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pcI-ts,ind+-(NSP8)

Plasmid

#160656

Purpose

Expresses codon optimized SARS-CoV-2 NSP8 in E. coli

Depositor

Kenneth Johnson

Article

Dangerfield et al iScience. 2020 Nov 28:101849. doi: 10.10

Insert

SARS-CoV-2 NSP8 (ORF1ab Synthetic)

Expression

Bacterial

Available Since

Dec. 10, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 MERS-CoV NSP6_stop

Plasmid

#168633

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

NSP6 (orf1ab )

Use

Other

Available Since

July 5, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 MERS-CoV NSP3_nostop

Plasmid

#168608

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

NSP3 (orf1ab )

Use

Other

Available Since

Jan. 20, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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BASIC_SEVA_5a7_pKD46.10

Plasmid

#178937

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Article

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Available Since

Feb. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC25A26

Plasmid

#132259

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A26 (SLC25A26 Human)

Expression

Mammalian

Available Since

Nov. 19, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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mCardinal-H1-10

Plasmid

#56161

Purpose

Localization: Nucleus/Histones, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

H1 (H1f0 Mouse)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
mTagRFP-T-Gamma-Tubulin-17

Plasmid

#58017

Purpose

Localization: Centrosomes, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Gamma-Tubulin (TUBG1 Human)

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mVenus-UbiquitinC-N-18

Plasmid

#56339

Purpose

Localization: Membrane , Excitation: 515, Emission: 528

Depositor

Michael Davidson

Insert

UbiquitinC (UBA52 Human)

Tags

mVenus

Expression

Mammalian

Mutation

aa 2-76 of NP_003324.1

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221-SLC25A29_STOP

Plasmid

#161194

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A29 (SLC25A29 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pMD19_STARR-seq_plants

Plasmid

#117379

Purpose

STARR-seq vector with 35s core promoter for transient expression in plant protoplasts

Depositor

Xiaoyu Zhang

Article

Ricci et al Nat Plants. 2019 Nov 18. pii: 10.1038/s4

Type

Empty backbone

Tags

GFP derived from pMDC107

Expression

Plant

Promoter

35s core promoter

Available Since

Nov. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX4T1-Smurf1 wt

Plasmid

#13502

Depositor

Jeff Wrana

Article

Zhu et al Nature. 1999 Aug 12. 400(6745):687-93.

Insert

SMURF1 (SMURF1 Human)

Tags

GST

Expression

Bacterial

Available Since

Dec. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_MFSD2A

Plasmid

#131893

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

MFSD2A (MFSD2A Human)

Expression

Mammalian

Available Since

Oct. 9, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pOpen-BsupolLF

Plasmid

#165563

Purpose

Bsu DNA Polymerase I, Large Fragment retains the 5'-3' polymerase activity of the Bacillus subtilis DNA polymerase I, but lacks the 5'-3' exonuclease domain. This large fragment naturally lacks 3'-5' exonuclease activity. Applications include random primer labeling, second strand cDNA synthesis, single dA tailing, and strand displacement DNA synthesis.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

Bsu DNA Polymerase I, Large Fragment

Use

Synthetic Biology

Available Since

Aug. 17, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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CCT8_pCSdest

Plasmid

#53795

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

CCT8 (CCT8 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

Sept. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mEmerald-Desmin-N-18

Plasmid

#54060

Purpose

Localization: Intermediate Filaments, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

Desmin (DES Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pENTR221 LRRK2 wt

Plasmid

#39529

Depositor

Michael J Fox Foundation MJFF

Insert

LRRK2 (LRRK2 Human)

Use

Gateway compatible cloning vector

Available Since

Oct. 1, 2012

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-CMV-Galpha-s-IRESWT-Gbetagamma ONE-GO

Plasmid

#204340

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

Galpha-s/Gbetagamma ONE-GO

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pOpen-PstIM

Plasmid

#165536

Purpose

Methylase that recognizes the dsDNA sequence CTGCAG, methylates on A-5 on both strands, and protects DNA from cleavage by PstI.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

M.PstI

Use

Synthetic Biology

Available Since

Aug. 5, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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Sirius-N1

Plasmid

#54506

Purpose

Localization: N1 Cloning Vector, Excitation: 355, Emission: 424

Depositor

Michael Davidson

Article

Tomosugi et al Nat Methods. 2009 May;6(5):351-3. doi: 1

Type

Empty backbone

Tags

Sirius

Expression

Mammalian

Available Since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pTipi2.1_Anti-ProC [IPI-HPC-4] Mouse/Rabbit HC

Plasmid

#221361

Purpose

Mammalian expression of Anti-ProC [IPI-HPC-4] Mouse/Rabbit HC; to be used with Anti-ProC [IPI-HPC-4] light chain (Plasmid 221362) to make the antibody.

Depositor

Institute for Protein Innovation (IPI)

Insert

Anti-ProC [IPI-HPC-4] Mouse/Rabbit HC

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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