7,998 results

1. pCfB2791

   Plasmid

   #63654

   Purpose

   pCfB2791 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.

   Depositor

   Jerome Maury, Irina Borodina

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Insert

   GFP
   

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1 promoter

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPICZ-pre-pro-alphaf(I)-E2-Crimson

   Plasmid

   #117660

   Purpose

   Test intracellular localisation of E2-Crimson and study the secretion efficiency

   Depositor

   Benjamin Glick, Pau Ferrer

   Article

   Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

   Insert

   pre-pro-alphaf(I)-E2-Crimson
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   This plasmid encodes the fluorescent protein E2-C…

   Promoter

   pAOX1

   Available since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDRF1 Cyterm-ytd8oxStayGold

   Plasmid

   #219853

   Purpose

   expression of Cyterm-ytd8oxStayGold in budding yeast

   Depositor

   Bas Teusink

   Insert

   Cyterm-ytd8oxStayGold
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. YIplac211-iGFP-TLG1

   Plasmid

   #105261

   Purpose

   Plasmid for pop-in/pop-out replacement of Tlg1 with iGFP-Tlg1.

   Depositor

   Benjamin Glick

   Article

   Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

   Insert

   Tlg1 (TLG1 Budding Yeast)
   

   Use

   Tags

   iGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDB4279

   Plasmid

   #98698

   Purpose

   A control plasmid containing the intact bsdMX marker

   Depositor

   Li-Lin Du

   Article

   Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAGE2.0-i

   Plasmid

   #194639

   Purpose

   Empty pAGE2.0 plasmid with pBAD inducible promoter

   Depositor

   Bogumil Karas

   Article

   Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

   Type

   Empty backbone

   Use

   Synthetic Biology; Diatom expression

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG426GAL-ccdB-DsRed

   Plasmid

   #14371

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   DsRed

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. LPH2781 - Ub-K63R

   Plasmid

   #32160

   Depositor

   Linda Hicke

   Insert

   synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   K63R

   Promoter

   CUP1

   Available since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pLexA::Ras

   Plasmid

   #11340

   Depositor

   Michael Wigler

   Article

   Chang et al Cell. 1994 Oct 7. 79(1):131-41.

   Insert

   Ras1 (ras1 Fission Yeast)
   

   Use

   Tags

   LexA DNA binding domain

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

10. pUG-amdSYM-mak10TR

    Plasmid

    #75433

    Purpose

    Generates truncated version of MAK10 gene, which is required for dsRNA L-A propagation in S.cerevisiae

    Depositor

    John McCusker

    Inserts

    MAK10TR
    ApR
    amdSYM
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    N-terminal 740 bp of MAK10 ORF

    Promoter

    Native promoter and PTEF

    Available since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKT0209

    Plasmid

    #8730

    Depositor

    Kurt Thorn

    Article

    Sheff et al Yeast 2004 Jun;21(8):661-70.

    Insert

    yEGFP
    

    Use

    Tags

    Codon optimized linker

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGBW-m4046708

    Plasmid

    #149173

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp4

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pBEVY-4420-mCherry

    Plasmid

    #218828

    Purpose

    Yeast surface display of an anti-fluorescene scFv and intracellular expression of mCherry

    Depositor

    Yongku Cho

    Article

    Arbaciauskaite et al Protein Eng Des Sel. 2023 Jan 21;36:gzad

    Insert

    4-4-20 scFv
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBEVY-pT231 scFv-GFP

    Plasmid

    #218827

    Purpose

    Yeast surface display of a phospho-tau pT231 scFv and intracellular expression of GFP

    Depositor

    Yongku Cho

    Article

    Arbaciauskaite et al Protein Eng Des Sel. 2023 Jan 21;36:gzad

    Insert

    pT231 tau scFv
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. BB3rN_14*

    Plasmid

    #98550

    Purpose

    empty BB3 for insertion and overexpression of 1 gene in P. pastoris

    Depositor

    Michael Sauer, Diethard Mattanovich, Brigitte Gasser

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pATP416-­ptetO7-­pphlO6-­crtYBI

    Plasmid

    #165976

    Purpose

    Expresses carotenoid biosynthesis gene in response to 2,4-diacetylphloroglucinol and doxycycline in yeast expressing rtTA and PhlTA

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Inserts

    Xanthophyllomyces dendrorhous phytoene-beta carotene synthase (crtYB) mRNA, complete cds
    Xanthophyllomyces dendrorhous phytoene desaturase mRNA, complete cds
    BTS1 (BTS1 Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    C1281A, G1698A and C66A, C1359T

    Promoter

    Synthetic promoter (pphlO6), Synthetic promoter (…

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. JME4390

    Plasmid

    #129656

    Purpose

    LEU2ex_CrisprCas9-yl_RFP: CAS9 vector with LEU2ex marker for gRNA cloning using GoldenGate

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Biotechnol Lett. 2020 Jan 23. pii: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pABY-c46

    Plasmid

    #201774

    Purpose

    Expresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeast

    Depositor

    Saravanan Palani

    Article

    Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

    Insert

    NbALFA
    

    Use

    Tags

    40aa Linker, mNeonGreen

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGFP-ATG13(315)

    Plasmid

    #122072

    Purpose

    Expressing GFP-Atg13

    Depositor

    Daniel Klionsky

    Article

    Chew et al Autophagy. 2015;11(6):891-905. doi: 10.1

    Insert

    ATG13
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRS402-nicastrin/APH-1a-HA

    Plasmid

    #207804

    Purpose

    Yeast integrative vector for expression of Nicastrin and APH-1a-HA

    Depositor

    Dieter Langosch

    Article

    Ortner et al J Mol Biol. 2023 Aug 1;435(18):168218. d

    Insert

    Gamma-secretase subunits nicastrin and Aph1a
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge