8,418 results

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CPH3569 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,K1487N,D1497K; msLink2)

Plasmid

#91820

Purpose

Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD

Depositor

Chris Hill

Publication

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Tags

10xHis

Expression

Yeast

Mutation

5xGly linker, PreScission Protease site (LEVLFQGP…

Promoter

Endogenous Rpb1 promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3400 (YCp LEU2 Rpb1 A1529_G1534delInsLEVLFQGP)

Plasmid

#91806

Purpose

Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site

Depositor

Chris Hill

Publication

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

Residues A1529-G1534 replaced with a PreScission …

Promoter

Endogenous Rpb1 promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3477 (YCp LEU2 Rpb1 P1455_E1456InsLEVLFQGP)

Plasmid

#91807

Purpose

Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site

Depositor

Chris Hill

Publication

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

PreScission Protease site (LEVLFQGP) inserted bet…

Promoter

Endogenous Rpb1 promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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CPH3486 (YCp LEU2 Rpb1 F1492A)

Plasmid

#91808

Purpose

Yeast expression of S. cerevisiae Rpb1 with a F1492A mutation

Depositor

Chris Hill

Publication

Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

Insert

RPO21 (RPO21 Budding Yeast)

Expression

Yeast

Mutation

changed phenylalanine 1492 to alanine

Promoter

Endogenous Rpb1 promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pVG50

Plasmid

#164708

Purpose

yEVenus under the control of tetOpr with ADH1t upstream of the reporter

Depositor

Sahand Jamal Rahi

Publication

Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

Insert

ADH1t - tetOpr - yEVenus - CLN2 PEST - ADH1t

Expression

Yeast

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pVG88

Plasmid

#164709

Purpose

yEVenus under the control of ARG3 promoter

Depositor

Sahand Jamal Rahi

Publication

Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

Insert

ARG3pr - yEVenus - CLN2 PEST - ADH1t

Expression

Yeast

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-KAR2-mEGFP-HDEL

Plasmid

#160467

Purpose

Yeast integration vector for the gene replacement of S. cerevisiae KAR2 fused to mEGFP with an ER retrieval signal

Depositor

Benjamin Glick

Publication

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

KAR2-mEGFP-HDEL

Expression

Yeast

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCL10

Plasmid

#164710

Purpose

yEVenus under the control of MET3 promoter

Depositor

Fred Cross

Publication

Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

Insert

MET3pr - yEVenus - CLN2 PEST - ADH1t

Expression

Yeast

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19-PpSEC13-mEGFP

Plasmid

#160469

Purpose

P. pastoris integration vector for the gene replacement of SEC13 fused to mEGFP

Depositor

Benjamin Glick

Publication

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

ppSEC13-mEGFP

Expression

Yeast

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19-PpSEC13-msGFP2

Plasmid

#160470

Purpose

P. pastoris integration vector for the gene replacement of SEC13 fused to msGFP2

Depositor

Benjamin Glick

Publication

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

ppSEC13-msGFP2

Expression

Yeast

Available since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2 ΔN

Plasmid

#163575

Purpose

express wild type Dcp2 ΔN(243-970) at endogenous level in S.cerevisiae

Depositor

Roy Parker

Publication

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

Truncation of N-terminal domain (1-242) of Dcp2

Promoter

DCP2 promoter

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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Dcp2 300

Plasmid

#163577

Purpose

express Dcp2 300 at endogenous level in S.cerevisiae

Depositor

Roy Parker

Publication

Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1

Insert

Dcp2 (DCP2 Budding Yeast)

Tags

GFP

Expression

Yeast

Mutation

Truncation of C-terminal domian (301-970)

Promoter

DCP2 promoter

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI751 pPGK1-GCN4(UTR)-ZEM(TF)

Plasmid

#164916

Purpose

Expresses the ZEM transcription factor under the translational control of the yeast GCN4 5'UTR

Depositor

Nicholas Ingolia

Publication

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

GCN4(UTR)-ZEM(TF)

Use

Synthetic Biology

Expression

Yeast

Promoter

PGK1 (yeast)

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI743 dual-barcoded gRNA parent vector

Plasmid

#164915

Purpose

CEN/ARS Parent vector for constructing barcoded-gRNA CiBER-seq plasmid libraries

Depositor

Nicholas Ingolia

Publication

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI733 pRPR1(TetO)-RPC31-sgRNA

Plasmid

#164913

Purpose

For yeast genomic integration of sgRNA against RPC31

Depositor

Nicholas Ingolia

Publication

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

pRPR1(TetO)-RPC31-sgRNA

Use

CRISPR

Expression

Yeast

Promoter

RPR1

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI732 pRPR1(TetO)-HTS1-sgRNA

Plasmid

#164912

Purpose

For yeast genomic integration of sgRNA against HTS1

Depositor

Nicholas Ingolia

Publication

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

pRPR1(TetO)-HTS1-sgRNA

Use

CRISPR

Expression

Yeast

Promoter

RPR1

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pPEE42

Plasmid

#139421

Purpose

Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the Citrine ORF and a flexible linker at the 5' end.

Depositor

James Fraser

Publication

Spencer et al Fungal Genet Biol. 2020 May;138:103365.

Insert

Citrine

Expression

Yeast

Promoter

TEF1

Available since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pRS423-ProSgH

Plasmid

#163974

Purpose

Helper plasmid for cloning gRNAs under the control of the tRNA(Pro) promoter

Depositor

Jiazhang Lian

Publication

Dong et al ACS Synth Biol. 2020 Sep 18;9(9):2252-22

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pPAP003

Plasmid

#153490

Purpose

Level 2 integrative plasmid for stable expression in P. pastoris

Depositor

Sylvestre Marillonnet

Publication

Pullmann et al Commun Biol. 2021 May 12;4(1):562. doi:

Type

Empty backbone

Expression

Yeast

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pWL89A mmPing20F

Plasmid

#145789

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock

Publication

Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

Insert

mmPing20F

Expression

Yeast

Mutation

Insertion of the FMV enhancer

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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