8,173 results
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Plasmid#234129PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
ExpressionYeastMutationIntron truncationAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 15bp dist
Plasmid#234128PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
ExpressionYeastMutationIntron truncationAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 42bp dist
Plasmid#234127PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
ExpressionYeastMutationIntron truncationAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 50bp dist
Plasmid#234112PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
ExpressionYeastMutationIntron truncationAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 46bp dist
Plasmid#234111PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
ExpressionYeastMutationIntron truncationAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6-CFAPoptim-NAT
Plasmid#221129PurposePlasmid for generating integration cassette to C-terminally tag gene of interest with FAPoptim::NATMXDepositorInsertFAPoptim
ExpressionYeastAvailable SinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFA6-CFAPoptim-KAN
Plasmid#221128PurposePlasmid for generating integration cassette to C-terminally tag gene of interest with FAPoptim::KANMXDepositorInsertFAPoptim
ExpressionYeastAvailable SinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
p413 TEF NAPstar2
Plasmid#232739PurposeExpresses NADPH/NADP+ biosensor NAPstar2 in S. cerevisiae.DepositorInsertNAPstar2
ExpressionYeastAvailable SinceApril 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
p413 TEF NAPstar4
Plasmid#232741PurposeExpresses NADPH/NADP+ biosensor NAPstar4 in S. cerevisiae.DepositorInsertNAPstar4
ExpressionYeastAvailable SinceApril 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
p413 TEF NAPstar6
Plasmid#232742PurposeExpresses NADPH/NADP+ biosensor NAPstar6 in S. cerevisiae.DepositorInsertNAPstar6
ExpressionYeastAvailable SinceApril 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
p413 TEF NAPstar7
Plasmid#232744PurposeExpresses NADPH/NADP+ biosensor NAPstar7 in S. cerevisiae.DepositorInsertNAPstar7
ExpressionYeastAvailable SinceApril 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-SEC61-FAPoptim
Plasmid#221126PurposeFAP-tagged marker for the ER (FAP optimized for yeast expression)DepositorInsertSEC61-FAPoptim
ExpressionYeastPromoterTEF1Available SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-SEC63-FAPoptim
Plasmid#221116PurposeFAP tagged SEC63 (C-terminally tagged, optimized) under the Tef1 promoter with 2xMYC tagDepositorInsertFAPoptim-SEC63
ExpressionYeastPromoterTEF1Available SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGBKT7_AvrSr62-5
Plasmid#233532PurposeExpresses AvrSr62-5 in yeastDepositorInsertAvrSr62-5
TagsGAL4 BD, Myc tagExpressionYeastPromoterADH1Available SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGBKT7_AvrSr62-1
Plasmid#233531PurposeExpresses AvrSr62-1 in yeastDepositorInsertAvrSr62-1
TagsGAL4 BD, Myc tagExpressionYeastPromoterADH1Available SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS415-TEF1pr-SEC61-FAPoptim
Plasmid#221115PurposeFAP tagged SEC61 (C-terminally tagged, optimized) under the Tef1 promoter with 2xMYC tagDepositorInsertSEC61-FAPoptim
ExpressionYeastPromoterTEF1Available SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCfb13198
Plasmid#219873PurposeThe base plasmid of TUNEYALI forTF08DepositorInsertContains gRNA targeting TF08 ( YALI1_D33640g) and homologous arm matching TF08
ExpressionYeastAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCTcon2-rHUH
Plasmid#235190PurposeTo display rHUH on yeast surfaceDepositorInsertrHUH
ExpressionYeastAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2643
Plasmid#229527PurposeCarries OsTIR1-t2a-mNG-mAID_opt, used to insert TIR1 expressing construct into the C. neoformans genome.DepositorInsertOsTIR1
Tagst2a-mNeonGreen-mAID_optExpressionBacterial and YeastMutationF74GAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCTcon2-rHUH_Y96F
Plasmid#235191PurposeTo display catalytic inactivated rHUH with Y96F mutation on yeast surfaceDepositorInsertrHUH_Y96F
ExpressionYeastAvailable SinceMarch 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCTcon2-rHUH_Y96F(G5)
Plasmid#235189PurposeTo display catalytic inactivated rHUH(G5) with Y96F mutation on yeast surfaceDepositorInsertrHUH_Y96F(G5)
ExpressionYeastAvailable SinceMarch 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCTcon2-rHUH(G5)
Plasmid#235188PurposeTo display rHUH(G5) on yeast surfaceDepositorInsertrHUH(G5)
ExpressionYeastAvailable SinceMarch 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCTcon2-dHUH
Plasmid#235187PurposeTo display dHUH on yeast surfaceDepositorInsertdHUH
TagsHA, aga2p, and mycExpressionYeastAvailable SinceMarch 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYI2294
Plasmid#228352PurposeMethanol-inducible ALX-0081 expressionDepositorInsertMFalpha(2xAdv)-LE-ALX-0081-3A-FLAG-His
UseAffinity Reagent/ AntibodyTagsAAA linker followed by FLAG-His6 and MF_ secretio…ExpressionYeastPromoterKpAOX1 promoterAvailable SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT2080
Plasmid#228339PurposeDoxycycline-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyExpressionYeastPromoterDoxycycline regulatable synthetic promoter (KpTc3…Available SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT2081
Plasmid#228340PurposeDoxycycline-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyExpressionYeastPromoterDoxycycline regulatable synthetic promoter (KpTc5…Available SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT2086
