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pFA6a-link-ymScarletI3-URA3

Plasmid

#224075

Purpose

Yeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with URA3 selection

Depositor

Bas Teusink

Insert

ymScarletI3

Tags

ymScarletI3

Expression

Yeast

Available Since

Feb. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pPM28 (eroGFP CEN/ARS URA3)

Plasmid

#20131

Depositor

Feroz Papa

Article

Merksamer et al Cell. 2008 Nov 28. 135(5):933-47.

Insert

eroGFP (KAR2 jellyfish, Budding Yeast)

Expression

Yeast

Available Since

Feb. 11, 2009

Availability

Academic Institutions and Nonprofits only

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pEasyCas9

Plasmid

#184909

Purpose

Expresses the Streptococcus pyogenes Cas9 protein

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

Cas9

Use

CRISPR

Expression

Bacterial and Yeast

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pRM200U

Plasmid

#44529

Depositor

Michael Grunstein

Article

Mann et al EMBO J. 1992 Sep;11(9):3297-306.

Insert

HHF2-HHT2

Expression

Yeast

Available Since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pfa6a mScarlet-FKBP::KANMX

Plasmid

#185905

Purpose

Plasmid for cloning mScarlet fused to FKBP into yeast.

Depositor

Matthew Good

Article

Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

Insert

mScarlet-FKBP

Tags

mScarlet fused to FKBP

Expression

Yeast

Promoter

SP6

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pESC-MET

Plasmid

#222482

Purpose

Co-expression of two genes in yeast with FLAG and MYC tags and MET15 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

FLAG and Myc

Expression

Yeast

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pESC-LYS

Plasmid

#222483

Purpose

Co-expression of two genes in yeast with FLAG and MYC tags and LYS2 selection

Depositor

Tetsuo Matsuzaki, Kiyofumi Yamada

Article

Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

Type

Empty backbone

Tags

FLAG and Myc

Expression

Yeast

Available Since

Oct. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pTDH3-dCas9-Mxi1

Plasmid

#46921

Purpose

Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS and Mxi1 domain controlled by TDH3 promoter

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

dCas9-Mxi1

Use

CRISPR

Expression

Yeast

Promoter

TDH3

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pYX122-mtGFP

Plasmid

#45048

Depositor

Benedikt Westermann

Article

Westermann et al Yeast. 2000 Nov;16(15):1421-7.

Insert

mtGFP

Expression

Yeast

Promoter

TPI

Available Since

May 17, 2013

Availability

Academic Institutions and Nonprofits only
pPICZ-pre-pro-alphaf(I)-E2-Crimson

Plasmid

#117660

Purpose

Test intracellular localisation of E2-Crimson and study the secretion efficiency

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pre-pro-alphaf(I)-E2-Crimson

Expression

Yeast

Mutation

This plasmid encodes the fluorescent protein E2-C…

Promoter

pAOX1

Available Since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCAP05

Plasmid

#102920

Purpose

Direct cloning and broad-host-range heterologous expression vector

Depositor

Bradley Moore

Article

Zhang et al MBio. 2017 Sep 5;8(5). pii: e01291-17. d

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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Gal:mCherry-CRY2-uTEV1Δ(220-242)

Plasmid

#135452

Purpose

Yeast expression of uTEV1Δ fused to CRY2 and mCherry

Depositor

Alice Ting

Article

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

mCherry-CRY2-TEV1Δ(220-242)

Tags

mCherry

Expression

Yeast

Promoter

Gal

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-GBP-RFP-kanMX6

Plasmid

#105160

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Article

Davidson et al Mol Biol Cell. 2015 Feb 1;26(3):453-66.

Insert

GBP-RFP-kanMX6

Tags

GBP-RFP

Expression

Bacterial and Yeast

Available Since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pJKW1148

Plasmid

#119029

Purpose

Yeast expression plasmid for PmCYP719A26

Depositor

Jing-Ke Weng

Article

Pluskal et al Nat Plants. 2019 Jul 22. pii: 10.1038/s4

Insert

PmCYP719A26

Expression

Yeast

Promoter

GAL10

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pNTI647 dCas9-Mxi1 TetR KanMX

Plasmid

#139474

Purpose

Integrates CRISPRi effector and tet-responsive regulator

Depositor

Nicholas Ingolia

Article

McGlincy et al BMC Genomics. 2021 Mar 23;22(1):205. doi

Inserts

dCas9-Mxi1
Tet Repressor

Use

CRISPR

Tags

Mxi1 and NLS

Expression

Yeast

Mutation

D10A, H840A

Promoter

pGPM1 and pTEF1

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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YIplac211-yoHaloTag-TLG1

Plasmid

#105277

Purpose

Plasmid for pop-in/pop-out replacement of Tlg1 with yoHaloTag-Tlg1.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Tlg1 (TLG1 Budding Yeast)

Tags

yoHaloTag

Expression

Yeast

Mutation

HaloTag has yeast optimized codons

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pDS240

Plasmid

#34966

Purpose

Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep(K533∆, high-affinity epitope).

Depositor

Michael Glotzer

Article

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Mid2(SS/TM)-GFP-LOVpep

Expression

Yeast

Mutation

K533∆, high-affinity epitope

Promoter

GAL1

Available Since

March 21, 2012

Availability

Academic Institutions and Nonprofits only

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p306-BrdU-Inc

Plasmid

#71792

Purpose

BrdU-incorporating plasmid with selectable marker URA3

Depositor

Oscar Aparicio

Article

Viggiani et al Yeast. 2006 Oct-Nov;23(14-15):1045-51.

Inserts

Thymidine Kinase
ENT1 (SLC29A1 Human)

Expression

Yeast

Promoter

ADH1 and GPD

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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YL-CashGem-BAT1-URA3-LINEAR

Plasmid

#174837

Purpose

pRS414 backbone containing LINEAR fragment targeting BAT1 in Y. lipolytica

Depositor

Zengyi Shao

Article

Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

Insert

Codon optimized Cas9:hGem

Use

CRISPR and Synthetic Biology

Tags

hGeminin tag

Expression

Bacterial and Yeast

Promoter

GPDp

Available Since

Jan. 5, 2022

Availability

Academic Institutions and Nonprofits only

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P1K-3HA-Atg8∆R-MET15

Plasmid

#207034

Purpose

Expression of 3HA-Atg8∆R. This variant bypasses the need of Atg4 for initial C-terminal processing.

Depositor

Zhiping Xie

Article

He et al BMC Biol. 2021 Jun 4;19(1):117. doi: 10.

Insert

ATG8

Tags

3xHA

Expression

Yeast

Mutation

∆R

Promoter

pATG8

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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