8,086 results

1. YIplac211-Mup1-mScarlet2I

   Plasmid

   #140152

   Purpose

   Plasmid for pop-in/pop-out replacement of Mup1 with Mup1-mScarlet2I

   Depositor

   Benjamin Glick

   Article

   Casler et al Elife. 2020 Jun 25;9. pii: 56844. doi: 1

   Insert

   MUP1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRS416-PHO5-GFP-Osh1

   Plasmid

   #58835

   Purpose

   Expresses GFP-Tagged Osh1 in yeast

   Depositor

   Tim Levine

   Article

   Loewen et al EMBO J. 2003 May 1;22(9):2025-35.

   Insert

   Osh1 (SWH1, OSH1 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   PHO5

   Available Since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pFA6a-mTID-HA3-HIS3MX6

   Plasmid

   #227983

   Purpose

   To insert biotin ligase, miniTurboID, at the C-terminus of genes; yeast BioID,HIS3MX6 marker

   Depositor

   Joseph Reese

   Article

   Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

   Insert

   miniTurboID
   

   Use

   Tags

   HA tag

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFA6a-HyTkAX

   Plasmid

   #73898

   Purpose

   fission yeast drug counter-selectable selection marker

   Depositor

   Kazunori Tomita

   Article

   Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

   Insert

   hyg-TK
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRMH122-LexA-CRY2PHR

   Plasmid

   #40613

   Purpose

   Expresses LexA-CRY2PHR for light-inducible protein expression when coupled with VP16-CIB

   Depositor

   Chandra Tucker

   Article

   Hughes et al Methods. 2012 Aug 15.

   Insert

   LexA-CRY2PHR
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available Since

   Oct. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pG/ER(G)

   Plasmid

   #108219

   Purpose

   Yeast expression vector for human estrogen receptor alpha

   Depositor

   Didier Picard

   Article

   Liu et al FEMS Microbiol Lett. 1998 Feb 15;159(2):

   Insert

   Human estrogen receptor a (ESR1 Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TDH3

   Available Since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFA6a- mYFP-kanMX6

   Plasmid

   #105147

   Purpose

   Yeast gene targeting

   Depositor

   Jian-Qiu Wu, Thomas Pollard

   Article

   Wu et al Dev Cell. 2003 Nov;5(5):723-34.

   Insert

   mYFP-kanMX6
   

   Use

   Tags

   mYFP

   Expression

   Bacterial and Yeast

   Mutation

   A206K

   Promoter

   Available Since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNXRVc-HACre

   Plasmid

   #78400

   Purpose

   constitutive Cre expression vector

   Depositor

   Kazunori Tomita

   Article

   Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

   Inserts

   3xHA-nls-Cre
   natMX6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p413TEF-PKM2

   Plasmid

   #34608

   Depositor

   Markus Ralser

   Article

   Bluemlein et al Oncotarget. 2011 May;2(5):393-400.

   Insert

   PKM2 (PKM Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF

   Available Since

   Jan. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. SJZ7-KAN

    Plasmid

    #228260

    Purpose

    Template plasmid for yeast gene knockout, confers antibiotic resistance to G418

    Depositor

    Zhiping Xie

    Article

    Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

    Insert

    kan
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPM1 S.c.

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. SJZ4-NAT

    Plasmid

    #228257

    Purpose

    Template plasmid for yeast gene knockout, confers antibiotic resistance to nourseothricin

    Depositor

    Zhiping Xie

    Article

    Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

    Insert

    nat1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    FBA1 S.c.

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pILGFP5EC4

    Plasmid

    #185880

    Purpose

    A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (9 copy)

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available Since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pILGFP3AG4ARSd

    Plasmid

    #185876

    Purpose

    A plasmid without an ARS sequence for in vivo gene amplification (HamAmp) at RPL25 locus (~12 copy)

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available Since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIM573

    Plasmid

    #74637

    Purpose

    alias pFA6a-ymTag-BFP-kanMX6 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and G418 selection marker

    Depositor

    Jiří Hašek

    Article

    Malcova et al FEMS Yeast Res. 2016 Mar 17. pii: fow027

    Insert

    ymTagBFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    none

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYSD300

    Plasmid

    #212930

    Purpose

    Encodes the S. cerevisiae Aga1 protein (Part3) with pGBK1 promoter, tENO2 terminator, HIS3 selectable marker and CEN/ARS yeast origin.

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    AGA1 (AGA1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. PHBA02

    Plasmid

    #83536

    Purpose

    Constitutive TEF1 promoter mediated expression of the UBiC and ARO4* genes

    Depositor

    Claudia Vickers

    Article

    Williams et al Metab Eng Commun. 2016 May 11;3:142-152.

    Insert

    pTEF1-ARO4*-CYC1t-pTEF1-UBiC-CYC1t
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pTEF1

    Available Since

    Dec. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJZC638

    Plasmid

    #62283

    Purpose

    Expresses MCP-VP64 and dCas9 in Yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    MCP-VP64
    dCas9
    

    Use

    Tags

    VP64

    Expression

    Yeast

    Mutation

    Nuclease activity has been deactivated by mutatio…

    Promoter

    pAdh and pGal10

    Available Since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. PhyB-mCherry-CAAX

    Plasmid

    #51567

    Purpose

    Constitutively express PM (Plasma membrane) localized PhyB in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PhyB (PHYB Mustard Weed)
    

    Use

    Tags

    CAAX and mCherry

    Expression

    Yeast

    Mutation

    1-908aa only

    Promoter

    ADH1pr

    Available Since

    Aug. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCTcon2-5INS_16

    Plasmid

    #102292

    Purpose

    Yeast Surface Display of 5Ins_16 scFV

    Depositor

    Sarel Fleishman

    Article

    Baran et al Proc Natl Acad Sci U S A. 2017 Oct 10;11

    Insert

    Insulin binding scFV
    

    Use

    Tags

    3x(G4S) Linker, G3S Linker, HA, and Myc

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFA6a-ymTurquoise2-CaURA3

    Plasmid

    #118458

    Purpose

    Yeast tagging vector to create a gene fusion with yeast optimized mTq2 with CaUra3 selection. *An updated version of this plasmid is available: #168057 pFA6a-link-ymTq2-URA3.

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge