8,418 results

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pDRF1 mBaojin-T2A-mCherry

Plasmid

#219842

Purpose

equimolar expression of mBaojin and mCherry in budding yeast

Depositor

Bas Teusink

Publication

Botman et al ACS Synth Biol. 2026 Apr 1. doi: 10.1021

Insert

mBaojin-T2A-mCherry

Expression

Yeast

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pABY-T2

Plasmid

#201765

Purpose

For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

Depositor

Saravanan Palani

Publication

Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

Insert

ALFA tag

Expression

Yeast

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pFA6a-yomRuby3-SpHIS5

Plasmid

#204287

Purpose

For C-terminal tagging using yeast optimized mRuby3

Depositor

Linda Huang

Publication

Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

Insert

pFA6-yomRuby3-SpHIS5

Tags

yomRuby3

Expression

Yeast

Promoter

none

Available since

July 25, 2023

Availability

Academic Institutions and Nonprofits only

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pGS_128

Plasmid

#160651

Purpose

Expresses Human APOE3 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoter

Depositor

Susan Lindquist, Li-Huei Tsai

Publication

Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

Insert

Apolipoprotein E e3 (APOE Human)

Tags

Kar2 signal sequence

Expression

Yeast

Mutation

e3 allele

Promoter

pZ estradiol inducible promoter

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pWS3865

Plasmid

#185742

Purpose

Sub-array backbone 1/4

Depositor

Rodrigo Ledesma-Amaro

Publication

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Expression

Yeast

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-HALO-NAT

Plasmid

#188931

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Publication

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-HALO-NAT

Expression

Yeast

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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p413TEF1-ATR1(pYL573)

Plasmid

#178288

Purpose

Expresses NADPH-cytochrome P450 reductase 1 from A. thaliana (ATR1) in Saccharomyces cerevisiae. Centromeric HIS, PTEF1-ATR1-TCYC1

Depositor

Yanran Li

Publication

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

ATR1

Expression

Yeast

Promoter

TEF1

Available since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pDIV537

Plasmid

#177704

Purpose

Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting KU70 homolog in D. hansenii

Depositor

Uffe Mortensen

Publication

Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

Inserts

Cas9
Nat
tRNA-sgRNA-tRNA

Use

CRISPR

Tags

SV40

Expression

Bacterial and Yeast

Promoter

RNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDEST-DHFR F[3]-N (LEU2)

Plasmid

#177798

Purpose

Gateway destination vector to insert genes of interest having a N-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.

Depositor

Christian Landry

Publication

Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

Type

Empty backbone

Expression

Yeast

Promoter

ADH1

Available since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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EC2_3_dCas9_Mxi1_sgRNA

Plasmid

#163708

Purpose

Yeast low copy plasmid with dCas9, sgRNA expression cassette and Mxi1 transcriptional repressor

Depositor

Yvonne Nygård

Publication

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Inserts

dCas9
Mxi1+SV40 NLS

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pWS176

Plasmid

#90963

Purpose

Cas9 gap repair vector - ZeoR

Depositor

Tom Ellis

Insert

Cas9

Use

CRISPR

Expression

Yeast

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLyGo-Kp-1

Plasmid

#163143

Purpose

pLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-MF pre-sequence and the LyGo cassette (SapI-ccdB-SapI)

Depositor

Morten Norholm

Publication

Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Promoter

AOX1

Available since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pXZX501

Plasmid

#169455

Purpose

pRS426-SNR52p-gRNA.MATa_option3-SUP4t; 20-bp gRNA sequence targeting MATa cassette

Depositor

Jef Boeke

Publication

Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

Insert

gRNA for MATa

Expression

Yeast

Promoter

SNR52

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pNA0306

Plasmid

#169452

Purpose

pRS415-TEF1p-Cas9-CYC1t; Cas9 expression plasmid

Depositor

Jef Boeke

Publication

Xie et al G3 (Bethesda). 2018 Jan 4;8(1):173-183.

Insert

Cas9

Expression

Yeast

Promoter

TEF1p

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCSN075

Plasmid

#166732

Purpose

Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiae

Depositor

Rene Verwaal

Publication

Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

Insert

dCas12a-NLS-Mxi1

Use

CRISPR

Tags

Mxi1 and SV40 NLS

Expression

Yeast

Mutation

E925A

Promoter

S. cerevisiae TEF1

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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yo-msGFP2

Plasmid

#160471

Purpose

Encodes a yeast codon optimized version of msGFP2

Depositor

Benjamin Glick

Publication

Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

Insert

yo-msGFP2

Expression

Yeast

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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Cip-pAct1-Mnase-SAT1

Plasmid

#162557

Purpose

For the C. auris MNase control strain, the URA3 auxotrophy marker of the CIp-pACT1-3xFLAG-MNase-SV40-CYC was replaced by SAT1

Depositor

Adnane Sellam

Publication

Tebbji et al mSphere. 2020 Oct 14;5(5). pii: 5/5/e006

Insert

3FLAG-Mnase-SV40

Expression

Yeast

Promoter

Actine

Available since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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ZJOM57

Plasmid

#133656

Purpose

Integration of PEX3-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast peroxisome.

Depositor

Zhiping Xie

Publication

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-PEX3-DuDre (PEX3 Budding Yeast)

Tags

DuDre

Expression

Yeast

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pMTU-DO-HPH

Plasmid

#160008

Purpose

Low-copy expression vector for K. marxianus, hygromycin selection

Depositor

John Morrissey

Publication

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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p416-TEF-membrane localization signal-tagBFP-wrmScarlet11-TEF terminator

Plasmid

#158585

Purpose

Expresses membrane localized tagBFP with wrmScarlet11 C-terminal fusion in S. cerevisiae

Depositor

Cynthia Kenyon

Publication

Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

Insert

membrane localization signal-tagBFP-wrmScarlet11

Tags

tagBFP

Expression

Yeast

Mutation

GGGS linker

Promoter

TEF

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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