8,086 results

1. pTIN

   Plasmid

   #162885

   Purpose

   Overexpression vector for the fission yeast Schizosaccharomyces pombe

   Depositor

   Stewart Shuman

   Article

   Garg et al RNA. 2020 Nov;26(11):1743-1752. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFA6a-link-yEmCitrine-Hygro

   Plasmid

   #44645

   Depositor

   Kurt Thorn

   Insert

   yEmCitrine
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   A206R

   Promoter

   Available Since

   May 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYeDP60-hATP13A2-E343A

   Plasmid

   #171378

   Purpose

   A modified pYEDP60 plasmid containing a yeast codon-optimized version of human ATP13A2 variant 2 E343A mutant, followed by a thrombin cleavage site and a C-term BAD tag. DOI: 10.21769/BioProtoc.3888

   Depositor

   Peter Vangheluwe

   Article

   van Veen et al Nature. 2020 Feb;578(7795):419-424. doi:

   Insert

   human ATP13A2 variant 2 wild-type
   

   Use

   Tags

   BAD tag

   Expression

   Yeast

   Mutation

   E343A

   Promoter

   URA3

   Available Since

   Sept. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEasyG1_hph

   Plasmid

   #184912

   Purpose

   Template to generate via PCR a single gRNA for expression in S. cerevisiae

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAct::Raf

   Plasmid

   #11341

   Depositor

   Michael Wigler

   Article

   Van Aelst et al Proc Natl Acad Sci U S A. 1993 Jul 1. 90

   Insert

   byr-2 (byr2 Fission Yeast)
   

   Use

   Tags

   Gal4 activation domain

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pMJA089

   Plasmid

   #128518

   Purpose

   Expresses human Lysozyme in Pichia pastoris

   Depositor

   Marcos Alcocer

   Article

   Johnson et al J Mol Biol. 2005 Sep 30;352(4):823-36. d

   Insert

   Artificial gene for human lysozyme (LYZ Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   AOX1

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. RPL25-GFP (94)

   Plasmid

   #24037

   Depositor

   Michael Rout

   Insert

   RPL25 (RPL25 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   March 10, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pAG426GPD-EGFP-ccdB

   Plasmid

   #14324

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. p415GPD-3xHA-GW

   Plasmid

   #63781

   Purpose

   yeast expression vector p415GPD modified such that it is Gateway converted and introduces a 3xHA tag at the N-terminus of the insert

   Depositor

   Susan Baserga

   Article

   McCann et al Genes Dev. 2015 Apr 15;29(8):862-75. doi

   Type

   Empty backbone

   Use

   Tags

   3xHA

   Expression

   Yeast

   Mutation

   Promoter

   GPD

   Available Since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGBW-m4046203

    Plasmid

    #145488

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 3C-like proteinase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pE3c

    Plasmid

    #17463

    Purpose

    modified pENTR vector for C-terminal 6x MYC tag fusion with your gene of interest

    Depositor

    Giovanna Benvenuto

    Article

    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

    Type

    Empty backbone

    Use

    Modified gateway entry vector

    Tags

    6xMyc

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    Promoter

    Available Since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pXNubWtgate

    Plasmid

    #105080

    Purpose

    Yeast mating-based Split Ubiquitin Assay, positive control

    Depositor

    Wolf Frommer

    Article

    Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

    Type

    Empty backbone

    Use

    Tags

    NubI

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG306-GPD-AVT1 chr I

    Plasmid

    #41898

    Purpose

    Yeast expression of AVT1.

    Depositor

    Dan Gottschling

    Article

    Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

    Insert

    AVT1 (AVT1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available Since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPS2351

    Plasmid

    #1352

    Depositor

    Pamela Silver

    Article

    Hitchcock et al Mol Biol Cell 2001 Oct;12(10):3226-41.

    Insert

    MGA2 (MGA2 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

15. YEp112-CUP1-His-Smt3

    Plasmid

    #99537

    Purpose

    Yeast episomal vector for expression of His-tagged Smt3 (SUMO); TRP1 marker

    Depositor

    Helle Ulrich

    Article

    Ulrich et al Methods Mol Biol. 2009;497:81-103. doi:

    Insert

    SMT3 (SMT3 Budding Yeast)
    

    Use

    Tags

    7xHis

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRG211

    Plasmid

    #64519

    Purpose

    Centromeric E. coli/S. cerevisiae shuttle vector

    Depositor

    Joerg Stelling

    Article

    Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. YIplac211-iGFP-TLG1

    Plasmid

    #105261

    Purpose

    Plasmid for pop-in/pop-out replacement of Tlg1 with iGFP-Tlg1.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    Tlg1 (TLG1 Budding Yeast)
    

    Use

    Tags

    iGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. BB3rN_14*

    Plasmid

    #98550

    Purpose

    empty BB3 for insertion and overexpression of 1 gene in P. pastoris

    Depositor

    Diethard Mattanovich, Brigitte Gasser, Michael Sauer

    Article

    Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. M3927 ura3::KanMX3 Disruptor Converter

    Plasmid

    #51682

    Purpose

    Yeast marker swap plasmid for converting URA3 to KanMX3

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    KanMX3
    

    Use

    Yeast marker swap

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGAN201

    Plasmid

    #129204

    Purpose

    yTRAP sensor of [RNQ+], detects aggregation of Rnq1

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Insert

    RNQ1 (RNQ1 Budding Yeast, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SUP35

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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