Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Yeast Expression Clear filter

Applied Filters

Yeast Expression Clear filter

8,350 results

Showing: 4901 - 4950 of 8350 results

  1. pMT1219

    Plasmid
    #228301
    Purpose
    2,4-Diacetylphloroglucinol (DAPG)-regulatable expression of SARS-CoV-2 RBD omicron variant
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    MFalpha(2xAdv)-SARS CoV-2 receptor binding domain (RBD)-His (S Synthetic, Severe acute respiratory syndrome-related coronavirus 2)
    Use
    Synthetic Biology
    Tags
    GGG linker followed by His6 and MF_ secretion sig…
    Expression
    Yeast
    Mutation
    G339D, S371L, S373P, S375F, K417N, N440K, G446S, …
    Promoter
    2,4-Diacetylphloroglucinol-regulatable synthetic …
    Available Since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228301-plasmid-map-sequence-id-457688

    Enlarge

  22. pMT1026

    Plasmid
    #228299
    Purpose
    Constitutive expression of PhlTA mutant with weak promoter and terminator
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    phlTA mutant
    Use
    Synthetic Biology
    Tags
    Three copies of VP16 (VP48)-Nuclear localization …
    Expression
    Yeast
    Mutation
    E41G
    Promoter
    KpYPT1 promoter
    Available Since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228299-plasmid-map-sequence-id-457081

    Enlarge

  25. pMT478

    Plasmid
    #228286
    Purpose
    Constitutive mutant rPhlTA expression with strong promoter
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    rphlTA mutant
    Use
    Synthetic Biology
    Tags
    Three copies of VP16 (VP48)-Nuclear localization …
    Expression
    Yeast
    Mutation
    P5S, S6P, K86T, F109L, Q117R, E143K
    Promoter
    KpGAPDH promoter
    Available Since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228286-plasmid-map-sequence-id-457102

    Enlarge

  27. pAG426-GAL-EWSR1

    Plasmid
    #181725
    Purpose
    Galactose inducible expression of EWSR1
    Depositor
    Alejandro Chavez
    Insert
    EWSR1 (EWSR1 Human)
    Expression
    Yeast
    Available Since
    March 27, 2025
    Availability
    Academic Institutions and Nonprofits only
    181725-plasmid-map-sequence-id-466838

    Enlarge

  31. pRS415-STE3pr-MFA-Igkappa-FAPoptim-STE3-TAILLESS

    Plasmid
    #221117
    Purpose
    FAP tagged STE3-TAILLESS mutant (delta288-470 - N-terminally tagged, optimized) under the Ste3 promoter with 2xMYC tag and MFA1 signal sequence to help target construct to the ER
    Depositor
    Allyson O’Donnell
    Article
    Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi
    Insert
    MFA-IgKappa-FAPoptim-STE3-Tailless
    Expression
    Yeast
    Mutation
    STE3 mutant that lacks the entire C-terminal tail…
    Promoter
    STE3
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    221117-plasmid-map-sequence-id-466649

    Enlarge

  33. Fyv6-NFLAG del1-51

    Plasmid
    #234136
    Purpose
    First 51 amino acids deleted of Fyv6 with FLAG epitope tag at N-terminus
    Depositor
    Aaron Hoskins
    Insert
    Fyv6
    Expression
    Yeast
    Mutation
    N-terminus truncation
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234136-plasmid-map-sequence-id-466538

    Enlarge

  34. pRS424 Prp8 S1584F

    Plasmid
    #234122
    Purpose
    Suppressor screen mutant in full-length Prp8 on a TRP plasmid
    Depositor
    Aaron Hoskins
    Insert
    Prp8
    Expression
    Yeast
    Mutation
    S1584F
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234122-plasmid-map-sequence-id-466537

    Enlarge

  35. pRS414-Syf1 817 Truncation

    Plasmid
    #234126
    Purpose
    Syf1 PCR truncation starting at 817 through the end of the gene
    Depositor
    Aaron Hoskins
    Insert
    Syf1
    Expression
    Yeast
    Mutation
    N-terminus truncation
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234126-plasmid-map-sequence-id-466534

    Enlarge

  36. pRS424 Prp8 S1584Y

    Plasmid
    #234121
    Purpose
    Suppressor screen mutant in full-length Prp8 on a TRP plasmid
    Depositor
    Aaron Hoskins
    Insert
    Prp8
    Expression
    Yeast
    Mutation
    S1584Y
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234121-plasmid-map-sequence-id-466533

