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Plasmid#39173PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertGHRHR (GHRHR Human)
UseBaculovirus expressionTagsSignal-His6-TEVExpressionMutationN20->Q mutation of putative glycosylsation sit…PromoterAvailable sinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
GHRHR-Tango
Plasmid#66292PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertGHRHR (GHRHR Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
GHR1 X017_pECIA2
Plasmid#115047PurposeBait vector GHR1 X017_pECIA2 should be used with prey vector GHR1 X017_pECIA14.DepositorInsertAT4G20940
UseTagsFc, V5 and hexahistidine tagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
GHR1 X017_pECIA14
Plasmid#114847PurposePrey vector GHR1 X017_pECIA14 should be used with bait vector GHR1 X017_pECIA2.DepositorInsertAT4G20940 (GHR1 Mustard Weed)
UseTagsExpressionInsectMutationPromoterMetallothionein (Copper-Inducible)Available sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXPR_016-sgHRI
Plasmid#202443PurposeExpression of a guide RNA targeting HRIDepositorInsertHRI gRNA (EIF2AK1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHD-dsRed-attP_dCrk_flankingHRs
Plasmid#131141PurposeContains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).DepositorUseCRISPR and Cre/Lox; Homology directed repair temp…TagsExpressionMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSGDlucV3.0
Plasmid#119760Purposedual luciferase with FMDV StopGo sequences flanking MCSDepositorTypeEmpty backboneUseLuciferaseTagsExpressionMammalianMutationPromoterSV40 and T7Available sinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
U6-sgRNA-pcr4
Plasmid#193662PurposeExpression of tandem pre-sgRNA array pcr4 for LbCas12aDepositorInsertU6-PUM1-sgRNA-GHR-sgRNA-HMGA2-sgRNA-PUM2-sgRNA-tRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVLAD6
Plasmid#41850DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pVLAD8L
Plasmid#41853DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2013AvailabilityAcademic Institutions and Nonprofits only