8,128 results

1. pCSJ982

   Plasmid

   #176622

   Purpose

   Express yeast Ub-MPGLW-Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

   Depositor

   Alexander Varshavsky

   Article

   Dong et al Proc Natl Acad Sci U S A. 2020 Jun 23;11

   Insert

   Ub-MPGLW-Fbp1
   

   Tags

   Ubiquitin N-terminal fusion and triple flag tag C…

   Expression

   Yeast

   Mutation

   Changed first 4 N-terminal amino acid residues of…

   Promoter

   TDH3tc3

   Available Since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. RP51A-No5'ss-NoBS-NoPseudoBS

   Plasmid

   #100987

   Purpose

   RP51A with T7 promoter in HindIII/EcoRI sites of pUC18. No 5'ss, No BS, No pseudo BS

   Depositor

   Aaron Hoskins

   Article

   Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

   Insert

   RP51A (RPS17A Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   5'ss mut to C/ATACCT BS mut to GTTAGTG, pseu…

   Available Since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Rp51a Hyperstabilized 5'SS

   Plasmid

   #100988

   Purpose

   pUC18 Hyperstabilized Rp51a 5'SS between HindIII and EcoRI; ATG/GTATGT -> AAG/GTAAGT

   Depositor

   Aaron Hoskins

   Article

   Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

   Insert

   RP51A (RPS17A Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   5'ss mut ATG/GTATGT -> AAG/GTAAGT

   Available Since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Rp51a_10bp 5'SS_No BP or pBP_pUC18

   Plasmid

   #100989

   Purpose

   Rp51a with a tGGTAtGTta->AGGTAAGTAT 5'SS mutant with potential to form 10bps with the U1 snRNA. Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutation

   Depositor

   Aaron Hoskins

   Article

   Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

   Insert

   RP51A (RPS17A Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   tGGTAtGTta->AGGTAAGTAT 5'SS mutant, TACTA…

   Available Since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Rp51a_SUS1 5'ss_No_BP_pBP-pUC18

   Plasmid

   #100990

   Purpose

   Contains model Rp51a with a GGTAtGT->tGTAtGa 5'SS mutant (SUS1 5'SS sequence). Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutation

   Depositor

   Aaron Hoskins

   Article

   Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

   Insert

   RP51A (RPS17A Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   GGTAtGT->tGTAtGa 5'SS mutant, TACTAAC->…

   Available Since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Rp51a_SUS1 5'SS

   Plasmid

   #100991

   Purpose

   Contains model Rp51a with a GGTAtGT->tGTAtGa 5'SS mutant (SUS1 5'SS sequence).

   Depositor

   Aaron Hoskins

   Article

   Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

   Insert

   RP51A (RPS17A Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   GGTAtGT->tGTAtGa 5'SS mutant

   Available Since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Rp51a_No_BP-pUC18

   Plasmid

   #100992

   Purpose

   Contains model Rp51a with a TACTAAC->GTTAGTG BP mutataion

   Depositor

   Aaron Hoskins

   Article

   Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

   Insert

   RP51A (RPS17A Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   TACTAAC->GTTAGTG BP mutataion

   Available Since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. RP51A-NoBS-NoPseudoBS

   Plasmid

   #100986

   Purpose

   RP51A w/ T7 promoter in HindIII/EcoRI sites of pUC18. Has WT 5'ss but mutant BS and no pseudo BS

   Depositor

   Aaron Hoskins

   Article

   Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

   Insert

   RP51A (RPS17A Budding Yeast)
   

   Expression

   Bacterial and Yeast

   Mutation

   BS mutated to GTTAGTG, pseudo BS mutated to GTTTG…

   Available Since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSSa31

   Plasmid

   #179504

   Purpose

   Tet-on genome insertion system

   Depositor

   Dominique Sanglard

   Article

   Schrevens et al FEMS Yeast Res. 2022 Sep 24;22(1):foac04

   Type

   Empty backbone

   Expression

   Yeast

   Promoter

   Tet-HOP1 (p98CGH)

   Available Since

   Feb. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSSa32

    Plasmid

    #179505

    Purpose

    Tet-on genome insertion system

    Depositor

    Dominique Sanglard

    Article

    Schrevens et al FEMS Yeast Res. 2022 Sep 24;22(1):foac04

    Type

    Empty backbone

    Expression

    Yeast

    Promoter

    Tet-HOP1 (p99CGH)

    Available Since

    Feb. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFBP-2_6m.mutant

    Plasmid

    #162842

    Purpose

    Yeast expression of the mutant #2_6m for the RNA-based sensor of fructose-1,6-bisphosphate #2_6

    Depositor

    Matthias Heinemann

    Article

    Ortega et al Cell Chem Biol. 2021 Apr 22. pii: S2451-

    Insert

    2_6m_RNA-device
    

    Expression

    Yeast

    Available Since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pML104-natR412

    Plasmid

    #162042

    Purpose

    Expresses Cas9 and contains natR412 guide which targets the natR gene

    Depositor

    Jolanda Van Leeuwen

    Article

    Parts et al Mol Syst Biol. 2021 May;17(5):e10138. do

    Insert

    natR412 guide
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pML107-natR854

    Plasmid

    #162043

    Purpose

    Expresses Cas9 and contains natR854 guide which targets the natR gene

    Depositor

    Jolanda Van Leeuwen

    Article

    Parts et al Mol Syst Biol. 2021 May;17(5):e10138. do

    Insert

    natR854 guide
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDN513

    Plasmid

    #175365

    Purpose

    Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. Low copy, HIS3-selected.

    Depositor

    Daniel Nickerson

    Article

    Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDN514

    Plasmid

    #175366

    Purpose

    Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. Low copy, TRP1-selected.

    Depositor

    Daniel Nickerson

    Article

    Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDN515

    Plasmid

    #175367

    Purpose

    Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. Low copy, LEU2-selected.

    Depositor

    Daniel Nickerson

    Article

    Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDN516

    Plasmid

    #175368

    Purpose

    Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. Low copy, URA3-selected.

    Depositor

    Daniel Nickerson

    Article

    Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDN523

    Plasmid

    #175369

    Purpose

    Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, HIS3-selected.

    Depositor

    Daniel Nickerson

    Article

    Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDN524

    Plasmid

    #175370

    Purpose

    Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, TRP1-selected.

    Depositor

    Daniel Nickerson

    Article

    Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDN525

    Plasmid

    #175371

    Purpose

    Yeast shuttle plasmid that allows conversion between low copy, high copy, and integrating vectors. High copy, LEU2-selected.

    Depositor

    Daniel Nickerson

    Article

    Nickerson et al G3 (Bethesda). 2021 Dec 8;11(12). pii: 6

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge