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pEM.P01R

Plasmid

#198642

Purpose

Easy-MISE toolkit pEM-plasmid containing TDH3 promoter with FG protruding ends

Depositor

Paola Branduardi

Publication

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

pTDH3

Use

Synthetic Biology

Expression

Yeast

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pEM.P01L

Plasmid

#198641

Purpose

Easy-MISE toolkit pEM-plasmid containing TDH3 promoter with EF protruding ends

Depositor

Paola Branduardi

Publication

Maestroni et al ACS Synth Biol. 2023 Apr 14. doi: 10.102

Insert

pTDH3

Use

Synthetic Biology

Expression

Yeast

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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Aga2p-LOV-Turbo1-myc_pCTCON2

Plasmid

#199673

Purpose

expresses pre-evolved version of LOV-Turbo on the yeast surface

Depositor

Alice Ting

Publication

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo1

Tags

Aga2p and Myc

Expression

Yeast

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-FHIP

Plasmid

#198527

Purpose

Expression of GAL4 DNA-binding domain (BD)-FHIP fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Publication

Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

Insert

FHIP (FHIP1B Human)

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Mutation

silent mutations in codons 353 and 651 as well as…

Promoter

ADH1

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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LT2_15_UPRpro_X2site

Plasmid

#194427

Purpose

Plasmid for genome integration in X2 site expressing UPRpro - Sensor for Unfolded Protein Response

Depositor

Lisbeth Olsson

Publication

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Insert

UPRpro

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Mutation

NA

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT2_32_PyruEth_X2site

Plasmid

#194428

Purpose

Plasmid for genome integration in X2 site expressing PyruEth (Pyruvate and Ethanol consumption sensors)

Depositor

Lisbeth Olsson

Publication

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Insert

PyruEth

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Mutation

NA

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT2_33_PyruPro_X2site

Plasmid

#194429

Purpose

Plasmid for genome integration in X2 site expressing PyruPro (Pyruvate consumption Probe)

Depositor

Lisbeth Olsson

Publication

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Insert

PyruPro

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Mutation

NA

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT1_75_Donor_Tag_RPL13A-mTurq2

Plasmid

#194415

Purpose

Plasmid for genome integration of mTurquoise2 CDS to tag native RPL13A for biosensors RibPro and UPRpro

Depositor

Lisbeth Olsson

Publication

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Insert

mTurquoise2 CDS

Use

Synthetic Biology; Used as donor dna in genome in…

Expression

Yeast

Mutation

NA

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pAG306-pTet07-leuYFP[CTT]-nLuc

Plasmid

#199757

Purpose

Elongation reporter construct to quantify elongation of synYFP[CTT]

Depositor

Brian Zid

Publication

Hou et al RNA. 2023 Dec;29(12):1928-1938. doi: 10.

Insert

leuYFP[CTT]-nLuc

Expression

Yeast

Promoter

TetO7

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAG306-pTet07-leuYFP[CTC]-nLuc

Plasmid

#199758

Purpose

Elongation reporter construct to quantify elongation of synYFP[CTC]

Depositor

Brian Zid

Publication

Hou et al RNA. 2023 Dec;29(12):1928-1938. doi: 10.

Insert

leuYFP[CTC]

Expression

Yeast

Promoter

TetO7

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAG306-pTet07-synYFP[TTG/AGA]-6xArg[CGA]-nLuc

Plasmid

#199760

Purpose

Elongation reporter construct to quantify elongation duration of 6_CGA stalling

Depositor

Brian Zid

Publication

Hou et al RNA. 2023 Dec;29(12):1928-1938. doi: 10.

Insert

synYFP[TTG/AGA]-6xArg[CGA]-nLuc

Expression

Yeast

Promoter

TetO7

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAG306-pTet07-synYFP[TTG/AGA]-6xArg[AGA]-nLuc

Plasmid

#199761

Purpose

Elongation reporter construct to quantify elongation duration of 6_AGA stalling

Depositor

Brian Zid

Publication

Hou et al RNA. 2023 Dec;29(12):1928-1938. doi: 10.

Insert

synYFP[TTG/AGA]-6xArg[AGA]-cLuc

Expression

Yeast

Promoter

TetO7

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT 6FLAG

Plasmid

#182509

Purpose

C-SWAT compatible vector for C-terminal tagging with 6 FLAG

Depositor

Rob Mitra

Publication

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

6 FLAG

Expression

Yeast

Available since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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C-SWAT IAA17-6FLAG (no NLS)

Plasmid

#193322

Purpose

C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration and does not have NLS tags..

Depositor

Rob Mitra

Publication

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

IAA17

Tags

6FLAG

Expression

Yeast

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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M2C5

Plasmid

#188606

Purpose

Minimal Conjugative Plasmid 2

Depositor

Bogumil Karas

Publication

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Diatom expression

Expression

Bacterial and Yeast

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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M1C1

Plasmid

#188609

Purpose

Minimal Conjugative Plasmid 1

Depositor

Bogumil Karas

Publication

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology; Diatom expression

Expression

Bacterial and Yeast

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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M4C16

Plasmid

#188610

Purpose

Minimal Conjugative Plasmid 4

Depositor

Bogumil Karas

Publication

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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M4C19

Plasmid

#188611

Purpose

Minimal Conjugative Plasmid 4

Depositor

Bogumil Karas

Publication

Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pACT2-μ4

Plasmid

#198176

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-4 μ4 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Publication

Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

Insert

AP-4 μ4

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Promoter

ADH1

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGBT9-GGA2-GAE

Plasmid

#199175

Purpose

Expression of GAL4 DNA-binding domain (BD)-GGA2-GAE domain fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Publication

Mattera et al EMBO J. 2003 Jan 2;22(1):78-88. doi: 10.

Insert

GGA2-GAE domain (GGA2 Human)

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Mutation

Contains Lys 545 Gln substitution

Promoter

ADH1

Available since

April 10, 2023

Availability

Academic Institutions and Nonprofits only

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