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pKP131 [pRS406-YBR139Wp-YBR139W(H474A)-GFP-ADH1t]

Plasmid

#106467

Purpose

Expresses Atg42/Ybr139w(H474A) with a C-terminal GFP tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W(H474A)

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Changed Histidine 474 to Alanine

Promoter

YBR139W

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKP133 [pRS406-YBR139Wp-YBR139W(D415A)-GFP-ADH1t]

Plasmid

#106468

Purpose

Expresses Atg42/Ybr139w(D415A) with a C-terminal GFP tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W(D415A)

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Changed Aspartate 415 to Alanine

Promoter

YBR139W

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKP134 [pRS406-YBR139Wp-YBR139W(S219,D415,H474A)-GFP-ADH1t]

Plasmid

#106469

Purpose

Expresses Atg42/Ybr139w(S219,D415,H474A) with a C-terminal GFP tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W(S219,D415,H474A)

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Changed Serine 219, Aspartate 415 and Histidine 4…

Promoter

YBR139W

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pKP135 (pRS406-YBR139Wp-YBR139W-PA-ADH1t)

Plasmid

#106470

Purpose

Expresses Atg42/Ybr139w with a C-terminal PA tag in yeast cells

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Insert

YBR139W

Tags

PA

Expression

Bacterial and Yeast

Promoter

YBR139W

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pUDP046

Plasmid

#107062

Purpose

Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting KU80 from Ogataea parapolymorpha

Depositor

Jean-Marc Daran

Article

Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

Insert

HH-gRNA-HDV targetting OpKU80 inO. parapolymorpha

Expression

Yeast

Promoter

ScTDH3

Available Since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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pBW106

Plasmid

#111138

Purpose

For the expression of rat AANAT with Met-Ser-Ile N-terminus in S. cerevisiae

Depositor

Alexander Varshavsky

Article

Wadas et al J Biol Chem. 2016 Aug 12;291(33):17178-9

Insert

MSI-ratAANAT-3FLAG (Leu2 deletion) (Aanat Rat)

Tags

3x Flag

Expression

Yeast

Mutation

Leu2 deletion

Promoter

Gal1

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pCHSU74.1-2.2

Plasmid

#110689

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

PKS

Tags

flag

Expression

Yeast

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pRS315Nab3-11

Plasmid

#110534

Purpose

Expresses yeast NAB3 using the endogenous promoter

Depositor

Daniel Reines

Article

Loya et al Nucleic Acids Res. 2012 Aug;40(15):7476-

Insert

NAB3 (NAB3 Budding Yeast)

Expression

Yeast

Mutation

two missense mutations creating Nab3-11

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCHSU70.1-2.2

Plasmid

#110682

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

PKS

Tags

flag

Expression

Yeast

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pCHSU70.2-2.2

Plasmid

#110683

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4

Expression

Yeast

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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