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8,350 results

Showing: 1621 - 1640 of 8350 results

  6. pScDD2_ERdd

    Plasmid
    #109047
    Purpose
    destabilizing domains for yeast (S. cerevisiae) derived from the human estrogen receptor ligand binding domain. This ERdd can be stabilized with 10 uM 4-hydroxytamoxifen (4-OHT, OHTAM).
    Depositor
    Thomas Wandless
    Insert
    ER50 (ESR1 H. sapiens gene with yeast enhanced codon)
    Tags
    yeast enhanced GFP with 8 amino acids TSGRGEGQ li…
    Expression
    Yeast
    Mutation
    3 mutations : E380G, R434G, N532S
    Promoter
    SOD1
    Available Since
    April 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    109047-plasmid-map-sequence-id-209173

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  9. PhyB-mCherry-Tom7

    Plasmid
    #66571
    Purpose
    Constitutively express mitochondrial localized PhyB in Saccharomyces cerevisiae
    Depositor
    Orion Weiner
    Article
    Jost et al ACS Synth Biol. 2015 Jun 2.
    Insert
    PhyB (PHYB Mustard Weed)
    Tags
    Tom7 and mCherry
    Expression
    Yeast
    Mutation
    PhyB portion contains aa's 1-908
    Promoter
    pADH1
    Available Since
    Oct. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    66571-plasmid-map-sequence-id-126395

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  14. ZJOM113

    Plasmid
    #133666
    Purpose
    Integration of VPS4-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-VPS4-mTagBFP2 (VPS4 Budding Yeast)
    Tags
    mTagBFP2
    Expression
    Yeast
    Available Since
    Jan. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    133666-plasmid-map-sequence-id-268963

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  16. pDB4281

    Plasmid
    #98700
    Purpose
    A Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX system
    Depositor
    Li-Lin Du
    Article
    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.
    Insert
    Cas9
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    98700-plasmid-map-sequence-id-182829

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  17. pDB4283

    Plasmid
    #98702
    Purpose
    A plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX system
    Depositor
    Li-Lin Du
    Article
    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.
    Insert
    gRNA PCR template
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Jan. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    98702-plasmid-map-sequence-id-182828

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  19. pCfB6576

    Plasmid
    #106134
    Purpose
    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_3, USER site for cloning, amp resistance
    Depositor
    Irina Borodina
    Article
    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    April 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    106134-plasmid-map-sequence-id-206167

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Showing: 1621 - 1640 of 8350 results