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pYC54

Plasmid

#63909

Purpose

Recipient integrative vector to construct YFP tagged fusions

Depositor

Irene Castano Navarro

Publication

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Tags

YFP

Expression

Yeast

Available since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pNB648

Plasmid

#60140

Purpose

NanoLuc luciferase with a nuclear localization signal (NLS) driven by MET17 promoter.

Depositor

Nicolas Buchler

Publication

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

NLuc

Tags

Nuclear localization signal (NLS)

Expression

Yeast

Promoter

MET17pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pNB778

Plasmid

#60154

Purpose

Green click beetle luciferase (Promega) driven by LEU1 promoter.

Depositor

Nicolas Buchler

Publication

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

CBG99

Expression

Yeast

Promoter

LEU1pr

Available since

Nov. 13, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRS416-PHO5-GFP-Osh1

Plasmid

#58835

Purpose

Expresses GFP-Tagged Osh1 in yeast

Depositor

Tim Levine

Publication

Loewen et al EMBO J. 2003 May 1;22(9):2025-35.

Insert

Osh1 (SWH1, OSH1 Budding Yeast)

Tags

GFP

Expression

Yeast

Promoter

PHO5

Available since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only
pRS406-PHO5-GFP-2xOsh2 PH domain [short]

Plasmid

#58829

Purpose

Expresses GFP-Tagged Osh2 PH domain in yeast

Depositor

Tim Levine

Publication

Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

Insert

2xOsh2 PH domain [short] (OSH2 Budding Yeast)

Tags

GFP and Myc

Expression

Yeast

Promoter

PHO5

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
D771 leu2::LYS2 Disruptor Converter

Plasmid

#51677

Purpose

Yeast marker swap plasmid for converting LEU2 to LYS2

Depositor

David Stillman

Publication

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

LYS2 (LYS2 Budding Yeast)

Use

Yeast marker swap

Expression

Yeast

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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p413TEF-Sir2

Plasmid

#51742

Purpose

overexpression of Sir2 in S. cerevisiae, HIS3 marker

Depositor

Markus Ralser

Publication

Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

Insert

Sir2 (SIR2 Budding Yeast)

Expression

Yeast

Promoter

TEF1

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-12Pk-ura4MX6

Plasmid

#49180

Purpose

genomic 12Pk epitope tagging in S. pombe

Depositor

Eishi Noguchi

Publication

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Tags

12Pk

Expression

Yeast

Available since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only

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PRS415-Gal1pr-Mag1-sctetR

Plasmid

#44752

Depositor

Narendra Maheshri

Publication

Finney-Manchester et al Nucleic Acids Res. 2013 Mar 7.

Insert

MAG1-sctetR

Expression

Yeast

Promoter

Gal1pr

Available since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pXP741

Plasmid

#46059

Depositor

Nancy DaSilva

Publication

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Expression

Yeast

Promoter

ADH2

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pXP841

Plasmid

#46067

Depositor

Nancy DaSilva

Publication

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Expression

Yeast

Promoter

ADH2

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yECitrine-Hygro

Plasmid

#44646

Depositor

Kurt Thorn

Insert

yECitrine

Expression

Yeast

Available since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yECFP-Hygro

Plasmid

#44647

Depositor

Kurt Thorn

Insert

yEmCFP

Expression

Yeast

Available since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yEPAGFP-CaUra3

Plasmid

#44649

Depositor

Kurt Thorn

Insert

yEPAGFP

Expression

Yeast

Available since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

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pDN-T2dMFot

Plasmid

#44558

Depositor

Gabor Balazsi

Publication

Nevozhay et al PLoS Comput Biol. 2012;8(4):e1002480. do

Inserts

PTETREG promoter
rtetR-M2::FFF

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Compared to rtTA, rtTA-M2 has S12G, E19G, A56P, D…

Promoter

PTETREG

Available since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pDN-G1Gh

Plasmid

#44507

Depositor

Gabor Balazsi

Publication

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
yEGFP

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Two tandem tet operators (2XtetO2) downstream of …

Promoter

PGal1-D12 promoter

Available since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pAG306-GPD-AVT1 chr I

Plasmid

#41898

Purpose

Yeast expression of AVT1.

Depositor

Dan Gottschling

Publication

Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

Insert

AVT1 (AVT1 Budding Yeast)

Expression

Yeast

Promoter

GPD

Available since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only

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pZHY500

Plasmid

#36186

Depositor

Daniel Voytas

Publication

Zhang et al Plant Physiol. 2012 Nov 2.

Insert

FokI homodimeric Nuclease domain

Tags

ACV5

Expression

Yeast

Mutation

delta152,+63 truncation to TAL backbone

Promoter

TEV

Available since

Nov. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pDS244

Plasmid

#34982

Depositor

Michael Glotzer

Publication

Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

Insert

Ste11 (STE11 Budding Yeast)

Expression

Yeast

Promoter

ADH

Available since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pTSK368

Plasmid

#34551

Depositor

James McNally

Publication

Karpova et al Science. 2008 Jan 25;319(5862):466-9.

Insert

MS2-CP-NLS

Tags

GFP

Expression

Yeast

Promoter

Met25

Available since

Jan. 19, 2012

Availability

Academic Institutions and Nonprofits only

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