availability

Academic Institutions and Nonprofits only

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pFA CRU HISMX

Plasmid

#131164

Purpose

PCR template for genomic C-terminal Cub-R-URA3 tag, HISMX complements the histidine auxotrophy of S. cerevisiae his mutant

Depositor

Nils Johnsson

Article

Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

Type

Empty backbone

Tags

CRU

Expression

Yeast

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pFA NuiHA-loxP-nat-CUP1p

Plasmid

#131170

Purpose

PCR template for genomic N-terminal Nui-HA tag, nat-CUP1p flanking loxP sites allow expression by the native promoter after CRE-recombinase expression, nat marker confers resistance to Nourseothricin

Depositor

Nils Johnsson

Article

Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

Type

Empty backbone

Tags

Nui-HA

Expression

Yeast

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pGBW-m4046348

Plasmid

#149108

Purpose

Yeast Expression plasmid for SARS-CoV-2 nsp6

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 nsp6 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable thrombin;3xFLAG

Expression

Yeast

Mutation

Saccharomyces cerevisiae recode 1

Promoter

pGAL1

Available Since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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BS-Leu2

Plasmid

#69200

Purpose

complementation of yeast auxotrophic marker LEU2

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

LEU2

Expression

Yeast

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pDDGFP-Leu2d-GgKDELR2

Plasmid

#123618

Purpose

Expresses WT GgKDELR2 in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

Depositor

Simon Newstead

Article

Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

Insert

KDELR2 (KDELR2 Chicken)

Tags

TEV-GFP-His

Expression

Yeast

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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YIplac211-ANP1-msGFP2

Plasmid

#177100

Purpose

Expresses Anp1-msGFP2 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

ANP1 (ANP1 Budding Yeast)

Expression

Yeast

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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ZJOM2

Plasmid

#133633

Purpose

Integration of VRG4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast early golgi.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-VRG4-GFP (VRG4 Budding Yeast)

Tags

EGFP

Expression

Yeast

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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