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Showing: 3121 - 3160 of 7821 results

  4. pGBW-m4046277

    Plasmid
    #145428
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145428-plasmid-map-sequence-id-298356

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  5. pJK371

    Plasmid
    #73596
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope and delta-12 fatty acid desaturase (FAD2-3) with C-terminal c-myc epitope
    Depositor
    T. J. Kappock
    Inserts
    delta-12 fatty acid acetylenase
    delta-12 fatty acid desaturase (form 3)
    Use
    Tags
    FLAG tag and c-myc tag
    Expression
    Yeast
    Mutation
    Point mutants in FAD2-3 ORF relative to deposited…
    Promoter
    GAL1 and GAL10
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73596-plasmid-map-sequence-id-139406

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  6. ymScarletI-ymNeongreen pDRF1-GW

    Plasmid
    #168067
    Purpose
    FRET fusion between ymScarletI and ymNeongreen for use in yeast
    Depositor
    Bas Teusink
    Insert
    ymScarletI-ymNeongreen
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    168067-plasmid-map-sequence-id-332110

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  8. pGBW-m4046319

    Plasmid
    #153866
    Purpose
    Yeast expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pGAL1
    Available since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153866-plasmid-map-sequence-id-300389

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  9. pGBW-m4046292

    Plasmid
    #149062
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;StrepII
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149062-plasmid-map-sequence-id-297979

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  12. pGBW-m4046249

    Plasmid
    #148991
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    148991-plasmid-map-sequence-id-297670

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  16. RPL25NLS-GFP-PRA (102)

    Plasmid
    #24041
    Depositor
    Michael Rout
    Insert
    RPL25 (RPL25 Budding Yeast)
    Use
    Tags
    EGFP and PRA
    Expression
    Yeast
    Mutation
    Only bp 1-135 (aa 1-45) of RPL25
    Promoter
    Available since
    May 3, 2010
    Availability
    Academic Institutions and Nonprofits only

  18. pTIN-ura4

    Plasmid
    #161808
    Purpose
    A ura-selectable lncRNA-regulated gene expression system with rapid induction kinetics in the fission yeast Schizosaccharomyces pombe
    Depositor
    Stewart Shuman
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Pho1
    Available since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    161808-plasmid-map-sequence-id-319459

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  19. pMM0647

    Plasmid
    #129008
    Purpose
    Integration vector for optogenetic machinery (pMEDIUM-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 marker
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    KanR-ColE1 URA 5'-pRPL18B-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1--pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Oct. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    129008-plasmid-map-sequence-id-259450

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  23. UASgal-A9-Pleum

    Plasmid
    #60343
    Purpose
    Contains a promoter of activating sequence derived from native gal promoter coupled with core promoter derived from native Leucine promoter. Spacer between core and activating sequence was used.
    Depositor
    Hal Alper
    Article
    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884
    Insert
    promoter
    Use
    Synthetic Biology; Yeast synthetic hybrid promote…
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 6, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    60343-plasmid-map-sequence-id-96621

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  24. pGBW-m4046230

    Plasmid
    #145454
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 ORF3a protein
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pTEF1
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145454-plasmid-map-sequence-id-300252

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  25. pCHKU38.2-2.2

    Plasmid
    #110670
    Purpose
    Expression of a fungal biosynthetic gene cluster
    Depositor
    Ronald Davis
    Article
    Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:
    Insert
    S_cerevisiae_ADH2-ORF1, S_bayanus_ADH2-ORF2, PCK1-ORF3, MLS1-ORF4, S_paradoxus_ADH2-ORF5, S_kudeiaveseii_ADH2-ORF6, ICL1-ORF7
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110670-plasmid-map-sequence-id-212351

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  29. pGBW-m4046658

    Plasmid
    #149226
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 nsp10
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 nsp10 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149226-plasmid-map-sequence-id-297800

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  30. pGBW-m4046682

    Plasmid
    #149197
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149197-plasmid-map-sequence-id-297817

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  31. pGBW-m4046642

    Plasmid
    #149164
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 nsp8
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 nsp8 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149164-plasmid-map-sequence-id-297810

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  32. pGBW-m4046687

    Plasmid
    #149148
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 nsp7
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 nsp7 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149148-plasmid-map-sequence-id-297804

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  37. pGBW-m4046268

    Plasmid
    #145423
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 M (membrane) (M Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pTEF1
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145423-plasmid-map-sequence-id-298350

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  38. pGBW-m4046216

    Plasmid
    #145490
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 M (membrane) (M Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145490-plasmid-map-sequence-id-298323

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  39. AausFP1-T2A-mCherry pdrf1-GW

    Plasmid
    #168071
    Purpose
    Produces equimolar expression of AausFP1 and mCherry for use in yeast
    Depositor
    Bas Teusink
    Insert
    AausFP1-T2A-mCherry
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    168071-plasmid-map-sequence-id-332147

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  40. pJT2

    Plasmid
    #98306
    Purpose
    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoter
    Depositor
    Claudia Vickers
    Article
    Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(
    Insert
    PTEF2>ScERG20N127W>TRPL3>PTEF1>AcNES1>TRPL41B
    Use
    Tags
    Expression
    Yeast
    Mutation
    ERG20 N127W
    Promoter
    Available since
    Feb. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    98306-plasmid-map-sequence-id-181809

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Showing: 3121 - 3160 of 7821 results