    Enlarge

  37. pRS424 Prp8 V1862L

    Plasmid
    #234123
    Purpose
    Suppressor screen mutant in full-length Prp8 on a TRP plasmid
    Depositor
    Aaron Hoskins
    Insert
    Prp8
    Expression
    Yeast
    Mutation
    V1862L
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234123-plasmid-map-sequence-id-466526

    Enlarge

  40. Fyv6-NFLAG del1-16

    Plasmid
    #234138
    Purpose
    First 16 amino acids deleted of Fyv6 with FLAG epitope tag at N-terminus
    Depositor
    Aaron Hoskins
    Insert
    Fyv6
    Expression
    Yeast
    Mutation
    N-terminus truncation
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234138-plasmid-map-sequence-id-466525

    Enlarge

  41. Fyv6-NFLAG del103-173

    Plasmid
    #234134
    Purpose
    Amino acids 103-173 deleted in Fyv6 with FLAG epitope tag at N-terminus
    Depositor
    Aaron Hoskins
    Insert
    Fyv6
    Expression
    Yeast
    Mutation
    C-terminus truncation
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234134-plasmid-map-sequence-id-466524

    Enlarge

  42. pRS414-Fyv6-CFLAG

    Plasmid
    #234133
    Purpose
    Restriction digest from pUC18-CFLAG-Fyv6 and ligation into pRS414
    Depositor
    Aaron Hoskins
    Insert
    Fyv6
    Expression
    Yeast
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234133-plasmid-map-sequence-id-466523

    Enlarge

  43. Fyv6-NFLAG del134-173

    Plasmid
    #234135
    Purpose
    Amino acids 134-173 deleted in Fyv6 with FLAG epitope tag at N-terminus
    Depositor
    Aaron Hoskins
    Insert
    Fyv6
    Expression
    Yeast
    Mutation
    C-terminus truncation
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234135-plasmid-map-sequence-id-466521

    Enlarge

  44. pRS414-Fyv6

    Plasmid
    #234140
    Purpose
    Amplified Fyv6 from gDNA, cut with SalI and NotI, ligated into pRS414
    Depositor
    Aaron Hoskins
    Insert
    Fyv6
    Expression
    Yeast
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234140-plasmid-map-sequence-id-466520

    Enlarge

  45. pRS414-NFLAG-Fyv6

    Plasmid
    #234139
    Purpose
    Restriction digest from pUC18-NFLAG-Fyv6 and ligation into pRS414
    Depositor
    Aaron Hoskins
    Insert
    Fyv6
    Expression
    Yeast
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234139-plasmid-map-sequence-id-466518

    Enlarge

  46. pRS424 Prp8 G1868R

    Plasmid
    #234124
    Purpose
    Suppressor screen mutant in full-length Prp8 on a TRP plasmid
    Depositor
    Aaron Hoskins
    Insert
    Prp8
    Expression
    Yeast
    Mutation
    G1868R
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234124-plasmid-map-sequence-id-466515

    Enlarge

  47. pRS416-Fyv6

    Plasmid
    #234141
    Purpose
    Amplified Fyv6 from gDNA, cut with SalI and NotI, ligated into pRS416
    Depositor
    Aaron Hoskins
    Insert
    Fyv6
    Expression
    Yeast
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234141-plasmid-map-sequence-id-466513

    Enlarge

  48. Fyv6-NFLAG del1-23

    Plasmid
    #234137
    Purpose
    First 23 amino acids deleted of Fyv6 with FLAG epitope tag at N-terminus
    Depositor
    Aaron Hoskins
    Insert
    Fyv6
    Expression
    Yeast
    Mutation
    N-terminus truncation
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234137-plasmid-map-sequence-id-466511

    Enlarge

  49. pRS314-Cef1-M175I

    Plasmid
    #234118
    Purpose
    Suppressor screen mutant in Cef2
    Depositor
    Aaron Hoskins
    Insert
    Cef1
    Expression
    Yeast
    Mutation
    M175I
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234118-plasmid-map-sequence-id-466510

    Enlarge

  50. pRS314-Cef1-Q193P

    Plasmid
    #234117
    Purpose
    Suppressor screen mutant in Cef1
    Depositor
    Aaron Hoskins
    Insert
    Cef1
    Expression
    Yeast
    Mutation
    Q193P
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234117-plasmid-map-sequence-id-466508

    Enlarge

Showing: 4901 - 4950 of 8350 results