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9,682 results

Showing: 2321 - 2340 of 9682 results

  3. pGTM_COV2_NSP5_C145A_001_SUMO

    Plasmid
    #192349
    Purpose
    Expresses SARS-CoV-2 3CLpro with an active site mutation (C154A) for the expression of the mature protease in an inactive form
    Depositor
    Gaetano Montelione
    Article
    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi
    Insert
    SARS-COV-2 Mpro-C145A (NEWENTRY SARS-CoV-2)
    Tags
    6X-His-Sumo
    Expression
    Bacterial
    Mutation
    Cysteine 145 of the active site is substituted wi…
    Promoter
    T7 lac
    Available Since
    June 3, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    192349-plasmid-map-sequence-id-381344

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  5. qTAG-N-Blast-3xFLAG-LMNB1

    Plasmid
    #207777
    Purpose
    Donor template for Blast-2A-3xFLAG insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LMNB1 Homology Arms flanking a Blast-3xFLAG Cassette (LMNB1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207777-plasmid-map-sequence-id-416194

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  7. pGBW-m4134905

    Plasmid
    #151966
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 N (Nucleocapsid)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 N (Nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available Since
    May 20, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    151966-plasmid-map-sequence-id-299794

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  10. mGeos2-VEL-PMP-N-10

    Plasmid
    #57557
    Purpose
    Localization: Peroxisomes, Excitation: 503 / 569, Emission: 514 / 581
    Depositor
    Michael Davidson
    Insert
    PMP (PXMP2 Human)
    Tags
    mGeos2-VEL
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 28, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. mEos3.2-Nup50-N-10

    Plasmid
    #57479
    Purpose
    Localization: Nuclear Pore, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    Nup50 (NUP50 Human)
    Tags
    mEos3.2
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  13. pGBW-m4046955

    Plasmid
    #145616
    Purpose
    Bacterial Expression plasmid for RNA-dependent RNA polymerase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    RNA-dependent RNA polymerase (Nsp12) (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145616-plasmid-map-sequence-id-307396

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  14. EPHA7 (2REI)

    Plasmid
    #25148
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    epha7.590.899 (EPHA7 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. pMCSG53_Nsp9

    Plasmid
    #167264
    Purpose
    Bacterial expression of Nsp9
    Depositor
    Alexei Savchenko
    Insert
    Nsp9 (residues 1-113) (ORF1ab SARS-CoV-2)
    Tags
    His6-TEV site
    Expression
    Bacterial
    Mutation
    none
    Available Since
    April 27, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    167264-plasmid-map-sequence-id-331338

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  16. AICSDP-078:G3BP1-mEGFP

    Plasmid
    #193920
    Purpose
    Homology arms and mEGFP-SGLRS for N-terminus tagging of human G3BP stress granule assembly factor 1
    Depositor
    Allen Institute for Cell Science
    Insert
    G3BP1 Homology Arms with mEGFP-SGLRS (G3BP1 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Dec. 22, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    193920-plasmid-map-sequence-id-380523

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  17. EHMT1 (2IGQ)

    Plasmid
    #25504
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    EHMT1 (EHMT1 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    C-terminal domain, residues 951-1235
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. mVenus-SiT-N-15

    Plasmid
    #56336
    Purpose
    Localization: Golgi TGN, Excitation: 515, Emission: 528
    Depositor
    Michael Davidson
    Insert
    SiT (ST6GAL1 Human)
    Tags
    mVenus
    Expression
    Mammalian
    Mutation
    aa 1-45 of SiT
    Promoter
    CMV
    Available Since
    Dec. 18, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. GABARAPL1

    Plasmid
    #25215
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ubh57.003.111 (GABARAPL1 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 12, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 2321 - 2340 of 9682 results