8,086 results

1. pCEV-G2-Km ymScarletI

   Plasmid

   #193960

   Purpose

   TEF1 promoter controlled expression plasmid with G418 resistance for expressing ymScarletI in budding yeast

   Depositor

   Bas Teusink

   Insert

   ymScarletI
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVG1

   Plasmid

   #111444

   Purpose

   Unified Solo vector pV1382 + sgScADE2 + ScADE2 stop codon repair tempate

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgScADE2/stop-codon repair
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. FRP935

   Plasmid

   #159302

   Purpose

   Yeast tagging vector to create gene fusion with yeast codon optimized mAmetrine under KanMX selection

   Depositor

   Joerg Stelling

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAG306GAL-ccdB

   Plasmid

   #14139

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. MAP72_MS2

   Plasmid

   #220623

   Purpose

   Cassette vector to enable overexpression of rNTases as MS2 coat protein fusions in S. cerevisiae.

   Depositor

   Marvin Wickens

   Article

   Preston et al Nat Methods. 2019 May;16(5):437-445. doi

   Type

   Empty backbone

   Use

   Shuttle vector

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pYC56

   Plasmid

   #63910

   Purpose

   Recipient integrative vector to construct mCherry tagged fusions

   Depositor

   Irene Castano Navarro

   Article

   Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

   Type

   Empty backbone

   Use

   Tags

   mCherry

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   April 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSE360-Prp22

   Plasmid

   #111284

   Purpose

   Prp22 WT gene in pSE360 backbone

   Depositor

   Christine Guthrie

   Article

   Noble et al Genetics. 1996 May;143(1):67-80.

   Insert

   PRP22
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. ZJOM157

   Plasmid

   #133640

   Purpose

   Integration of GFP-PEP12 as an additional copy in genome, use auxotrophic marker URA3(Kluyveromyces lactis).Marker for yeast late endosome.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-GFP-Pep12 (PEP12 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRG714_lexO-AscI_LexA-TF_URA3MX

   Plasmid

   #154822

   Purpose

   Inducible expression of AscI endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   AscI endonuclease
   LexA-ER-B112
   

   Use

   Tags

   FLAG and NLS

   Expression

   Yeast

   Mutation

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJAID5XY

    Plasmid

    #165051

    Purpose

    Integrative expression of mRFP-CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>mRFP-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    OsTIR1 is codon-optimised for expression in S. ce…

    Promoter

    Available Since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMAL728

    Plasmid

    #169152

    Purpose

    Encodes OptoQ-AMP6

    Depositor

    Jose Avalos

    Article

    Lalwani et al ACS Synth Biol. 2021 Aug 20;10(8):2060-2

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    A79Q

    Promoter

    Available Since

    Oct. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRS712

    Plasmid

    #213041

    Purpose

    CRISPR activation based overexpression in Nakaseomyces (Candida) glabrata

    Depositor

    Rebecca Shapiro

    Article

    Maroc et al mSphere. 2024 Feb 28;9(2):e0076123. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p413TEF1-ATR1(pYL573)

    Plasmid

    #178288

    Purpose

    Expresses NADPH-cytochrome P450 reductase 1 from A. thaliana (ATR1) in Saccharomyces cerevisiae. Centromeric HIS, PTEF1-ATR1-TCYC1

    Depositor

    Yanran Li

    Article

    Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

    Insert

    ATR1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFA6a-link-yoTagRFP657-SpHis5

    Plasmid

    #44846

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized TagRF657 with SpHis5 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoTagRFP657
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPT24

    Plasmid

    #184419

    Purpose

    Transformation of mitochondrial DNA in yeast

    Depositor

    Martin Ott

    Article

    Rzepka et al STAR Protoc. 2022 May 25;3(2):101359. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EZ-L545

    Plasmid

    #173024

    Purpose

    Encodes OptoEXP2 circuit

    Depositor

    Jose Avalos

    Article

    Zhao et al ACS Synth Biol. 2021 May 21;10(5):1143-1

    Insert

    VP16-EL222
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCfB6574

    Plasmid

    #106132

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pICXNHG3

    Plasmid

    #49021

    Purpose

    FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site

    Depositor

    Raimund Dutzler, Eric Geertsma

    Article

    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

    Type

    Empty backbone

    Use

    Tags

    3C protease site (PreScission site), GFP, and dec…

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available Since

    Jan. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. FRP1640_PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

    Plasmid

    #58440

    Purpose

    Cassette to replace yeast promoters with an inducible promoter containing 2 lexA boxes

    Depositor

    Joerg Stelling

    Article

    Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

    Insert

    PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PTEF-HygMX-TTEF-insul-(lexA-box)2-PminCYC1

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBS-SKII-3XHA-HALO-Hygromycin

    Plasmid

    #188929

    Purpose

    Integration cassette to create C-terminal fusions for fluorescent tagging

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    3XHA-HALO-Hygromycin
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge