8,173 results
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Plasmid#231078PurposeMinimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker URA3DepositorTypeEmpty backboneUseIntegratingExpressionBacterial and YeastAvailable SinceFeb. 4, 2025AvailabilityAcademic Institutions and Nonprofits only
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pCas9-EcRT-Z3-Kan
Plasmid#232096PurposeYeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.DepositorInsertZ3 promoter and Z3EV transcription factor
UseCRISPRExpressionYeastPromoterZ3Available SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCfb13236
Plasmid#219909PurposeThe base plasmid of TUNEYALI for TF46DepositorInsertContains gRNA targeting TF46 ( YALI1_E20229g) and homologous arm matching TF46
ExpressionYeastAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLS405
Plasmid#231077PurposeMinimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker LEU2DepositorTypeEmpty backboneUseIntegratingExpressionBacterial and YeastAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLS404
Plasmid#231076PurposeMinimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker TRP1DepositorTypeEmpty backboneUseIntegratingExpressionBacterial and YeastAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLS408
Plasmid#231079PurposeMinimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker natMX6DepositorTypeEmpty backboneUseIntegratingExpressionBacterial and YeastAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLS403
Plasmid#231075PurposeMinimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker HIS3DepositorTypeEmpty backboneUseIntegratingExpressionBacterial and YeastAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLS400
Plasmid#231080PurposeMinimal integrating shuttle vector depleted of restriction sites outside the polylinker region, yeast selection marker KanMXDepositorTypeEmpty backboneUseIntegratingExpressionBacterial and YeastAvailable SinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pdnCas3-CDA1
Plasmid#229535PurposepMV_hyg encoding dnCas3(H74A+D75A)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloningDepositorInsertsdnCas3
Cytidine deaminase
Uracil glycosylase inhibitor
TagsXTENExpressionBacterial and YeastMutationcodon optimized for S.cerevisiae and codon optimi…Available SinceJan. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pdnCas3-APOBEC1
Plasmid#229537PurposepMV_hyg encoding dnCas3(H74A+D75A)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloningDepositorInsertsrAPOBEC1
dnCas3
Uracil glycosylase inhibitor
TagsXTENExpressionBacterial and YeastMutationcodon optimized for S.cerevisiae and codon optimi…Available SinceJan. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCas3-APOBEC1
Plasmid#229536PurposepMV_hyg encoding Cas3(wt)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloningDepositorInsertsrAPOBEC1
Cas3
Uracil glycosylase inhibitor
TagsXTENExpressionBacterial and YeastMutationcodon optimized for S.cerevisiae and codon optimi…Available SinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCas3-CDA1
Plasmid#229534PurposepMV_hyg encoding Cas3(wt)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloningDepositorInsertsCas3
Cytidine deaminase
Uracil glycosylase inhibitor
TagsXTENExpressionBacterial and YeastMutationcodon optimized for S.cerevisiae and codon optimi…Available SinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCfb13199
Plasmid#219874PurposeThe base plasmid of TUNEYALI forTF09DepositorInsertContains gRNA targeting TF09 (YALI1_F29652g) and homologous arm matching TF09
ExpressionYeastAvailable SinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCfb13218
Plasmid#219893PurposeThe base plasmid of TUNEYALI for TF28DepositorInsertContains gRNA targeting TF28 (YALI1_B28150g) and homologous arm matching TF28
ExpressionYeastAvailable SinceJan. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETcon-pGAL1-Aga2-α-Synuclein-c_myc
Plasmid#225222PurposeYeast optimized human alpha synuclein fused with aga2 protein gene under gal1 promoter for the expression of alpha synuclein in yeast surface display.DepositorInsertsα-Synuclein
Aga2
TagsGS linker, HA Tag, and c-myc TagExpressionYeastPromoterGAL1Available SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETcon-pGAL1-Aga2-Htt(25Q)-c_myc
Plasmid#225228PurposeYeast optimized human Htt (25Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (25Q) in yeast surface display.DepositorInsertsAga2
Htt (25Q)
TagsHA Tag and c-myc TagExpressionYeastAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETcon-pGAL1-Aga2-Htt(46Q)-c_myc
Plasmid#225229PurposeYeast optimized human Htt (46Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (46Q) in yeast surface display.DepositorInsertsAga2
Htt (46Q)
TagsHA Tag and c-myc TagExpressionYeastAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS316-SSD1-L1374
Plasmid#226278PurposePlasmid expressing the SSD1 allele from L-1374, under control of its native promoterDepositorInsertSSD1 (SSD1 Budding Yeast)
ExpressionYeastAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
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pVG178
Plasmid#227761PurposeExpresses a version of light-inducible transcription factor EL222 (Glu84Lys) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
ExpressionYeastMutationGlu84KLys mutantPromoterPGK1prAvailable SinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG144
Plasmid#227760PurposeExpresses a version of light-inducible transcription factor EL222 (Val121Met) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
ExpressionYeastMutationVal121Met mutantPromoterPGK1prAvailable SinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG150
Plasmid#227759PurposeExpresses a version of light-inducible transcription factor EL222 (Ser137Asn) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
ExpressionYeastMutationSer137Asn mutantPromoterPGK1prAvailable SinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG124
Plasmid#227758PurposeExpresses a version of light-inducible transcription factor EL222 (Thr83Ser) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
ExpressionYeastMutationThr83Ser mutantPromoterPGK1prAvailable SinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG121
Plasmid#227757PurposeExpresses a version of light-inducible transcription factor EL222 (Gly82Val) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
ExpressionYeastMutationGly80Glu mutantPromoterPGK1prAvailable SinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG123
