8,086 results

1. pTO136_pADH1-tRNA-Ala-gRNA-tracrRNA-HDV-tAgTEF2

   Plasmid

   #171104

   Purpose

   Expresses sgRNA (ENO1 gRNA) in Candida auris.

   Depositor

   Teresa O'Meara

   Article

   Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

   Insert

   sgRNA
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available Since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS410

   Plasmid

   #11258

   Depositor

   Fred Cross

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFA6a-6xGLY-Strep-tagII-hphMX4

   Plasmid

   #20758

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDRF1-GW yAT1.03R122KR126K

   Plasmid

   #132782

   Purpose

   Non-binding version of the FRET biosensor for ATP measurements in budding yeast

   Depositor

   Bas Teusink

   Article

   Botman et al ACS Sens. 2020 Mar 27;5(3):814-822. doi:

   Insert

   yAT1.03R122KR126K ymTq2-tdTomato
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   R122K, R126K

   Promoter

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPRyl_AXP

   Plasmid

   #84608

   Purpose

   Introduces DSB at AXP locus in Y. lipolytica

   Depositor

   Ian Wheeldon

   Article

   Schwartz et al ACS Synth Biol. 2016 Dec 19.

   Insert

   Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   UAS1B8-TEF(136)

   Available Since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRS416 Gal TDP43 WT

   Plasmid

   #27458

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Jan. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pFA6a-mNeonGreen-KanMX6

   Plasmid

   #129099

   Purpose

   For tagging genes at 3'end (C-terminal end of protein) in S. cerevisiae with mNeonGreen

   Depositor

   Wei-Lih Lee

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTO135_pENO1-CaCas9-tCyc1

   Plasmid

   #171103

   Purpose

   Expresses Cas9 in Candida auris

   Depositor

   Teresa O'Meara

   Article

   Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

   Insert

   CaCas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ENO1

   Available Since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. YIplac211-ABP1-iGFPx6

   Plasmid

   #177099

   Purpose

   Expresses Abp1-iGFPx6 in yeast cells

   Depositor

   Benjamin Glick

   Article

   Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

   Insert

   ABP1 (ABP1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCdOpt-BMX

    Plasmid

    #203929

    Purpose

    This plasmid contains a codon optimized BleMX drug resistance cassette for generating gene deletions in C. glabrata, C. auris, C. albicans or S. cerevisiae

    Depositor

    Scott Briggs

    Article

    Gregor et al mSphere. 2023 Nov 6:e0031123. doi: 10.11

    Insert

    BleMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    BleMX was codon optimized for Candida albicans or…

    Promoter

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFA6a-5FLAG-natMX6

    Plasmid

    #19343

    Depositor

    Eishi Noguchi

    Article

    Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

    Insert

    natMX6
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. EZ-L560

    Plasmid

    #173028

    Purpose

    Encodes OptoAMP2 circuit

    Depositor

    Jose Avalos

    Article

    Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAR-Ec611

    Plasmid

    #130872

    Purpose

    A nuclear plasmid encoding an error-prone mutant TP-DNAP1 (I777K, L900S) for OrthoRep

    Depositor

    Chang Liu

    Article

    Ravikumar et al Cell. 2018 Dec 13;175(7):1946-1957.e13.

    Insert

    TP-DNAP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    I777K, L900S

    Promoter

    Available Since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVS2

    Plasmid

    #33144

    Depositor

    Michael Rosbash

    Article

    Vodala et al Mol Cell. 2008 Jul 11;31(1):104-13.

    Inserts

    LacO (256 copies)
    GAL-GFP-kd200-GALpA reporter
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    GFP is mutated such that amino acids 64-73 (FSYGV…

    Promoter

    bidirectional GAL1-GAL10 promoter

    Available Since

    Dec. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

15. pCAS9i

    Plasmid

    #141249

    Purpose

    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Article

    Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1 (Cas9), pSNR52 (gRNA)

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA

    Plasmid

    #73796

    Purpose

    pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA

    Depositor

    Ronald Davis

    Article

    Smith et al Genome Biol. 2016 Mar 8;17(1):45. doi: 1

    Inserts

    dCas9-Mxi1
    Tet Repressor
    Structural gRNA for S pyogenes
    

    Use

    CRISPR

    Tags

    Mxi1 and NLS

    Expression

    Yeast

    Mutation

    D10A, H840A

    Promoter

    pGPM1, pRPR1(TetO), and pTef1

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSAP

    Plasmid

    #206429

    Purpose

    A vector backbone domesticated for SapI (pCC1BAC-based), contains elements for selection, maintenance, and propagation in S. cerevisiae (TRP1) and E. coli (cat).

    Depositor

    Bogumil Karas

    Article

    Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRS426GFP-2×PH(PLCδ)

    Plasmid

    #36092

    Depositor

    Scott Emr

    Article

    Stefan et al Mol Biol Cell. 2002 Feb;13(2):542-57.

    Insert

    2xPH(PLCdelta) fusion
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    contains aa11-140 of PLCδ (including the PH domai…

    Promoter

    PRC1

    Available Since

    June 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAPInt-sfGFP-G418

    Plasmid

    #236481

    Purpose

    Plasmid for deleting or C-terminally tagging endogenous genes with sfGFP and selection on G418.

    Depositor

    Daniel Lew

    Article

    Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Codon optimized for A. pullulans

    Promoter

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGI3EM11

    Plasmid

    #135483

    Purpose

    Agrobacterium T-DNA with SpunH2Bpr driving hph-GFP

    Depositor

    Nicolas Buchler

    Article

    Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

    Insert

    SpunH2Bpr hph-GFP ADH1t
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpunH2Bpr

    Available Since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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