8,418 results
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Plasmid#196834PurposeExpresses Pab1 RRM2 fragment fused to GAL4 DNA binding domainDepositorInsertPab1 (PAB1 Budding Yeast)
TagsGal4ExpressionYeastMutation123-204 amino acidsPromoterADHAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOBD2 Pab1 E181R (123-204)
Plasmid#196835PurposeExpresses Pab1 RRM2 fragment E181R fused to GAL4 DNA binding domainDepositorInsertPab1 (PAB1 Budding Yeast)
TagsGal4ExpressionYeastMutation123-204 amino acids & E181RPromoterADHAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROS13-Tps2/Gsy1
Plasmid#196613PurposeEncoding guide RNAs for the knock out of TPS2 and GSY1 genesDepositorAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-γ1
Plasmid#197078PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-1 γ1 fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)DepositorInsertAP-1 γ1 (Ap1g1 Mouse)
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastPromoterADH1Available sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-ε
Plasmid#197261PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-4 ε fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)DepositorInsertAP-4 ε (AP4E1 Human)
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationsilent substitutions in codons 905, 1129 and 1137PromoterADH1Available sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-tepsin
Plasmid#196647PurposeExpression of GAL4 DNA-binding domain (BD)-tepsin fusion proteinDepositorInserttepsin (TEPSIN Human)
UseYeast two-hybrid vectorTagsGAL4-DNA binding domain fragmentExpressionYeastMutationsilent substitution in codon 199 to eliminate int…Available sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
GA46
Plasmid#196614PurposePlasmid used for the C-terminal tagging of Atp3 with mCherry and the auxin-inducible degron in yeastDepositorInsertATP3::mCherry-AID(71-114)-NatMX (ATP3 Budding Yeast)
TagsAID(71-114) and mCherryExpressionYeastAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUGP1.1
Plasmid#196615PurposePlasmid used for the C-terminal tagging of Ugp1 with mCherry and the auxin-inducible degron in yeastDepositorInsertUGP1::mCherry-AID(71-114)-NatMX (UGP1 Budding Yeast)
TagsAID(71-114) and mCherryExpressionYeastPromoterNative promoterAvailable sinceMarch 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAS-mak10 gRNA-MAK10Δ
Plasmid#160384PurposeFor CRISPR-mediated deletin og MAK10 in yeastDepositorInsertMAK10
ExpressionYeastPromoterPGK1Available sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAS-mak10 gRNA
Plasmid#160385PurposeExpresses guide RNA for targeting MAK10 in yeastDepositorInsertmak10 gRNA
ExpressionYeastPromoterRNA pol III promoter (tRNA-Tyr)Available sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
OM14-2EGFP-UG74-ter
Plasmid#184758PurposeMarker for yeast mitochondria. Integration of 2xGFP tag at OM14 C terminus. Uses antibiotic resistance marker natMX6.DepositorAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
SEC7-2EGFP-ter-NAT
Plasmid#184764PurposeMarker for yeast late Golgi/early endosome . Integration of 2xGFP tag at SEC7 C terminus. Uses antibiotic resistance marker natMX6.DepositorAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
CIT1-2EGFP-ter-URA3
Plasmid#184767PurposeMarker for yeast mitochondria. Integration of 2xGFP tag at CIT1 C terminus. Uses auxotrophic marker URA3(Candida albicans).DepositorAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
ACH1-2EGFP-ter-URA3
Plasmid#184769PurposeMarker for yeast mitochondria. Integration of 2xGFP tag at ACH1 C terminus. Uses auxotrophic marker URA3(Candida albicans).DepositorAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
AFG3-2EGFP-ter-URA3
Plasmid#184770PurposeMarker for yeast mitochondria. Integration of 2xGFP tag at AFG3 C terminus. Uses auxotrophic marker URA3(Candida albicans).DepositorAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
SYS1-2EGFP-ter-URA3
Plasmid#184771PurposeMarker for yeast early Golgi. Integration of 2xGFP tag at SYS1 C terminus. Uses auxotrophic marker URA3(Candida albicans).DepositorAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
P2061
Plasmid#186299PurposeExpresses LexA-HBD-B112 under the control of pAct1 and Cas9 under the control of LexA-HBD-B112+Beta-estradiol inducible promoterDepositorInsertsLexA-HBD-B112
Cas9
UseCRISPR and Synthetic BiologyExpressionBacterial and YeastPromoter2xLexop-minimalCyc1 and PACT1Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT IAA17
Plasmid#193319PurposeC-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron). ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration.DepositorInsertIAA17
TagsIAA17ExpressionYeastAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT Compatible pAIDA2(6FLAG)
Plasmid#193321PurposeC-SWAT compatible vector for C-terminal tagging with TAP-AID*-6FLAG. osTIR1 is carried downstream of the C-terminal tag.DepositorInsertTAP-AID*-6FLAG
TagsTAP-AID*-6FLAGExpressionYeastAvailable sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT AID*-6MYC osTIR1
Plasmid#193320PurposeC-SWAT compatible vector for C-terminal tagging with AID*-6MYC for auxin induced gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration into the genome.DepositorInsertAID*
Tags6MYCExpressionYeastAvailable sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only