8,418 results
-
Plasmid#8820DepositorInsertbeta-gal
TagsUbiquitinExpressionYeastMutationL (leucine) at amino terminus of beta-galAvailable sinceDec. 28, 2005AvailabilityAcademic Institutions and Nonprofits only -
pL-rpb1-N488D
Plasmid#10988DepositorInsertrpb1-N488D (RPO21 Budding Yeast)
ExpressionBacterial and YeastMutationMutation 1462 A to G in the RPB1 ORF resulting in…Available sinceDec. 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-TRP1
Plasmid#11007DepositorInsertTRP1 (TRP1 Budding Yeast)
TagsV5 and his6ExpressionBacterial and YeastMutationORF-TRP1 without stop codonAvailable sinceDec. 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
-
pPS1372
Plasmid#1353DepositorInsertpADH-NLS-NES-GFP2
ExpressionYeastAvailable sinceAug. 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
pYAL_HLA-A2_MART1
Plasmid#171177PurposeDisplays MART-1 peptide in HLA-A*02 as a single-chain trimerDepositorInsertMART1_HLA-A2*01 single-chain trimer fusion
ExpressionBacterial and YeastPromoterGAL1Available sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
-
-
-
pGR596
Plasmid#213787PurposeIntegrates at the CAN1 locus the BadBoy3 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassetteDepositorInsertBadBoy3 TP-DNAP1
UseSynthetic BiologyExpressionBacterial and YeastMutationSee manuscriptAvailable sinceMarch 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pABY-c45
Plasmid#201771PurposeExpresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeastDepositorInsertNbALFA
Tags40aa Linker, mNeonGreenExpressionYeastPromoterTEF1Available sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSC5
Plasmid#188602PurposeSuperior Conjugative Plasmid to Diverse YeastDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-ATG8(416)/GFP-AUT7(416)
Plasmid#160389PurposeExpresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter , NAT selectionDepositorInsertautophagy-related 8 (ATG8 Budding Yeast)
UseCen/arsExpressionYeastPromoterEndogenous ATG8 promoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPT24-mtnluc
Plasmid#184339PurposeTransformation of mitochondrial DNA in yeastDepositorInsertnanoluciferase recoded for mitochondrial expression flanked by COX2 UTRs
ExpressionYeastAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
pYM28-BirA*
Plasmid#160290PurposeUsed for C-terminal BirA* tagging of proteins in Saccharomyces cerevisiae; Selection marker: HIS3DepositorInsertBirA*
ExpressionYeastAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ZJOM43
Plasmid#133652PurposeIntegration of VPS4-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.DepositorAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046483
Plasmid#153868PurposeYeast expression plasmid for SARS-CoV-2 nsp3DepositorInsertSARS-CoV-2 nsp3 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
TagsCleavable thrombin;6xHISExpressionYeastMutationwtPromoterpGAL1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBKWH-p53
Plasmid#133900PurposePositive control when used in combination with pAWH-largeT. Expression of Gal4BD-p53 hybrid protein. Homology regions for recombination with pAWHDepositorAvailable sinceJune 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM10
Plasmid#133639PurposeIntegration of VPS4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast late endosome.DepositorAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only