8,126 results

1. pSCL.002

   Plasmid

   #184972

   Purpose

   Express -Eco1 ADE2 editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 12
   

   Expression

   Yeast

   Mutation

   ADE2 donor P272stop

   Promoter

   Gal7

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCL.110

   Plasmid

   #184979

   Purpose

   Express -Eco1 TRP2 editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 12
   

   Expression

   Yeast

   Mutation

   TRP2 donor E64stop

   Promoter

   Gal7

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSCL.039

   Plasmid

   #184973

   Purpose

   Test effect of extending a1/a2 on ADE2 editing rates in yeast

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v1
   

   Expression

   Yeast

   Mutation

   ADE2 donor P272stop, a1/a2 length extended to 27 …

   Promoter

   Gal7

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Yeast_Target-AID2S

   Plasmid

   #188647

   Purpose

   Expressing Target-AID2S in yeasts

   Depositor

   Keiji Nishida

   Article

   Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

   Insert

   nCas9-AID2S-UGI
   

   Tags

   UGI

   Expression

   Yeast

   Mutation

   D10A for SpCas9

   Promoter

   pGal1

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pM-mRuby2

   Plasmid

   #176550

   Purpose

   Expression of mRuby2 for auxotrophic selection in the absence of methionine

   Depositor

   Markus Ralser

   Article

   Yu et al Nat Microbiol. 2022 Apr;7(4):542-555. do

   Insert

   yomRuby2
   

   Expression

   Yeast

   Promoter

   TDH1(GAP)

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pH-mWasabi

   Plasmid

   #176551

   Purpose

   Expression of mWasabi for auxotrophic selection in the absence of histidine

   Depositor

   Markus Ralser

   Insert

   mWasabi
   

   Expression

   Yeast

   Promoter

   TDH1(GAP)

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pU-mWasabi

   Plasmid

   #176553

   Purpose

   Expression of mWasabi for auxotrophic selection in the absence of uracil

   Depositor

   Markus Ralser

   Insert

   mWasabi
   

   Expression

   Yeast

   Promoter

   TDH1(GAP)

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pM-mWasabi

   Plasmid

   #176554

   Purpose

   Expression of mWasabi for auxotrophic selection in the absence of methionine

   Depositor

   Markus Ralser

   Insert

   mWasabi
   

   Expression

   Yeast

   Promoter

   TDH1(GAP)

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pU-TagRFP657

   Plasmid

   #176562

   Purpose

   Expression of TagRFP657 for auxotrophic selection in the absence of uracil

   Depositor

   Markus Ralser

   Insert

   TagRFP657
   

   Expression

   Yeast

   Promoter

   TDH1(GAP)

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pU-YLL058W-lt

    Plasmid

    #176564

    Purpose

    Expression of YLL058W for Lanchea thermotolerans for auxotrophic selection in the absence of uracil

    Depositor

    Markus Ralser

    Insert

    YLL058W-lt
    

    Expression

    Yeast

    Promoter

    TDH1(GAP)

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pU-YLL058W-zr

    Plasmid

    #176565

    Purpose

    Expression of YLL058W from Zygosaccharomyces rouxii for auxotrophic selection in the absence of uracil

    Depositor

    Markus Ralser

    Insert

    YLL058W-zr
    

    Expression

    Yeast

    Mutation

    Codon optimised for expression in S.cerevisae

    Promoter

    TDH1(GAP)

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pH-Sapphire

    Plasmid

    #176566

    Purpose

    Expression of Sapphire for auxotrophic selection in the absence of lysine

    Depositor

    Markus Ralser

    Insert

    yETSapphire
    

    Expression

    Yeast

    Promoter

    TDH1(GAP)

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU-Sapphire

    Plasmid

    #176567

    Purpose

    Expression of Sapphire for auxotrophic selection in the absence of uracil

    Depositor

    Markus Ralser

    Insert

    yETSapphire
    

    Expression

    Yeast

    Promoter

    TDH1(GAP)

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pM-Sapphire

    Plasmid

    #176569

    Purpose

    Expression of Sapphire for auxotrophic selection in the absence of methionine

    Depositor

    Markus Ralser

    Insert

    yETSapphire
    

    Expression

    Yeast

    Promoter

    TDH1(GAP)

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LDB73

    Plasmid

    #185437

    Purpose

    NUP1 CEN URA3 vector for synthetic lethal sectoring assays and screening

    Depositor

    Laura Davis, Ken Belanger

    Article

    Belanger et al J Cell Biol. 1994 Aug;126(3):619-30. doi

    Insert

    NUP1
    

    Expression

    Yeast

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pIFEX-A2aGH

    Plasmid

    #160542

    Purpose

    pITY4 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

    Depositor

    Michelle O'Malley

    Article

    Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

    Insert

    Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)
    

    Expression

    Yeast

    Mutation

    N/A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEFEX1-A2aGH

    Plasmid

    #160543

    Purpose

    pYES2 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

    Depositor

    Michelle O'Malley

    Article

    Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

    Insert

    Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)
    

    Expression

    Yeast

    Mutation

    N/A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEFEX2-A2aGH

    Plasmid

    #160544

    Purpose

    pYES2 derivative reporter plasmid encoding PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

    Depositor

    Michelle O'Malley

    Article

    Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

    Insert

    PPGI1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)
    

    Expression

    Yeast

    Mutation

    N/A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEFEX3-A2aGH

    Plasmid

    #160545

    Purpose

    pYES2 derivative reporter plasmid encoding PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

    Depositor

    Michelle O'Malley

    Article

    Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

    Insert

    PREV1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)
    

    Expression

    Yeast

    Mutation

    N/A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCFEX1-A2aGH

    Plasmid

    #160546

    Purpose

    pYC2/CT derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

    Depositor

    Michelle O'Malley

    Article

    Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

    Insert

    Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Human, Budding Yeast)
    

    Expression

    Yeast

    Mutation

    N/A

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge