8,058 results

1. p413TEF-SirT1

   Plasmid

   #51746

   Purpose

   overexpression of human SirT1 in S. cerevisiae, HIS3 marker

   Depositor

   Markus Ralser

   Article

   Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

   Insert

   SirT1 (SIRT1 Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJT11

   Plasmid

   #98308

   Purpose

   Overexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Citrus limon limonene synthase LIS1 under the control of GAL1/GAL2 promoter

   Depositor

   Claudia Vickers

   Article

   Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(

   Insert

   TRPL3ClLIS1>TRPL41B
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDS1209

   Plasmid

   #177340

   Purpose

   Expresses Aequorin in Candida albicans using the URA3 auxotrophic marker URA3

   Depositor

   Dominique Sanglard

   Article

   Sanglard et al J Fungi (Basel). 2021 Apr 20;7(4). pii:

   Insert

   Aequorin
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ACT1

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pXP622

   Plasmid

   #26849

   Depositor

   Nancy DaSilva, Suzanne Sandmeyer

   Article

   Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pESC-URA-hPOLISc

   Plasmid

   #60819

   Purpose

   to express human Pol iota recoded to yeast codons in yeast

   Depositor

   Youri Pavlov

   Article

   Makarova et al PLoS One. 2011 Jan 31;6(1):e16612. doi:

   Insert

   POLI (POLI Human)
   

   Use

   Tags

   c-myc

   Expression

   Yeast

   Mutation

   optimized to yeast codon usage

   Promoter

   GAL1

   Available since

   March 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGK401red-hENT1

   Plasmid

   #166028

   Purpose

   Expresses human equilibrative nucleoside transporter 1 (hENT1)

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

   Insert

   human equilibrative nucleoside transporter 1 (SLC29A1 Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pPGK1

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGALSc104(A503V)

   Plasmid

   #1152

   Depositor

   Susan Lindquist

   Article

   Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

   Insert

   HSP104 (HSP104 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   A503V

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAG416GPD-EGFP-ccdB

   Plasmid

   #14316

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRS416gT-GalL-Cas9

   Plasmid

   #79903

   Purpose

   pRS416 (URA) + RPR1(TetO) promoter with gRNA locus + Tetracycline Repressor + GalL promoter driven Cas9 for yeast expression

   Depositor

   Ronald Davis

   Article

   Fu et al Nucleic Acids Res. 2016 May 19. pii: gkw

   Inserts

   Cas9
   guide RNA (gRNA)
   Tetracycline Repressor
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GPM1 promoter, GalL, and RPR1 promoter with TetO …

   Available since

   July 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pV1081

    Plasmid

    #111425

    Purpose

    Solo vector for expression of CaCas9 and sgRNA for CaADE2 mutagenesis - integrates at ENO1

    Depositor

    Gerald Fink

    Article

    Vyas et al Sci Adv. 2015;1(3):e1500248.

    Insert

    CaCas9/sgADE-NT2
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDRFLIP36

    Plasmid

    #65514

    Purpose

    FRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.

    Depositor

    Wolf Frommer

    Article

    Jones et al eLife. 2014 Apr 15;3:e01741. doi: 10.755

    Type

    Empty backbone

    Use

    Tags

    6His-AFPt9-attR1 and attR2-Cer-cMyc

    Expression

    Yeast

    Mutation

    Promoter

    pPMA1

    Available since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDRf1-4CL5-DsRed (JBEI-15461)

    Plasmid

    #87935

    Purpose

    Cloning vector for co-expressing a gene with 4CL5 in S.cerevisiae

    Depositor

    Dominique Loque

    Article

    Eudes et al Microb Cell Fact. 2016 Nov 21;15(1):198.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pN3FS-CYC1

    Plasmid

    #85783

    Purpose

    N-ICE plasmid pH3FS-CYC1

    Depositor

    Scott Briggs

    Article

    Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

    Insert

    loxP-NatMX-loxP-CYC1p-3FLAG
    

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    *(see below)

    Promoter

    Available since

    Sept. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGAN200

    Plasmid

    #129203

    Purpose

    yTRAP sensor of [PSI+], detects aggregation of Sup35

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Insert

    Sup35NM (SUP35 Budding Yeast)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    N terminal and middle domains

    Promoter

    SUP35

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXNgate21-3HA

    Plasmid

    #105078

    Purpose

    Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

    Depositor

    Wolf Frommer

    Article

    Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

    Type

    Empty backbone

    Use

    Tags

    NubG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRS316CUP RNQ CFP

    Plasmid

    #1171

    Depositor

    Susan Lindquist

    Article

    Sondheimer et al Mol Cell 2000 Jan;5(1):163-72.

    Insert

    RNQ1 (RNQ1 Budding Yeast)
    

    Use

    Tags

    CFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 21, 2005

    Availability

    Academic Institutions and Nonprofits only

17. pHIS3p:yomRuby3-Tub1+3'UTR::LEU2

    Plasmid

    #204288

    Purpose

    yeast codon optimized mRuby3 alpha-tubulin plasmid for integration into the endogenous TUB1 ORF

    Depositor

    Linda Huang

    Article

    Seitz et al Mol Biol Cell. 2023 Sep 1;34(10):ar98. d

    Insert

    pHIS3p:yomRuby3-Tub1+3'UTR::LEU2
    

    Use

    Tags

    mRuby3

    Expression

    Yeast

    Mutation

    Promoter

    pHis3

    Available since

    July 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAV0471

    Plasmid

    #133490

    Purpose

    Expression of nucleo-cytoplasmic mTagBFP2

    Depositor

    Sophie Martin

    Article

    Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

    Insert

    pUra4(AfeI)-p(tdh1*)-mTagBFP2-terminator(tdh1)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p416GPD-PYK1

    Plasmid

    #34604

    Depositor

    Markus Ralser

    Article

    Gruning et al Cell Metab. 2011 Sep 7;14(3):415-27.

    Insert

    PYK1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available since

    Jan. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGBW-m4046409

    Plasmid

    #153939

    Purpose

    Yeast expression plasmid for SARS-CoV-2 S (surface glycoprotein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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