8,173 results

1. p2B-56

   Plasmid

   #177925

   Purpose

   Expresses optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter

   Depositor

   Vadim Gladyshev

   Article

   Barre et al (unpublished)

   Insert

   Optimized unleaky reverse tetracycline transactivator (rtTA-M2-SE-G72P) under control of yeast MYO2 promoter
   

   Expression

   Bacterial and Yeast

   Mutation

   V9I; F67S; G72P, F86Y; R171K

   Promoter

   MYO2

   Available Since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFA6a-link-yoTagRFP-T-Kan

   Plasmid

   #44906

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized TagRFP-T with Kan selection

   Depositor

   Wendell Lim, Kurt Thorn

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoTagRFP-T
   

   Expression

   Yeast

   Available Since

   Oct. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFA6a-6xGLY-V5-kanMX6

   Plasmid

   #20780

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Expression

   Yeast

   Available Since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYX233-DHFR

   Plasmid

   #163761

   Purpose

   Gal-inducible yeast expression of cytosolic DHFR

   Depositor

   Johannes Herrmann

   Article

   Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

   Insert

   DHFR (Dhfr Mouse)
   

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST-DHFR F[3]-C (LEU2)

   Plasmid

   #177796

   Purpose

   Gateway destination vector to insert genes of interest having a C-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.

   Depositor

   Christian Landry

   Article

   Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

   Type

   Empty backbone

   Tags

   DHFR F[3]

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   Jan. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDEST-DHFR F[1,2]-C (TRP1)

   Plasmid

   #177795

   Purpose

   Gateway destination vector to insert genes of interest having a C-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

   Depositor

   Christian Landry

   Article

   Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

   Type

   Empty backbone

   Tags

   DHFR F[1,2]

   Expression

   Yeast

   Promoter

   ADH1

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRS42H_PTEF1_dCas9_TCYC1

   Plasmid

   #177155

   Purpose

   Expresses dCas9 in yeast cells

   Depositor

   Gautam Dantas

   Article

   Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

   Insert

   Nuclease-null Cas9
   

   Tags

   SV40 NLS

   Expression

   Yeast

   Promoter

   TEF1 promoter

   Available Since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJH2971

   Plasmid

   #100955

   Purpose

   Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. KANMX marker

   Depositor

   James Haber

   Article

   Ranjith et al Protocol Exchange (2017)

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   Sept. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. ZJOM143

   Plasmid

   #133667

   Purpose

   Integration of VPH1-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast vacuole.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-VPH1-mTagBFP2 (VPH1 Budding Yeast)
   

   Tags

   mTagBFP2

   Expression

   Yeast

   Available Since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSH100 (YCplac33 MET25pro MCP-mCherry)

    Plasmid

    #45930

    Depositor

    Robert Singer, Daniel Zenklusen

    Article

    Hocine et al Nat Methods. 2013 Feb;10(2):119-21. doi:

    Insert

    MCP-mCherry
    

    Tags

    mCherry

    Expression

    Yeast

    Promoter

    Met25

    Available Since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pG14-MS2-GFP

    Plasmid

    #27117

    Depositor

    Robert Singer

    Article

    Bertrand et al Mol Cell. 1998 Oct . 2(4):437-45.

    Insert

    MS2-binding protein
    

    Tags

    GFP, HA, and NLS

    Expression

    Yeast

    Available Since

    Dec. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPS2040

    Plasmid

    #1372

    Depositor

    Pamela Silver

    Article

    Brodsky et al RNA 2000 Dec;6(12):1737-49.

    Insert

    PGK1-U1A-ASH1 3' UTR (PGK1 Budding Yeast)
    

    Expression

    Yeast

    Available Since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

13. pXN21-Dest

    Plasmid

    #105086

    Purpose

    Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

    Depositor

    Christopher Grefen

    Article

    Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

    Type

    Empty backbone

    Tags

    NubG

    Expression

    Yeast

    Available Since

    Feb. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pML104-HygMx4

    Plasmid

    #83475

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.

    Depositor

    Patricia Wittkopp

    Insert

    HphMX4
    

    Expression

    Yeast

    Available Since

    Oct. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pL-RPB1

    Plasmid

    #10981

    Depositor

    Jeff Strathern

    Article

    Malagon et al Genetics. 2006 Apr . 172(4):2201-9.

    Insert

    RPB1 (RPO21 Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Mutation

    Wild type RPB1 gene

    Available Since

    Dec. 16, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG413GPD-ccdB

    Plasmid

    #14142

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Expression

    Yeast

    Available Since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETCON-PTX-S1-220

    Plasmid

    #67747

    Purpose

    Pertussis Toxin Subunit 1 Yeast Display Plasmid

    Depositor

    Timothy Whitehead

    Article

    Kowalsky et al J Biol Chem. 2015 Oct 30;290(44):26457-7

    Insert

    PTxS1-220
    

    Expression

    Bacterial and Yeast

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pABY-T2

    Plasmid

    #201765

    Purpose

    For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

    Depositor

    Saravanan Palani

    Article

    Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

    Insert

    ALFA tag
    

    Expression

    Yeast

    Available Since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pG3

    Plasmid

    #162604

    Purpose

    Edit Ade2 gene in yeast

    Depositor

    Lars Steinmetz

    Article

    Sankaran et al J Microbiol Biol Educ. 2021 May 31;22(2)

    Insert

    Tef1-Cas9 with RPL25 Intron
    

    Expression

    Yeast

    Available Since

    June 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pABY-c15

    Plasmid

    #201768

    Purpose

    Expresses mNeonGreen-tagged NbALFA under ADH1 promoter in yeast

    Depositor

    Saravanan Palani

    Article

    Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

    Insert

    NbALFA
    

    Tags

    40aa Linker, mNeonGreen

    Expression

    Yeast

    Promoter

    ADH1

    Available Since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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