8,133 results

1. PP300

   Plasmid

   #78940

   Purpose

   Expresses GFP. Figure 3. Strain 7.

   Depositor

   Timothy Lu

   Article

   Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

   Insert

   GFP
   

   Expression

   Yeast

   Available Since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PP228

   Plasmid

   #78942

   Purpose

   Expresses GFP. Figures 3 and 4. Strain 8. Promoter: ppTEF1, minimal Promoter: mCYC.

   Depositor

   Timothy Lu

   Article

   Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

   Insert

   GFP
   

   Expression

   Yeast

   Available Since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PP77

   Plasmid

   #78947

   Purpose

   Supplemental Figure 3. Integration Site 4.

   Depositor

   Timothy Lu

   Article

   Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

   Insert

   Integration Site 4
   

   Expression

   Yeast

   Available Since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PP79

   Plasmid

   #78949

   Purpose

   Supplemental Figure 3. Integration Site 6.

   Depositor

   Timothy Lu

   Article

   Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

   Insert

   Integration Site 6
   

   Expression

   Yeast

   Available Since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PP69

   Plasmid

   #78950

   Purpose

   Supplemental Figure 3. Integration Site 7.

   Depositor

   Timothy Lu

   Article

   Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

   Insert

   Integration Site 7
   

   Expression

   Yeast

   Available Since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. PP295

   Plasmid

   #78935

   Purpose

   Expresses GFP. Figure 3. Strain 1.

   Depositor

   Timothy Lu

   Article

   Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

   Insert

   GFP
   

   Expression

   Yeast

   Available Since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PP296

   Plasmid

   #78936

   Purpose

   Expresses GFP. Figure 3. Strain 2.

   Depositor

   Timothy Lu

   Article

   Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

   Insert

   GFP
   

   Expression

   Yeast

   Available Since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pWHI2-HYG

   Plasmid

   #80589

   Purpose

   CEN plasmid expressing full-length WHI2 from S.cerevisiae S288C background under its native promoter

   Depositor

   John McCusker

   Article

   Vijayraghavan et al Genetics. 2018 Nov 29. pii: genetics.118

   Insert

   WHI2 (WHI2 Budding Yeast)
   

   Expression

   Yeast

   Promoter

   WHI2 native promoter

   Available Since

   July 28, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pFA6a-pcr1P-natMX6-pcr2P

   Plasmid

   #80620

   Purpose

   to delete part of the mat1-P locus and fix the mating type in natural S. pombe isolates

   Depositor

   Miguel Godinho Ferreira

   Article

   Avelar et al Nat Commun. 2013;4:2235. doi: 10.1038/nc

   Inserts

   PCR1 from mat1-P
   PCR2 from mat1-P
   

   Expression

   Yeast

   Available Since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFA6a-pcr1M-natMX6-pcr2M

    Plasmid

    #80621

    Purpose

    to delete part of the mat1-M locus and fix the mating type in natural S. pombe isolates

    Depositor

    Miguel Godinho Ferreira

    Article

    Avelar et al Nat Commun. 2013;4:2235. doi: 10.1038/nc

    Inserts

    PCR1 from mat1-M
    PCR2 from mat1-M
    

    Expression

    Yeast

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pREP81xV5_ccdB

    Plasmid

    #79019

    Purpose

    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, LEU2 selction

    Depositor

    Göran Akusjärvi, Pernilla Bjerling

    Type

    Empty backbone

    Tags

    double V5 epitope tag

    Expression

    Yeast

    Available Since

    July 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pREP41xV5_ccdB

    Plasmid

    #79017

    Purpose

    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, LEU2 selection

    Depositor

    Göran Akusjärvi, Pernilla Bjerling

    Type

    Empty backbone

    Tags

    double V5 epitope tag

    Expression

    Yeast

    Available Since

    July 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pREP42xN_ccdB

    Plasmid

    #79024

    Purpose

    Gateway destination vector for S. pombe. Allow N-terminal tag(s) to be introduced using MCS. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, ura4+ selection

    Depositor

    Göran Akusjärvi, Pernilla Bjerling

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    July 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pREP82xV5_ccdB

    Plasmid

    #79020

    Purpose

    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag.attR1-CmR-ccdB-attR2 cassette, nmt1** (AT) promoter, ura4+ selection

    Depositor

    Göran Akusjärvi, Pernilla Bjerling

    Type

    Empty backbone

    Tags

    double V5 epitope tag

    Expression

    Yeast

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pREP42xV5_ccdB

    Plasmid

    #79018

    Purpose

    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1* (ATAAA) promoter, ura4+ selection

    Depositor

    Göran Akusjärvi, Pernilla Bjerling

    Type

    Empty backbone

    Tags

    double V5 epitope tag

    Expression

    Yeast

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDA186

    Plasmid

    #74250

    Purpose

    Intermediate cloning vector, with MCS2 containing inducible unstable peptide driven by pGPD1 (UbiY 2xNLSs SZ3).

    Depositor

    Serge Pelet

    Article

    Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10

    Insert

    Inducible peptide of the dPSTR
    

    Expression

    Bacterial and Yeast

    Promoter

    pGPD1

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDA171

    Plasmid

    #74251

    Purpose

    Intermediate cloning vector, with MCS2 containing inducible stable peptide driven by pSTL1 (Venus 2xNLSs SZ1).

    Depositor

    Serge Pelet

    Article

    Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10

    Insert

    Inducible peptide of the dPSTR
    

    Tags

    Venus

    Expression

    Bacterial and Yeast

    Promoter

    pSTL1

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDA199

    Plasmid

    #74275

    Purpose

    pSTL1-dPSTRy(4,3)

    Depositor

    Serge Pelet

    Article

    Aymoz et al Nat Commun. 2016 Apr 21;7:11304. doi: 10

    Insert

    pSTL1-dPSTRy(4,3)
    

    Expression

    Bacterial and Yeast

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pNIA-CEN-FLAG-LINXa4-Bre1

    Plasmid

    #73617

    Purpose

    Control of the E3 ligase Bre1 with LINXa in yeast.

    Depositor

    Brian Kuhlman

    Article

    Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

    Insert

    LINXa4-Bre1dNLS
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Bre1 point mutations K8A, K9A and K11A

    Promoter

    ADH1

    Available Since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJK139

    Plasmid

    #73315

    Purpose

    Pas2p peroxisomal membrane biogenesis protein

    Depositor

    T. J. Kappock

    Insert

    Pas2p peroxisomal membrane targeting protein
    

    Tags

    His6 and c-myc

    Expression

    Yeast

    Mutation

    Encodes a glycine-204 to serine mutation

    Promoter

    AOX1

    Available Since

    March 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge