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9,723 results

Showing: 3281 - 3300 of 9723 results

  1. pCW-eGFP-SHLD2-m1

    Plasmid
    #114120
    Purpose
    Lentiviral vector for inducible expression of N-terminally tagged eGFP-SHLD2, m1 mutant
    Depositor
    Daniel Durocher
    Article
    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:
    Insert
    SHLD2 (m1 mutant) (SHLD2 Human)
    Use
    Lentiviral; Doxycycline inducible
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    W489A, F494A, W495A mutations introduced
    Promoter
    TRE promoter, Tet ON
    Available Since
    Aug. 28, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    114120-plasmid-map-sequence-id-220066

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  2. Spike Display_B.1.427/B.1.429

    Plasmid
    #172738
    Purpose
    Expression vector for SARS-CoV-2 (B.1.427/B.1.429) spike used in the Spike Display system
    Depositor
    IIya Finkelstein
    Article
    Javanmardi et al Mol Cell. 2021 Dec 16;81(24):5099-5111.e
    Insert
    SARS-CoV-2 S B.1.427/B.1.429 (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    3X-FLAG; Strep-Tag II; HRV 3C cut site; PDGFR-B T…
    Expression
    Mammalian
    Mutation
    Ectodomain only (AAs 1-1208); 682-685 (furin site…
    Promoter
    CMV
    Available Since
    Aug. 8, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    172738-plasmid-map-sequence-id-339247

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  3. Dronpa-H2B-6

    Plasmid
    #57277
    Purpose
    Localization: Nucleus/Histones, Excitation: 503, Emission: 518
    Depositor
    Michael Davidson
    Insert
    H2B (H2BC11 Human)
    Tags
    Dronpa
    Expression
    Mammalian
    Mutation
    D26G and V119I in H2B
    Promoter
    CMV
    Available Since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. mEmerald-ATG12-C-18

    Plasmid
    #54002
    Purpose
    Localization: Membrane Receptor, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    ATG12 (ATG12 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  5. TDRD3 (3PMT)

    Plasmid
    #28274
    Depositor
    Cheryl Arrowsmith
    Insert
    Tudor domain-containing protein 3 (TDRD3 Human)
    Tags
    His with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains Tudor domain, residues 553-611.
    Available Since
    April 14, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. pINER5

    Plasmid
    #185895
    Purpose
    In vivo gene amplification (HapAmp) of nerolidol synthetic module at RPL25 locus (15 copy).
    Depositor
    Claudia Vickers
    Article
    Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:
    Insert
    PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PSk.GAL2>YFAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2
    Expression
    Yeast
    Mutation
    None
    Available Since
    Aug. 25, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    185895-plasmid-map-sequence-id-370388

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  9. pGBW-m4046203

    Plasmid
    #145488
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 3C-like proteinase
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Tags
    Cleavable thrombin;3xFLAG
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pGAL1
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145488-plasmid-map-sequence-id-306276

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  10. pGBW-m4046758

    Plasmid
    #145670
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    wt
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145670-plasmid-map-sequence-id-297837

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  11. pOpen-EcoRIM

    Plasmid
    #165515
    Purpose
    Methylase recognizes double-stranded sequence GAATTC, methylates A-3 on both strands, and protects DNA from cleavage by EcoRI.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    M.EcoRI
    Use
    Synthetic Biology
    Available Since
    July 29, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165515-plasmid-map-sequence-id-338645

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  17. pDONR223 SARS-CoV-2 NSP14_nostop

    Plasmid
    #149316
    Purpose
    Gateway-compatible Entry vector, with insert of NSP14 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.
    Depositor
    Fritz Roth
    Article
    Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4
    Insert
    NSP14 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
    Use
    Gateway-compatible entry vector
    Mutation
    Many synonymous changes due to codon optimization
    Available Since
    May 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149316-plasmid-map-sequence-id-296465

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  18. pFHMSP-LIC-N

    Plasmid
    #26095
    Purpose
    Donor vector to generate recombinant baculovirus by site-specific transposition into a baculovirus shuttle vector For use with Bac-to-Bac Expression System insect cells for secreted protein expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Baculovirus expression
    Tags
    His and TEV cleavage site
    Expression
    Insect
    Promoter
    polyhedrin
    Available Since
    Sept. 15, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. pGBW-m4046409

    Plasmid
    #153939
    Purpose
    Yeast expression plasmid for SARS-CoV-2 S (surface glycoprotein)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable thrombin;StrepII
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pGAL1
    Available Since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153939-plasmid-map-sequence-id-300502

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  20. YPet-Actin-7

    Plasmid
    #56628
    Purpose
    Localization: Actin, Excitation: 513, Emission: 527
    Depositor
    Michael Davidson
    Insert
    Actin (ACTB Human)
    Tags
    YPet
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 18, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    56628-plasmid-map-sequence-id-175701

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Showing: 3281 - 3300 of 9723 results