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pPS3858

Plasmid

#113393

Purpose

galactose-inducible Ty3-his3AI; additional PPT

Depositor

Suzanne Sandmeyer

Insert

Ty3-his3AI

Tags

his3AI

Expression

Yeast

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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p112A1NE

Plasmid

#85983

Purpose

For construction of cDNA expression library.

Depositor

Wolf Frommer

Publication

Riesmeier et al EMBO J. 1992 Dec;11(13):4705-13.

Type

Empty backbone

Expression

Yeast

Promoter

Adh1

Available since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pAV105

Plasmid

#63195

Purpose

pRS405 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

Depositor

Jef Boeke

Publication

Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pROS15

Plasmid

#107929

Purpose

nat based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Publication

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y, gRNA-ADE2.Y

Use

CRISPR

Expression

Yeast

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pKP113 (pRS406-PRC1p-PRC1-GFP-ADH1t)

Plasmid

#106464

Purpose

Expresses Prc1 with a C-terminal GFP tag in yeast cells

Depositor

Daniel Klionsky

Publication

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

PRC1

Tags

GFP

Expression

Bacterial and Yeast

Promoter

PRC1

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB5791

Plasmid

#106126

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked Ura3 marker, integration into Yarrowia lipolytica chromosomal location IntF_2, USER site for cloning, amp resistance

Depositor

Irina Borodina

Publication

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCfB6633

Plasmid

#106162

Purpose

EasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6677 into Yarrowia lipolytica chromosomal location IntE_1, amp resistance

Depositor

Irina Borodina

Publication

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Insert

unknown

Expression

Yeast

Available since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMEL12

Plasmid

#107918

Purpose

hph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

Depositor

Jean-Marc Daran

Publication

Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

Insert

gRNA-CAN1.Y

Use

CRISPR

Expression

Yeast

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCfB4666

Plasmid

#106144

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistance

Depositor

Irina Borodina

Publication

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCfB6574

Plasmid

#106132

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistance

Depositor

Irina Borodina

Publication

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMVS142_pACT1_mCherry_Zif268_EBD_MCS_KAN

Plasmid

#99049

Purpose

Transcription Factor chassis for activation domains. mCherry, Zif268 DNA binding domain, estrogen response domain

Depositor

Barak Cohen

Publication

Staller et al Cell Syst. 2018 Mar 1. pii: S2405-4712(1

Inserts

ACT1 promoter (ACT1 Budding Yeast)
mCherry
Zif268 DNA binding Domain
Estrogen response domain (ESR1 Human)

Expression

Yeast

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pIMVAd40R

Plasmid

#98309

Purpose

Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of GAL promoters; integrated into ERG9 locus;

Depositor

Claudia Vickers

Publication

Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

Insert

ERG9C-terminal-TURA3- PGAL7>MVD1>TPRM9-PGAL2>-ERG12>TNATTIDP1

Expression

Yeast

Available since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pZMU-Dest

Plasmid

#105089

Purpose

Yeast expression vector for Gateway cloning and SUS bridge assays

Depositor

Christopher Grefen

Publication

Grefen et al Methods Mol Biol. 2014;1062:659-78. doi:

Type

Empty backbone

Tags

myc

Expression

Yeast

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-CHS3-msGFPx3

Plasmid

#105257

Purpose

Plasmid for pop-in/pop-out replacement of Chs3 with Chs3-msGFPx3.

Depositor

Benjamin Glick

Publication

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Chs3 portion (CHS3 Budding Yeast)

Tags

msGFPx3

Expression

Yeast

Available since

Feb. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pNXgate33-3HA

Plasmid

#105075

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

Depositor

Wolf Frommer

Publication

Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

Type

Empty backbone

Tags

3xHA and NubG

Expression

Yeast

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-GBP-hphMX6

Plasmid

#105159

Purpose

Yeast gene targeting

Depositor

Jian-Qiu Wu

Publication

Davidson et al Mol Biol Cell. 2015 Feb 1;26(3):453-66.

Insert

GBP-hphMX6

Tags

GBP

Expression

Bacterial and Yeast

Available since

Feb. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pYZ156

Plasmid

#98412

Purpose

Pombe Expression Vector - nmt41 promoter with Sp.ura4 marker

Depositor

Jef Boeke

Publication

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Expression

Yeast

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pYZ158

Plasmid

#98414

Purpose

Pombe Expression Vector - nmt1 promoter with Sp.leu1 marker

Depositor

Jef Boeke

Publication

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Expression

Yeast

Available since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-VPS10-msGFPx3

Plasmid

#105274

Purpose

Plasmid for pop-in/pop-out replacement of Vps10 with Vps10-msGFPx3.

Depositor

Benjamin Glick

Publication

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Vps10 portion (PEP1 Budding Yeast)

Tags

msGFPx3

Expression

Yeast

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pHES854

Plasmid

#89197

Purpose

Expresses mKate2 under control of TDH3 constitutive promoter.

Depositor

Hana El-Samad

Publication

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pTDH3-mKate2

Expression

Yeast

Promoter

TDH3

Available since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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