8,086 results

1. pAV0751

   Plasmid

   #133485

   Purpose

   Expression in fission yeast from promoter pnmt1 , integrated at ura4 locus

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pUra4(AfeI)-p(nmt1)-sfGFP-terminator(ScCYC1)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAV0782

   Plasmid

   #133514

   Purpose

   Expression of sfGFP tagged LifeAct probe to monitor actin cytoskeleton

   Depositor

   Sophie Martin

   Article

   Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

   Insert

   pAde6(PmeI)-p(act1)-LifeAct-sfGFP-terminator(ScADH1)-bsdMX
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMJ235: pRS426-His-MBP-GtFz1_Native

   Plasmid

   #205254

   Purpose

   S. cerevisiae expression vector for GtFz1 native guide

   Depositor

   Feng Zhang

   Article

   Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

   Insert

   GtFz1
   

   Use

   Tags

   10xHis, MBP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGBK CUP1 mGrape3

   Plasmid

   #170265

   Purpose

   Yeast two-hybrid vector using a copper inducible CUP1 promoter, expressing mGrape3

   Depositor

   Bruce Hamilton

   Article

   Hernandez et al bioRxiv

   Insert

   momeric Grape 3
   

   Use

   Tags

   GAL4 DNA Binding Domain and Myc

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   CUP1

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-natMX6-P3nmt1-3FLAG

   Plasmid

   #19345

   Depositor

   Eishi Noguchi

   Article

   Noguchi et al Biotechnol J. 2008 Oct;3(9-10):1280-5. d

   Insert

   natMX6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Sept. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. ZJOM25

   Plasmid

   #133672

   Purpose

   Integration of MSP1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on mitochondria and peroxisomes.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-MSP1-2GFP (MSP1 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. M2660 ura3::LYS2 Disruptor Converter

   Plasmid

   #51678

   Purpose

   Yeast marker swap plasmid for converting URA3 to LYS2

   Depositor

   David Stillman

   Article

   Voth et al Yeast. 2003 Aug;20(11):985-93.

   Insert

   LYS2 (LYS2 Budding Yeast)
   

   Use

   Yeast marker swap

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pI2-MTU-DO-G418

   Plasmid

   #160018

   Purpose

   Integrative vector for K. marxianus, targeting integration site I2 described in (Rajkumar et al.,2019). G418 selection.

   Depositor

   John Morrissey

   Article

   Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

   Type

   Empty backbone

   Use

   Kluyveromyces marxianus

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   n/a

   Available Since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRS416 Gal M337V YFP

   Plasmid

   #27449

   Depositor

   Aaron Gitler

   Article

   Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

   Insert

   TDP-43 (TARDBP Human)
   

   Use

   Tags

   YFP

   Expression

   Yeast

   Mutation

   Methionine 337 mutated to Valine (M337V)

   Promoter

   Available Since

   Feb. 10, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pPICZ-pre-pro-alphaf-E2-Crimson

    Plasmid

    #117658

    Purpose

    Test intracellular localisation of E2-Crimson and study the secretion efficiency

    Depositor

    Pau Ferrer, Benjamin Glick

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pre-pro-alphaf-E2-Crimson
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    This plasmid encodes the fluorescent protein E2-C…

    Promoter

    pAOX1

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDRF1 mScarlet1.4-T2A-mTurquoise2

    Plasmid

    #219849

    Purpose

    equimolar expression of mScarlet1.4 and mTurquoise2 in budding yeast

    Depositor

    Bas Teusink

    Article

    Botman et al bioRxiv 2025.06.27.661975; doi: https://

    Insert

    mScarlet1.4-T2A-mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 27, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pML107-kanR468

    Plasmid

    #162041

    Purpose

    Expresses Cas9 and contains kanR468 guide which targets the kanR gene

    Depositor

    Jolanda Van Leeuwen

    Article

    Parts et al Mol Syst Biol. 2021 May;17(5):e10138. do

    Insert

    kanR468 guide
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG416GAL-ccdB-HA

    Plasmid

    #14243

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMEL10

    Plasmid

    #107916

    Purpose

    URA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pH3F-GAL1

    Plasmid

    #85775

    Purpose

    N-ICE plasmid pH3F-GAL1

    Depositor

    Scott Briggs

    Article

    Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

    Insert

    loxP-HphMX-loxP-GAL1p-3FLAG
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Jan. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAG416-GAL-FUS

    Plasmid

    #181711

    Purpose

    Galactose inducible expression of FUS

    Depositor

    Alejandro Chavez

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGBW-m4046708

    Plasmid

    #149173

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp4

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available Since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pGWKS10

    Plasmid

    #139417

    Purpose

    Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Clover marker.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    Clover
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available Since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFA6-mTFP1-KanMX6

    Plasmid

    #69851

    Purpose

    C-terminal tagging with mTFP1 using KanMX6 as a selection marker in S. cerevisiae.

    Depositor

    Liza Pon

    Article

    Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

    Insert

    mTFP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    April 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLexA::Ras

    Plasmid

    #11340

    Depositor

    Michael Wigler

    Article

    Chang et al Cell. 1994 Oct 7. 79(1):131-41.

    Insert

    Ras1 (ras1 Fission Yeast)
    

    Use

    Tags

    LexA DNA binding domain

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only