8,126 results

1. pFA6a-BirA-HA3-KanMX6

   Plasmid

   #227974

   Purpose

   To insert HA-tagged biotin ligase, BirA, at the C-terminus of genes; yeast BioID with KanMX6 marker

   Depositor

   Joseph Reese

   Article

   Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

   Insert

   BirA
   

   Tags

   HA tag

   Expression

   Yeast

   Available Since

   Nov. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CPH3375_SF_Spt6p-tSH2-1223-1451_NLS

   Plasmid

   #227084

   Purpose

   Gal1p dependent budding yeast expression of tandem-affinity tagged spt6p tSH2 domain (1123-1451) localized to nucleus

   Depositor

   Chris Hill

   Insert

   spt6-tsh2 (SPT6 Budding Yeast)
   

   Tags

   STREPii-FLAG and SV40 NLS 1

   Expression

   Yeast

   Promoter

   Gal1-10

   Available Since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. CUP1-∆ssCPY*-mScarletI

   Plasmid

   #221157

   Purpose

   Fluorescent reporter for cytoplasmic aggregates

   Depositor

   Mark Leake

   Insert

   Carboxypeptidase Y (PRC1 Budding Yeast)
   

   Tags

   mScarletI

   Expression

   Yeast

   Mutation

   Deletion signal peptide, G255A mutation

   Available Since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CUP1-∆ssCPY*-mNeonGreen

   Plasmid

   #221156

   Purpose

   Fluorescent reporter for cytoplasmic aggregates

   Depositor

   Mark Leake

   Insert

   Carboxypeptidase Y (PRC1 Budding Yeast)
   

   Tags

   mNeonGreen

   Expression

   Yeast

   Mutation

   Deletion signal peptide, G255A mutation

   Available Since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CUP1-∆ssCPY*-mNeonGreen in pRS315

   Plasmid

   #221160

   Purpose

   Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker

   Depositor

   Mark Leake

   Insert

   Carboxypeptidase Y (PRC1 Budding Yeast)
   

   Tags

   mNeonGreen

   Expression

   Yeast

   Mutation

   Deletion signal peptide, G255A mutation

   Available Since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CUP1-∆ssCPY*-mGFP

   Plasmid

   #212197

   Purpose

   Fluorescent reporter for cytoplasmic aggregates

   Depositor

   Mark Leake, Chris MacDonald

   Insert

   Carboxypeptidase Y variant (PRC1 Budding Yeast)
   

   Tags

   mEGFP (monomeric version EGFP, A206K mutation)

   Expression

   Yeast

   Mutation

   Deletion signal peptide, G255A mutation

   Promoter

   CUP1

   Available Since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPOM034_P7_3"Lys3

   Plasmid

   #216459

   Purpose

   3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.

   Depositor

   Tomáš Pluskal

   Article

   Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

   Insert

   3''lys3
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Mutation

   Removal of any BsaI // BsmBI restriction site

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRSII41K

   Plasmid

   #194522

   Purpose

   Low copy vector backbone with G418 selectivity, contains MCS

   Depositor

   Patrick Gibney

   Article

   Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRSII41N

   Plasmid

   #194524

   Purpose

   Low copy vector backbone with nourseothricin selectivity, contains MCS

   Depositor

   Patrick Gibney

   Article

   Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRSII42N

    Plasmid

    #194525

    Purpose

    High copy vector backbone with nourseothricin selectivity, contains MCS

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRSII41H

    Plasmid

    #194526

    Purpose

    Low copy vector backbone with hygromycin B selectivity, contains MCS

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRSII42H

    Plasmid

    #194527

    Purpose

    High copy vector backbone with hygromycin B selectivity, contains MCS

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRSII41K-TDH3pr

    Plasmid

    #194528

    Purpose

    Low copy vector backbone with G418 selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRSII42K-TDH3pr

    Plasmid

    #194529

    Purpose

    High copy vector backbone with G418 selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRSII41N-TDH3pr

    Plasmid

    #194530

    Purpose

    Low copy vector backbone with nourseothricin selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRSII41H-TDH3pr

    Plasmid

    #194532

    Purpose

    Low copy vector backbone with hygromycin B selectivity, contains TDH3(pr)-MCS-CYC1(3'UTR)

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRSII41K-TDH3pr-mNeon

    Plasmid

    #194534

    Purpose

    Low copy vector backbone with G418 selectivity, encodes for TDH3(pr) driven expression of mNeon

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Insert

    kanMX
    

    Expression

    Yeast

    Promoter

    TDH3

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRSII42K-TDH3pr-mNeon

    Plasmid

    #194535

    Purpose

    High copy vector backbone with G418 selectivity, encodes for TDH3(pr) driven expression of mNeon

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Insert

    kanMX
    

    Expression

    Yeast

    Promoter

    TDH3

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRSII41N-TDH3pr-mNeon

    Plasmid

    #194536

    Purpose

    Low copy vector backbone with nourseothricin selectivity, encodes for TDH3(pr) driven expression of mNeon

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Insert

    natAC
    

    Expression

    Yeast

    Promoter

    TDH3

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSII42N-TDH3pr-mNeon

    Plasmid

    #194537

    Purpose

    High copy vector backbone with nourseothricin selectivity, encodes for TDH3(pr) driven expression of mNeon

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Insert

    natAC
    

    Expression

    Yeast

    Promoter

    TDH3

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge