8,060 results

1. pAG415Gal_Gcn4 WT 30mer

   Plasmid

   #232986

   Purpose

   Galactose iduced expression of Gcn4 WT 30mer in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 WT 30mer
   

   Use

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   Promoter

   Gal1

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRS424 Prp8 WT TRP1

   Plasmid

   #234120

   Purpose

   The N-terminus of Prp8 was restored thereby destroying the NheI site and correcting the ORF

   Depositor

   Aaron Hoskins

   Insert

   Prp8
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pML107-ARB1

   Plasmid

   #232888

   Purpose

   Plasmid expressing Cas9 and gRNA CAAAAACTAACTGCTTACGG which targets the ARB1 gene.

   Depositor

   Jolanda Van Leeuwen

   Insert

   ARB1 gRNA (ARB1 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   snR52

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFA6a-FLAG-APEX2-NES-HIS3

   Plasmid

   #229230

   Purpose

   Template plasmid for the endogenous tagging with FLAG-APEX2-NAS in yeast

   Depositor

   Fulvio Reggiori

   Article

   Filali-Mouncef et al Autophagy. 2024 Oct;20(10):2323-2337. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRS413-CUP1pr-NFAPoptim

   Plasmid

   #221110

   Purpose

   FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRS413-CUP1pr-CFAPoptim

   Plasmid

   #221111

   Purpose

   FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRS413-TEF1pr-ANP1-FAPoptim

   Plasmid

   #221120

   Purpose

   FAP-tagged marker for the cis-Golgi (FAP optimized for yeast expression)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Insert

   ANP1-FAPoptim
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRS413-TEF1pr-ERG6-FAPoptim

   Plasmid

   #221121

   Purpose

   FAP-tagged marker for lipid droplets (FAP optimized for yeast expression)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Insert

   ERG6-FAPoptim
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRS413-TEF1pr-PMA1-FAPoptim

   Plasmid

   #221122

   Purpose

   FAP-tagged marker for the plasma membrane (FAP optimized for yeast expression)

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Insert

   PMA1-FAPoptim
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRS413-TEF1pr-RPA34-FAPoptim

    Plasmid

    #221123

    Purpose

    FAP-tagged marker for the nucleus (FAP optimized for yeast expression)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    RPA34-FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRS413-TEF1pr-SEC7-FAPoptim

    Plasmid

    #221124

    Purpose

    FAP-tagged marker for the trans-Golgi (FAP optimized for yeast expression)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    SEC7-FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRS413-TEF1pr-VPH1-FAPoptim

    Plasmid

    #221125

    Purpose

    FAP-tagged marker for the vacuolar membrane (FAP optimized for yeast expression)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Insert

    VPH1-FAPoptim
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRS415-TEF1pr-NFAPoptim

    Plasmid

    #221088

    Purpose

    FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRS415-TEF1pr-CFAPoptim

    Plasmid

    #221089

    Purpose

    FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRS415-TEF1pr-MFA-NFAPoptim

    Plasmid

    #221090

    Purpose

    FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ER

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS413-TEF1pr-NFAPorig

    Plasmid

    #221091

    Purpose

    FAP insert for N-terminally tagging genes of interest (original FAP + 2xMYC tag)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRS413-TEF1pr-NFAPoptim

    Plasmid

    #221093

    Purpose

    FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRS413-TEF1pr-CFAPoptim

    Plasmid

    #221094

    Purpose

    FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRS415-ADH1pr-NFAPoptim

    Plasmid

    #221096

    Purpose

    FAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRS415-ADH1pr-CFAPoptim

    Plasmid

    #221097

    Purpose

    FAP insert for C-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag)

    Depositor

    Allyson O’Donnell

    Article

    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge