8,057 results

1. pFA6a-mYFP-hphMX6

   Plasmid

   #105155

   Purpose

   Yeast gene targeting

   Depositor

   Thomas Pollard, Jian-Qiu Wu

   Article

   Wu et al Dev Cell. 2003 Nov;5(5):723-34.

   Insert

   mYFP-hphMX6
   

   Use

   Tags

   mYFP

   Expression

   Bacterial and Yeast

   Mutation

   A206K

   Promoter

   Available since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. BS-Ura3-GFP-Atg8

   Plasmid

   #69194

   Purpose

   Single copy integration of GFP-Atg8, use auxotrophic marker URA3(Kluyveromyces lactis)

   Depositor

   Zhiping Xie

   Article

   Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

   Insert

   GFP-Atg8
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFA6a-link-yoSuperfolderGFP-SpHis5

   Plasmid

   #44837

   Purpose

   Yeast tagging vector to create gene fusion with yeast optimized SuperfolderGFP with SpHis5 selection

   Depositor

   Kurt Thorn, Wendell Lim

   Article

   Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

   Insert

   yoSuperfolderGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCfB2223

   Plasmid

   #67544

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae X-3 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWS3865

   Plasmid

   #185742

   Purpose

   Sub-array backbone 1/4

   Depositor

   Rodrigo Ledesma-Amaro

   Article

   Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRS402-GPD-GFP(S65T)

   Plasmid

   #85190

   Purpose

   Integrating plasmid, GFP(S65T) under GPD promoter

   Depositor

   David Bartel

   Article

   Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

   Insert

   EGFP
   

   Use

   Integrating, shuttle vector

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GPD

   Available since

   Dec. 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBBK21 pCAS-Tyr-[gRNA: 4=XII-5] (SplitHygR, AmpR)

   Plasmid

   #179005

   Purpose

   Sp.Cas9 and gRNA yeast expression vector withXII-5 gRNA pre-cloned. Selection =SplitHygromycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBBK20 pCAS-Tyr-[gRNA: 3=XII-2] (SplitHygR, AmpR)

   Plasmid

   #179004

   Purpose

   Sp.Cas9 and gRNA yeast expression vector with XII-2 gRNA pre-cloned. Selection =SplitHygromycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFA6a-link-mCherry-SpHis5

   Plasmid

   #44643

   Purpose

   Yeast tagging vector to create gene fusion with mCherry with SpHis5 selection

   Depositor

   Kurt Thorn

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFA6a-hphMX-(3×FLAG)-TEV-ProtA

    Plasmid

    #52692

    Purpose

    For C-terminal tagging of ORFs with a (3×FLAG)-TEV-ProtA

    Depositor

    Michael Nick Boddy

    Insert

    (3×FLAG)-TEV-ProtA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    April 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDRF1 mScarlet3-T2A-mTurquoise2

    Plasmid

    #219848

    Purpose

    equimolar expression of mScarlet3 and mTurquoise2 in budding yeast

    Depositor

    Bas Teusink

    Article

    Botman et al bioRxiv 2025.06.27.661975; doi: https://

    Insert

    mScarlet3-T2A-mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. PCUP1-DuDre-Atg8-404

    Plasmid

    #69201

    Purpose

    RFP-Atg8 (PAS marker) with improved property

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    DuDre-Atg8
    

    Use

    Tags

    DsRed Express 2

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDRF1-GW ytdTomato

    Plasmid

    #118456

    Purpose

    Overexpression of yeast codon-optimized tdTomato

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ytdTomato
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDRf1-GW

    Plasmid

    #36026

    Depositor

    Dominique Loque, Wolf Frommer

    Article

    Loque et al Nature. 2007 Mar 8;446(7132):195-8. Epub

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFA6a-his3-P41nmt1-3FLAG

    Plasmid

    #19340

    Depositor

    Eishi Noguchi

    Article

    Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

    Insert

    his3 (his3 Fission Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAG426-GAL-Alpha-synuclein-E46K

    Plasmid

    #181723

    Purpose

    Galactose inducible expression of Alpha-synuclein-E46K

    Depositor

    Alejandro Chavez

    Insert

    Alpha-synuclein (SNCA Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    E46K

    Promoter

    Available since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFA6a-6xGLY-His-tag-HIS3MX6

    Plasmid

    #20762

    Depositor

    Mark Hochstrasser

    Article

    Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

    Type

    Empty backbone

    Use

    Pcr-based c-terminal tagging

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFA6a-mNeonGreen-HIS3MX6

    Plasmid

    #129100

    Purpose

    For tagging genes at 3'end (C-terminal end of protein) in S. cerevisiae with mNeonGreen

    Depositor

    Wei-Lih Lee

    Insert

    mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFA6a-mScarlet-I-mNeonGreen-kanMX (pKBJ007)

    Plasmid

    #173454

    Purpose

    Contains mScarletI-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mScarlet-I-mNeonGreen
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    no promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCfB2311 (SNR52p-gRNA.ADE2-SUP4t_natMX)

    Plasmid

    #83947

    Purpose

    gRNA cassette-carrying vector with natMX marker

    Depositor

    Irina Borodina

    Article

    Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

    Insert

    gRNA targeting ADE2 gene
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge