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pACT-Lex

Plasmid

#183149

Purpose

Yeast-two-hybrid AD vector with 4XLexOp (activated by Lex-ER-B112)

Depositor

Assaf Mosquna

Article

Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

Insert

GAL4 AD

Use

Yeat two-hybrid

Tags

HA tag

Expression

Yeast

Mutation

Promoter

Available since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomKeima-Kan

Plasmid

#44902

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mKeima with Kan selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomKeima

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 17, 2013

Availability

Academic Institutions and Nonprofits only

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pGI3EM18

Plasmid

#135484

Purpose

Agrobacterium T-DNA with SpunH2Bpr driving hph-tdTomato

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

SpunH2Bpr hph-tdTomato ADH1t

Use

Tags

tdTomato

Expression

Bacterial and Yeast

Mutation

Promoter

SpunH2Bpr

Available since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pFTK020

Plasmid

#171292

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

PalcA syn_NoCrea (ANIA_08979) promoter

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pAG413 GAL ORF1 Shuffle1 NLS

Plasmid

#145794

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock

Article

Payero et al Journal of the South Carolina Academy of

Insert

ORF1 Shuffle1 NLS

Use

Tags

Expression

Yeast

Mutation

Enhanced Nucleur Localization Signal

Promoter

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAG425 GAL Pong TPase L418A, L420A

Plasmid

#145795

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock, Susan Wessler

Article

Hancock et al Mob DNA. 2010 Feb 1;1(1):5. doi: 10.1186

Insert

Pong TPase L418A, L420A

Use

Tags

Expression

Yeast

Mutation

L418A, L420A

Promoter

Available since

Jan. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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p415 Glo3

Plasmid

#112651

Purpose

Expression S.cerevisiae

Depositor

Blanche Schwappach

Article

Arakel et al J Cell Sci. 2019 Aug 29;132(16). pii: jc

Insert

Glo3

Use

Tags

Expression

Yeast

Mutation

Promoter

Met25

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1661

Plasmid

#112035

Purpose

OsTIR1 expression cassette marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

TIR1

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pBBK01 pCAS-Tyr-[gRNA: BsaI GFP dropout] (KanR, AmpR)

Plasmid

#178985

Purpose

Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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YIPlac204TKC-E2-Crimson-HDEL

Plasmid

#38771

Depositor

Benjamin Glick

Article

Strack et al Biochemistry. 2009 Sep 8;48(35):8279-81.

Insert

E2-Crimson

Use

Tags

Linker peptide and yeast ER retention signal sequ…

Expression

Yeast

Mutation

Promoter

TPI1

Available since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only

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p2B-57

Plasmid

#177927

Purpose

Expresses yeGFP under control of doxycycline-responsive TET3 promoter (contains three rtTA binding sites)

Depositor

Vadim Gladyshev

Article

Barre et al (unpublished)

Insert

EGFP

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

TET3 (contains three rtTA binding sites)

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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ZJOM156

Plasmid

#133636

Purpose

Integration of 2xGFP tag at SEC7 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast late golgi/early endosome.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

SEC7-2GFP (SEC7 Budding Yeast)

Use

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

Feb. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCEV-G2-Ph

Plasmid

#46816

Purpose

TEF1 and PGK1 promoter controlled expression cassettes with phleomycin resistance for yeast transformation

Depositor

Claudia Vickers, Lars Nielsen

Article

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Use

Tags

FLAG and c-myc

Expression

Yeast

Mutation

Promoter

TEF1 and PGK1

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pAG304GAL-ccdB

Plasmid

#14135

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 15, 2007

Availability

Academic Institutions and Nonprofits only

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p404-Grx5-GFP1-10

Plasmid

#91952

Purpose

Expresses GFP1-10 in the mitochondrial matrix

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Insert

Grx5-GFP1-10

Use

Tags

Expression

Yeast

Mutation

Promoter

GAP

Available since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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ZJOM25

Plasmid

#133672

Purpose

Integration of MSP1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on mitochondria and peroxisomes.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-MSP1-2GFP (MSP1 Budding Yeast)

Use

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fSNAP-URA

Plasmid

#188917

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fSNAP-URA

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pYC34

Plasmid

#63915

Purpose

Recipient overexpressing vector to construct mCherry tagged fusions

Depositor

Irene Castano Navarro

Article

Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

Type

Empty backbone

Use

Tags

mCherry

Expression

Yeast

Mutation

Promoter

TEF1 (Saccharomyces cerevisiae)

Available since

April 27, 2015

Availability

Academic Institutions and Nonprofits only

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dCas9v2

Plasmid

#103140

Purpose

TetR inducible dCas9-VPR plasmid with pRPR-NotI-tRPR1 Csy4-ready site

Depositor

Jens Nielsen

Article

Ferreira et al ACS Synth Biol. 2017 Nov 21. doi: 10.102

Insert

dCas9-VPR

Use

Tags

Expression

Yeast

Mutation

Removed scaffold gRNA from original plasmid

Promoter

Available since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pPEE47

Plasmid

#139426

Purpose

Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mNeonGreen ORF and a flexible linker at the 5' end.

Depositor

James Fraser

Article

Spencer et al Fungal Genet Biol. 2020 May;138:103365.

Insert

mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

May 18, 2020

Availability

Academic Institutions and Nonprofits only

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