Plasmid#228341PurposeDoxycycline-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyExpressionYeastPromoterDoxycycline regulatable synthetic promoter (KpTc1…Available SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT2092
Plasmid#228342Purpose2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyExpressionYeastPromoter2,4-Diacetylphloroglucinol-regulatable synthetic …Available SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT2093
Plasmid#228343Purpose2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyExpressionYeastPromoter2,4-Diacetylphloroglucinol-regulatable synthetic …Available SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT2094
Plasmid#228344Purpose2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyExpressionYeastPromoter2,4-Diacetylphloroglucinol-regulatable synthetic …Available SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT2095
Plasmid#228345Purpose2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyExpressionYeastPromoter2,4-Diacetylphloroglucinol-regulatable synthetic …Available SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT2096
Plasmid#228346Purpose2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyExpressionYeastPromoter2,4-Diacetylphloroglucinol-regulatable synthetic …Available SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYI2206
Plasmid#228348PurposeConstitutive rPhlTA mutant expressionDepositorInsertphlTA mutant
UseSynthetic BiologyTagsThree copies of VP16 (VP48)-Nuclear localization …ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143KPromoterKpGAPDH promoterAvailable SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYI2216
Plasmid#228349Purpose2,4-Diacetylphloroglucinol (DAPG)-regulatable ALX-0081 expressionDepositorInsertMFalpha(2xAdv)-LE-ALX-0081-3A-FLAG-His
UseAffinity Reagent/ AntibodyTagsAAA linker followed by FLAG-His6 and MF_ secretio…ExpressionYeastPromoter2,4-Diacetylphloroglucinol-regulatable synthetic …Available SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYI2292
Plasmid#228350PurposeMethanol-inducible ALX-0171 expressionDepositorInsertMFalpha(2xAdv)-LE-ALX-0171-3A-FLAG-His
UseAffinity Reagent/ AntibodyTagsAAA linker followed by FLAG-His6 and MF_ secretio…ExpressionYeastPromoterKpAOX1 promoterAvailable SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYI2293
Plasmid#228351PurposeConstitutive ALX-0081 expressionDepositorInsertMFalpha(2xAdv)-LE-ALX-0081-3A-FLAG-His
UseAffinity Reagent/ AntibodyTagsAAA linker followed by FLAG-His6 and MF_ secretio…ExpressionYeastPromoterKpGAPDH promoterAvailable SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1487
Plasmid#228326Purpose2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyExpressionYeastPromoter2,4-Diacetylphloroglucinol-regulatable synthetic …Available SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1821
Plasmid#228327PurposeConstitutive mutant rTetTA expressionDepositorInsertrtetTA mutant
UseSynthetic BiologyExpressionYeastMutationK8NPromoterScPGK1 promoterAvailable SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1856
Plasmid#228328PurposeHomoserine lactone (HSL)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyExpressionYeastPromoterHomoserine lactone (HSL)-regulatable synthetic pr…Available SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1857
Plasmid#228329PurposeHomoserine lactone (HSL)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyExpressionYeastPromoterHomoserine lactone (HSL)-regulatable synthetic pr…Available SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1858
Plasmid#228330PurposeHomoserine lactone (HSL)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyExpressionYeastPromoterHomoserine lactone (HSL)-regulatable synthetic pr…Available SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1876
Plasmid#228331PurposeHomoserine lactone (HSL)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyExpressionYeastPromoterHomoserine lactone (HSL)-regulatable synthetic pr…Available SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1877
Plasmid#228332PurposeHomoserine lactone (HSL)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyExpressionYeastPromoterHomoserine lactone (HSL)-regulatable synthetic pr…Available SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1883
Plasmid#228333PurposeHomoserine lactone (HSL)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyExpressionYeastPromoterHomoserine lactone (HSL)-regulatable synthetic pr…Available SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1980
Plasmid#228334Purpose2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyExpressionYeastPromoter2,4-Diacetylphloroglucinol-regulatable synthetic …Available SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1981
Plasmid#228335Purpose2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyExpressionYeastPromoter2,4-Diacetylphloroglucinol-regulatable synthetic …Available SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT2013
Plasmid#228336PurposeDoxycycline-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyExpressionYeastPromoterDoxycycline regulatable synthetic promoter (KpPhl…Available SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT2076
Plasmid#228337Purpose2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyExpressionYeastPromoter2,4-Diacetylphloroglucinol-regulatable synthetic …Available SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT2077
Plasmid#228338Purpose2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyExpressionYeastPromoter2,4-Diacetylphloroglucinol-regulatable synthetic …Available SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1375
Plasmid#228314PurposeConstitutive expression of mutant rTetTADepositorInsertrtetTA mutant
UseSynthetic BiologyTagsThree copies of VP16 (VP48)ExpressionYeastMutationK8NPromoterKpGAPDH promoterAvailable SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only