Plasmid#227755PurposeExpresses a version of light-inducible transcription factor EL222 (Ala157Val) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
ExpressionYeastMutationAla157Val mutantPromoterPGK1prAvailable SinceJan. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG143
Plasmid#227754PurposeExpresses a version of light-inducible transcription factor EL222 (Leu87Ile) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
ExpressionYeastMutationLeu87IlePromoterPGK1prAvailable SinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG147
Plasmid#227753PurposeExpresses a version of light-inducible transcription factor EL222 (Gly94Cys) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
ExpressionYeastMutationGly94Cys mutantPromoterPGK1prAvailable SinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG120
Plasmid#227752PurposeExpresses a version of light-inducible transcription factor EL222 (Asp89Tyr) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
ExpressionYeastMutationAsp89Tyr mutantPromoterPGK1prAvailable SinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG137
Plasmid#227751PurposeExpresses a version of light-inducible transcription factor EL222 (Val201Leu) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
ExpressionYeastMutationVal201Leu mutantPromoterPGK1prAvailable SinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG136
Plasmid#227750PurposeExpresses a version of light-inducible transcription factor EL222 (Pro165Ser) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
ExpressionYeastMutationPro165Ser mutantPromoterPGK1prAvailable SinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG135
Plasmid#227749PurposeExpresses a version of light-inducible transcription factor EL222 (Lys99Glu) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
ExpressionYeastMutationLys99Glu mutantPromoterPGK1prAvailable SinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG119
Plasmid#227748PurposeExpresses a version of light-inducible transcription factor EL222 (Val139Leu) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
ExpressionYeastMutationVal139Leu mutantPromoterPGK1prAvailable SinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG118
Plasmid#227747PurposeExpresses a version of light-inducible transcription factor EL222 (Asp31Tyr) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
ExpressionYeastMutationAsp18Tyr mutantPromoterPGK1prAvailable SinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVG117
Plasmid#227746PurposeExpresses a version of light-inducible transcription factor EL222 (Asp31Tyr, Val139Leu) under a strong constitutive PGK1prDepositorInsertEL222 codon-optimized for budding yeast
ExpressionYeastMutationAsp18Tyr, Val139Leu double mutantPromoterPGK1prAvailable SinceJan. 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
YIplac128-TEF2p-LDH-CYC1t
Plasmid#216120PurposeYeast integrative vector for constitutive expression of LDH at the LEU2 locusDepositorAvailable SinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-TDH3p-LDH-CYC1t
Plasmid#216121PurposeYeast integrative vector for constitutive expression of LDH at the TRP1 locusDepositorAvailable SinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-TDH3p-CDC19-CYC1t
Plasmid#216122PurposeYeast integrative vector for constitutive expression of CDC19 at the TRP1 locusDepositorInsertPyruvate kinase 1
ExpressionYeastPromoterTDH3 promoterAvailable SinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMY.002
Plasmid#228681PurposepCCW12 Promoter for yeast expression with Nterminal tagDepositorInsertpCCW12
ExpressionYeastMutationWTAvailable SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOGS539_GLuc
Plasmid#226718PurposePlasmid enabling yeast-mediated expression and secretion of Gaussia luciferase (GLuc)DepositorInsertGaussia Luciferase
TagsHA tag and Mating factor alpha secretion signalExpressionYeastPromoterpTEF1Available SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMY.007
Plasmid#228686PurposepGAL1 Promoter for yeast expression with Nterminal tagDepositorInsertpGAL1
ExpressionYeastMutationWTAvailable SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMY.006
Plasmid#228685PurposepTEF2 Promoter for yeast expression with Nterminal tagDepositorInsertpTEF2
ExpressionYeastMutationWTAvailable SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMY.005
Plasmid#228684PurposepTEF1 Promoter for yeast expression with Nterminal tagDepositorInsertpTEF1
ExpressionYeastMutationWTAvailable SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMY.004
Plasmid#228683PurposepHHF2 Promoter for yeast expression with Nterminal tagDepositorInsertpHHF2
ExpressionYeastMutationWTAvailable SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMY.003
Plasmid#228682PurposepPGK1 Promoter for yeast expression with Nterminal tagDepositorInsertpPGK1
ExpressionYeastMutationWTAvailable SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMY.001
Plasmid#228680PurposepTDH3 Promoter for yeast expression with Nterminal tagDepositorInsertpTDH3
ExpressionYeastMutationWTAvailable SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfb13239
Plasmid#219912PurposeThe base plasmid of TUNEYALI for TF49DepositorInsertContains gRNA targeting TF49 (YALI1_C04143g) and homologous arm matching TF49
ExpressionYeastAvailable SinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tol2-TLCV2
Plasmid#196331Purposethe TLCV2 plasmid with Tol2 recombination sites for integrationDepositorInsertCas9-2A-eGFP
UseCRISPR; Fish expressionExpressionBacterial, Mammalian, and Y…PromoterTight TRE promoterAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC-His+Strep-Opa1 (SB254)
Plasmid#227603PurposeInducible coexpression of His-tagged SLC25A46 and Strep-tagged Opa1 in Pichia pastorisDepositorInsertsTags10xHis and Twin-StrepTagExpressionYeastMutationcontains aa 253-960 onlyPromoterAOX1Available SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC[∆2-83]-His+MFN2-Strep (SB259)
Plasmid#227608PurposeInducible coexpression of His-tagged SLC25A46 in its N-terminal region (∆2-83) and Strep-tagged MFN2 in Pichia pastorisDepositorTags10xHis and Twin-StrepTagExpressionYeastMutationaa 2-83 deletedPromoterAOX1Available SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC-His+MFN2[∆TM]-Strep (SB260)
Plasmid#227609PurposeInducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region in Pichia pastorisDepositorTags10xHis and Twin-StrepTagExpressionYeastMutationcontains aa 2-544, 651-757 only [TM deleted]PromoterAOX1Available SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only